Permata, Novilla Anindya (2018) Pengaruh Pemberian Tepung Kulit Mangga Manalagi (Mangifera Indica L.) Terhadap Aktivitas Enzim SOD Serum Tikus Putih (Norvegicus Strain Wistar) Jantan Yang Diberi Diet Tinggi Lemak. Sarjana thesis, Universitas Brawijaya.
Abstract
Diet tinggi lemak dapat menurunkan aktivitas enzim SOD serum. Maka, diperlukan antioksidan eksogen yang dapat didapatkan dari tepung kulit mangga manalagi yang mengandung 181,4110 ± 3,818 mg/L total fenol. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tepung kulit mangga manalagi terhadap aktivitas enzim SOD serum. Studi eksperimental menggunakan post test control group design yang dilakukan terhadap tikus Wistar jantan. Sampel dibagi dalam lima kelompok, dengan masing-masing kelompok 5 tikus namun, berjalannya waktu penelitian selama 42 hari terdapat tikus yang mati terutama P3 yang mendapatkan dosis 1,6 g tepung mati semua sehingga tersisa K (-) 5 ekor, K (+) 5 ekor, P1 dosis 0,4 g tepung 4 ekor, dan P2 dosis 0,8 g tepung 3 ekor. Intervensi tepung kulit mangga manalagi dibuat dengan cara pengeringan pada oven suhu 100ºC selama 3 jam. Variabel yang diukur adalah aktivitas enzim SOD serum dengan metode spektofotometri. Didapatkan hasil lab ratarata aktivitas enzim SOD serum yaitu K (-) 27,378 U/mL, K (+) 25,778 U/mL, P1 26,278 U/mL, dan P2 25,630 U/mL. Berdasarkan hasil uji one way ANOVA, didapatkan hasil p=0,623 (p>0,05) yang menunjukkan bahwa tidak adanya perbedaan data aktivitas enzim SOD serum pada keempat kelompok perlakuan atau hasil tidak signifikan. Dimana rata-rata aktivitas enzim SOD serum pada P2 mendekati K (-) yang dapat dikarenakan adanya prooksidasi polifenol.
English Abstract
diet high in fat can lower serum SOD enzyme activity. Then, the exogenous antioxidants needed can be obtained from flour containing manalagi mango skin 181.4110 ± 3.818 mg/L total phenol. This research aims to know the influence of the giving of the flour skin against manalagi mango enzyme activity of serum SOD. Experimental study of using the post test control group design in which the male Wistar rats performed against. The samples were divided into five groups, with each group of 5 mice, however, over time the research during the 42 days there are dead rats especially the P3 gets a dose of 1.6 g of flour so that the remaining dead all K (-) 5 tail, K (+) 5 tail, P1 dose 0.4 g flour 4 tail, and P2 0.8 g dose 3 tail. Mango's skin flour manalagi interventions made by way of drying in an oven temperature of 100 ° C for 3 hours. The variable measured is enzyme activity of serum SOD with the method spektofotometri. Lab results obtained average serum SOD enzyme activity, i.e. K (-) 27.378 U/mL, K (+) 25.778 U/mL U/mL 26.278 P1, and P2 25.630 U/mL. Based on the test results one way ANOVA, obtained as a result p = 0,623 (p > 0.05) indicating that the absence of differences of serum SOD enzyme activity data in the four treatment groups or the results are not significant. Where the average serum SOD enzyme activities on the P2 approach K (-) which could be due to the presence of prooxidation polyphenol.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/243/051807270 |
| Uncontrolled Keywords: | diet tinggi lemak, antioksidan polifenol, aktivitas enzim SOD serum, tepung kulit mangga manalagi-Hight fat diet, polyphenols antioxidant, serum SOD enzyme activity, manalagi mango peels powder |
| Subjects: | 600 Technology (Applied sciences) > 641 Food and drink > 641.3 Food > 641.35 Spesific food from plant crops |
| Divisions: | Fakultas Kedokteran > Ilmu Gizi |
| Depositing User: | soegeng Moelyono |
| Date Deposited: | 04 May 2020 14:14 |
| Last Modified: | 21 Oct 2021 08:02 |
| URI: | http://repository.ub.ac.id/id/eprint/167341 |
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