Montolalu, Dionisius Bryan (2018) Evaluasi Pertumbuhan Isolat Probiotik (Lactobacillus Casei Dan Lactobacillus Plantarum) Dalam Medium Susu Skim Dengan Penambahan Rumput Laut (Eucheuma Cottonii. Sarjana thesis, Universitas Brawijaya.
Abstract
Rumput laut jenis Eucheuma cottonii mengandung serat pangan (dietary fiber) yang menguntungkan bagi kesehatan manusia, dimana serat dapat berfungsi sebagai prebiotik. Dengan adanya prebiotik, probiotik dapat membantu mengeliminiasi antigen yang masuk bersama makanan. Penambahan Eucheuma cottonii pada medium susu skim, diduga mampu meningkatkan pertumbuhan probiotik (Lactobacillus casei dan Lactobacillus plantarum). Penelitian ini bertujuan untuk mengetahui pengaruh penambahan rumput laut dalam medium fermentasi susu skim terhadap pertumbuhan probiotik. Penelitian ini dilakukan menggunakan Rancangan Acak Kelompok yang disusun secara faktorial (RAKF) dengan dua faktor. Faktor I adalah medium susu skim dengan penambahan rumput laut (4 level: 10:0; 10:0,5; 10:1,0; dan 10:1,5) dalam persen. Faktor II adalah jenis isolat probiotik yang digunakan (2 jenis: Lactobacillus casei dan Lactobacillus plantarum). Hasil analisa diuji sidik ragamnya (ANOVA) dengan selang kepercayaan 95% menggunakan aplikasi pengolahan data Minitab 16. Apabila hasil uji menunjukkan beda nyata, maka dilakukan uji lanjut DMRT (Duncan Multiple Range Test) atau uji BNT (Beda Nyata Terkecil) menggunakan Microsoft Excel 2013. Perlakuan terbaik ditentukan dengan menggunakan metode Multiple Attribute (Zelleny, 1982). Hasil penelitian menunjukkan bahwa perlakuan penambahan rumput laut pada medium susu skim berpengaruh terhadap tingkat viabilitas probiotik (L. casei dan L. plantarum). Jumlah sel probiotik cenderung mengalami peningkatan seiring meningkatnya jumlah rumput laut yang ditambahkan. Perlakuan terbaik diperoleh pada penambahan rumput laut sebanyak 1,50% pada medium susu skim dengan penambahan isolat L. plantarum. Dengan peningkatan total BAL sebesar 1,15 x 107 cfu/ml, peningkatan total asam sebesar 0,39% dan penurunan pH sebesar 2,53.
English Abstract
Eucheuma cottonii seaweed contains dietary fiber which is beneficial for human health, due to fiber can be functioned as prebiotic. The presence of prebiotics can help eliminate antigens entering the body with foods. The addition of Eucheuma cottonii to skim milk medium is expected to be able to increase the growth of probiotics (Lactobacillus casei and Lactobacillus plantarum). This study aims to determine the effect of the addition of seaweed in skim milk fermentation medium to the growth of probiotics. This study was conducted using Factorial Randomized Block Design with two factors. The first factor is skim milk medium with the addition of seaweed (4 levels: 10: 0; 10: 0.5; 10: 1.0; and 10: 1.5) in percent. The second factor is the type of probiotic isolate used (2 types: Lactobacillus casei and Lactobacillus plantarum). The results of the research were tested using analysis of variance (ANOVA) with a 95% confidence interval using the Minitab 16 data processing application. If the test results showed significant differences, it will be continued with DMRT (Duncan Multiple Range Test) or BNT test (Smallest Real Difference) using Microsoft Excel 2013. The best treatment is determined using the Multiple Attribute Method (Zeleny, 1982). The results showed that the addition of seaweed to the medium of skim milk affected the level of probiotic viability (L. casei and L. plantarum). The number of probiotic cells tended to increase as the amount of seaweed is added. The best treatment was obtained by adding 1.50% seaweed in skim milk medium with the addition of L. plantarum isolates. With a total increase in LAB of 1.15 x 107 cfu / ml, increase of total acid by 0.39% and decrease in pH by 2.53.
Item Type: | Thesis (Sarjana) |
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Identification Number: | SKR/FTP/2018/538/051810881 |
Uncontrolled Keywords: | L. casei, L. plantarum, Susu Skim, Rumput Laut,/ L. casei, L. plantarum , Seaweed, Skim Milik |
Subjects: | 600 Technology (Applied sciences) > 641 Food and drink > 641.3 Food > 641.37 Dairy and related products |
Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
Depositing User: | Endang Susworini |
Date Deposited: | 02 Oct 2019 02:25 |
Last Modified: | 06 Jul 2022 04:39 |
URI: | http://repository.ub.ac.id/id/eprint/165040 |
Text
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