Pratama, Farhan Adi (2019) Pengaruh Pemberian Amlodipin Terhadap Ekspresi MAP2 pada Kultur Neuron SH-SY5Y yang Diinduksi Hiperglikemia Kronis. Sarjana thesis, Universitas Brawijaya.
Abstract
Hiperglikemi adalah kadar glukosa darah lebih dari 240 mg/dl disertai dengan penurunan sekresi insulin dan kerja insulin atau keduanya. Pada sel kultur dikatakan hiperglikemi apabila pemberian glukosa dalam dosis antara 25 mM – 100 mM. Akibat dari kondisi hiperglikemi terjadi peningkatan ion Ca2+ sitosol yang menyebabkan disfungsi pada mitokondria. Akumulasi dari ion Ca2+ menyebabkan meningkatnya stress oksidatif. Stres oksidatif akan meningkatkan aktivitas calpain (Calcium Activated Neural Protease) menyebabkan MAP2 (Microtubule Associated Protein 2) menurun sehingga akan meningkatkan kematian sel. MAP2 merupakan protein sitoskeletal yang berikatan dengan aktin dan filamen di mikrotubulus yang berfungsi dalam diffensiasi dan plastisitas neuron. Pemberian amlodipin yang berikatan dengan reseptor L-type Calcium Channel (LTCC) dapat mengurangi influks ion Ca2+ intraseluler. Berkurangnya influks ion Ca2+ intraseluler akan menurunkan stres oksidatif dan aktivitas calpain sehingga dapat meningkatkan MAP2. Penelitian ini bertujuan untuk melihat efek pemberian amlodipin terhadap ekspresi MAP2 pada sel kultur neuron SH-SY5Y. Penelitian ini menggunakan pendekatan uji normalitas, uji anova dan uji korelasi spearman. Bedasarkan hasil uji anova pada pemberian amlodipin 10 nM, 100 nM dan tanpa amlodipin terhadap ekspresi MAP2 pada kultur neuron SH-SY5 yang diinduksi glukosa 50 mM selama 6 hari terjadi penurunan MAP2 (p= 0,082) artinya, pemberian amlodipin 10 nM, 100 nM dan tanpa pemberian amlodipin tidak memiliki pengaruh terhadap MAP2. Berdasarkan hasil uji korelasi spearman terdapat hubungan yang kuat (p=0,011) (r=-0,791) artinya semakin tinggi pemberian dosis amlodipin akan menurunkan ekspresi MAP2.
English Abstract
Hyperglycemia is a blood glucose level of more than 240 mg / dl ,accompanied by a decrease in insulin secretion and insulin action or both. In culture cells it is said to be hyperglycemic when administering glucose in doses between 25 mM - 100 mM. As a result of hyperglycemic conditions there is an increase in cytosolic Ca2 + ions which cause mitochondrial dysfunction. The accumulation of Ca2+ ions causes increased oxidative stress. Oxidative stress will increase the activity of Calcium Activated Neural Protease causing MAP2 (Microtubule Associated Protein 2) to decrease which will increase the cell death. MAP2 is a cytoskeletal protein that binds to actin and filaments in microtubules that function in diffrentiation and plasticity of neurons. The administration of amlodipine which binds to the L-type Calcium Channel receptor (LTCC) can reduce the intracellular Ca2+ ion influx. The reduced intracellular Ca2+ ion influx will reduce oxidative stress and Calcium Activated Neural Protease activity, and caused the MAP2 increases. The aim of research is to determine on the effects of amlodipine on the expression of MAP2 in SH-SY5Y neuron culture cells. This study uses the normality test, ANOVA test and spearman correlation test. Based on the results of the Anova test on the administration of 10 nM, 100 nM and without amlodipine on the expression of MAP2 in culture of SH-SY5 neurons, which were 50 mM glucose for 6 days there was decrease in MAP2 (p = 0.082) means that administration of amlodipine 10 nM, 100 nM and without amlodipine did not have effect in MAP2. Based on Spearman correlation test results, there was a strong relationship (p = 0.011) (r = -0.7791) means that the higher the dose of amlodipine, the lower the MAP2 expression.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/254/051909453 |
| Uncontrolled Keywords: | MAP2, amlodipin, hiperglikemi, Kultur neuron SH-SY5Y, MAP2, amlodipine, hyperglycemia, SH-SY5Y neuron culture |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics > 613.28 Specific nutritive elements > 613.282 Proteins |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 26 Aug 2020 04:42 |
| Last Modified: | 25 Oct 2021 03:12 |
| URI: | http://repository.ub.ac.id/id/eprint/176240 |
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