Permatasari, Regina Safitri (2018) Pengaruh Pemberian Bawang Hitam Terhadap Kadar Mda Serum Pada Tikus Putih (Rattus Norvegicus Strain Wistar) Jantan Yang Diberi Diet Tinggi Lemak Dan Fruktosa. Sarjana thesis, Universitas Brawijaya.
Abstract
Salah satu karakteristik sindrom metabolik adalah dislipidemia. Dislipidemia mengakibatkan terjadinya proses peroksidasi lipid yang dapat menghasilkan senyawa malondialdehid (MDA). Kandungan s-allysysteine, polifenol dan flavonoid pada bawang hitam dapat menekan peroksidasi lipid sehingga dapat mencegah kadar MDA. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian bawang hitam terhadap kadar MDA pada tikus putih (Rattus norvegicus Strain Wistar) jantan yang diberi diet tinggi lemak dan tinggi fruktosa. Penelitian ini menggunakan metode true experimental dengan rancangan post test control group design. Penelitian ini menggunakan 25 ekor tikus dibagi menjadi 5 kelompok, kelompok negatif (K1) diberi diet normal + aquades, kelompok positif (K2) diberi diet normal + sonde DTLF + aquades, kelompok P1 diberi diet normal + sonde DTLF + sonde bawang hitam dosis 240 mg, kelompok P2 diberi diet normal + sonde DTLF + sonde bawang hitam dosis 480 mg serta kelompok P3 diberi diet normal + sonde DTLF + sonde bawang hitam dosis 960 mg. Kadar MDA serum diukur menggunakan metode thiobarbituric acid- reactive substance (TBARS). Berdasarkan hasil analisis data menggunakan uji One-Way ANOVA, kelompok K1 288 ± 12,82 ng/mL, K2 314 ± 18,62 ng/mL, P1(323 ± 25,51 ng/mL, P2 313 ± 24,41 ng/mL, P3 331 ± 38,36 ng/mL dengan nilai p=0,120 (p<0,05). Kesimpulan dari penelitian ini, dosis bawang putih hitam selama 14 hari belum mampu mencegah peningkatan kadar MDA serum.
English Abstract
One of the characteristics of metabolic syndrome is dyslipidemia. Dyslipidemia results in a lipid peroxidation process that can produce MDA compounds. The content of s-allycysteine, polyphenols and flavonoids in black garlic can suppress lipid peroxidation so as to prevent MDA levels. The purpose of this study was to determine the effect of black garlic on MDA levels in white rats (Rattus norvegicus Strain Wistar) males fed with high fat and fructose diet. This study was a true experimental study with post test control group design. This study used 25 rats divided into 5 groups, negative group (K1) fed with normal diet + aquades feeding tube, positive group (K2) fed with normal diet + HFFD feeding tube + aquades feeding tube, P1 group fed with normal diet + HFFD feeding tube + black garlic feeding tube dose 240 mg , P2 group fed with normal diet + HFFD feeding tube + black garlic feeding tube dose 480 mg and group P3 fed with normal diet + HFFD feeding tube + black garlic feeding tube dose 960 mg. Serum MDA levels were measured using thiobarbituric acid-reactive substance (TBARS) method. Based on data analysis using One-Way ANOVA test, K1 group was 288 ± 12.82 ng / mL, K2 314 ± 18.62 ng / mL, P1 323 ± 25.51 ng / mL, P2 313 ± 24.41 ng / mL, P3 331 ± 38 , 36 ng / mL. In conclusion, dosing of black garlic for 14 days has not been able to prevent elevated MDA serum levels.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/237/051807264 |
| Uncontrolled Keywords: | bawang hitam, kadar MDA serum, diet tinggi lemak dan fruktosa*-black garlic, MDA, high fat and fructose diet (HFFD) |
| Subjects: | 600 Technology (Applied sciences) > 641 Food and drink > 641.3 Food > 641.35 Spesific food from plant crops |
| Divisions: | Fakultas Kedokteran > Ilmu Gizi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 02 May 2020 19:06 |
| Last Modified: | 21 Oct 2021 07:33 |
| URI: | http://repository.ub.ac.id/id/eprint/167335 |
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