Fataty, Rihma Fathin (2018) Pengaruh Kasein Yogurt Susu Kambing Terhadap Kadar Malondialdehida (MDA) dan Aktivitas Protease Jejunum Tikus (Rattus norvegicus) Hasil Induksi 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Sarjana thesis, Universitas Brawijaya.
Abstract
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) merupakan golongan senyawa hidrokarbon aromatik terhalogenasi yang berbahaya dan mampu terakumulasi dalam jangka panjang di dalam tubuh. Akumulasi TCDD di dalam tubuh dapat meningkatkan stres oksidatif akibat peroksidasi lipid. Kasein yogurt susu kambing peranakan etawa diketahui memiliki kandungan biopeptida aktif yang berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh kasein yogurt susu kambing dalam menangani keracunan TCDD yang dapat diamati melalui biomarker kadar Malondialdehida (MDA) dan aktivitas protease. Hewan coba yang digunakan yaitu tikus Rattus norvegicus jantan usia 8-12 minggu dengan berat 150-250 gram sebanyak 20 ekor yang dibagi menjadi lima kelompok perlakuan yaitu kelompok negatif, kelompok positif, kelompok terapi dengan dosis 300 mg/kg BB, 600 mg/kg BB, dan 900 mg/kg BB. Induksi TCDD diberikan sebanyak 100 ng/kg BB/hari melalui sonde lambung selama 21 hari. Terapi yogurt susu kambing dalam bentuk freeze dry melalui sonde lambung selama 21 hari. Pengukuran kadar Malondialdehida (MDA) dilakukan menggunakan metode TBA (Thiobarbituric Acid) sedangkan pengukuran aktivitas protease menggunakan metode Walter. Hasil penelitian menunjukkan bahwa terapi yogurt susu kambing secara signifikan (p<0.01) dapat menurunkan kadar Malondialdehida (MDA) dan aktivitas protease. Ketiga dosis memiliki potensi yang sama dalam menurunkan kadar Malondialdehida (MDA) dan aktivitas protease. Kesimpulan dari penelitian ini adalah kasein yogurt susu kambing dapat digunakan sebagai nutrasetika alami untuk penanganan keracunan TCDD.
English Abstract
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a class of harmful, halogenated, aromatic hydrocarbon compound which can accumulate inside the body from time to time. The accumulation of TCDD inside the body can increase oxidative stress due to lipid peroxidation. Goat milk yogurt casein contains active biopeptides which can be considered as potential antioxidant. This research aimed to know the influence of goat milk yogurt casein in reducing the effect of TCDD, i.e. poisoning that can be observed through biomarker level of Malondialdehyde (MDA) and protease activity. This research involved 20 males of Rattus norvegicus as a guinea pig, ranged in age from 8 to 12 weeks and weights from 150 to 250 grams. They were divided into five groups of treatment: negative group, positive group, therapy group with doses of 300 mg/kg BW, 600 mg/kg BW, and 900 mg/kg BW. TCDD induction was administered as much as 100 ng/kg BW/day through gastrostomy feeding tube for 21 days. Goat milk yogurt therapy was introduced using freeze dry yogurt through gastrostomy feeding tube for 21 days. The measurement of Malondialdehyde level (MDA) was carried out using TBA (Thiobarbituric Acid) method, while the protease activity was measured using Walter method. The result showed that goat milk yogurt therapy significantly decreased the level of Malondialdehyde (MDA) (p <0.01) and the protease activity. All the three doses had the same potential in decreasing the level of Malondialdehyde (MDA) and the protease activity. It can be concluded that goat milk yogurt casein can be used as natural nutraceutic in reducing the effect of TCDD, i.e. poisoning.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/281/051807041 |
| Uncontrolled Keywords: | TCDD, kasein, yogurt susu kambing, malondialdehida, aktivitas protease, TCDD, casein, goat milk yogurt, malondialdehyde, protease activity |
| Subjects: | 600 Technology (Applied sciences) > 641 Food and drink > 641.3 Food > 641.37 Dairy and related products > 641.371 476 Yogurt |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 27 May 2020 16:26 |
| Last Modified: | 22 Oct 2021 08:29 |
| URI: | http://repository.ub.ac.id/id/eprint/168392 |
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