Putri, Shella Novia Maharani (2017) Karakteristik Daun Alga Coklat Sargassum Echinocarpum Dalam Bentuk Kering Dan Teh Dari Perairan Pulau Talango, Sumenep, Madura. Sarjana thesis, Universitas Brawijaya.
Abstract
Daun alga coklat Sargassum echinocarpum dalam bentuk kering dan teh memiliki karakteristik yang berbeda. Tujuan penelitian ini untukmengetahui karakteristik daun alga coklat Sargassum echinocarpum dalam bentuk kering dan teh yang dibandingkan dengan standar kualitas teh berdasarkan SNI (3836:2013). Sargassum echinocarpum yaitu diangin-anginkan (suhu 37ºC, 48 jam, RH 70%) juga dalam bentuk teh, direndam Ca(OH)2, pH 11, selama 6 jam dan dikeringkan oven suhu 80oC, 20 menit). Produk ini kemudian dikarakterisasi senyawa bioaktif teh (kuersetin) menggunakan LC-MS, FTIR, aktivitas antioksidan, kandungan protein sebagai bahan makanan. Sebagai produk teh, hasil penelitian menunjukkan bahwa kadar polifenol (19.54 mgGAE/100gr), serat kasar (14,24%), logam berat (kadmium (0,026 ppm), timbal (0,001 ppm), timah (0,084 ppm), merkuri dan arsen (tidak terdeteksi), dan kapang tidak terdapat kapang, memenuhi Standar Nasional Indonesia namun tidak pada kandungan air (11.07%), abu (23.23%), dan standar mikrobiologi (coliform 1,1x105 AMP/g dan TPC 21x104 koloni/g). Antioksidan IC50 sebesar 251.58 ppm, hasil FTIR teh diduga terdapat ikatan gugus hidroksil (OH), CH alifatik, C=C aromatik dan C-O mengindikasikan senyawa golongan flavonoid, dari hasil LC-MS terdeteksi kuersetin dalam kandungan yang cukup kecil. Sebagai bahan pangan memiliki protein 6.22%. Sebagai produk kering, penelitian tersebut menunjukkan bahwa kadar polifenol (11.59mgGAE/100gr),serat kasar (14.93%), logam berat (kadmium (0,023 ppm), timbal (0,001 ppm), timah (0,098 ppm), merkuri dan arsen (tidak terdeteksi), dan kapang (6 x 102) memenuhi Standar Nasional Indonesia namun tidak pada kandungan air (12.35%), abu (15.43%), dan standar mikrobiologi (coliform 1,1x105 AMP/g dan TPC 61x104 koloni/g). Antioksidan IC50 sebesar 228.21 ppm, hasil FTIR kering menunjukkan bahwa mengandung komponen sulfur dan halogen, dari hasil LC-MS terdeteksi kuersetin dalam kandngan yang cukup kecil. Sebagai bahan pangan memiliki protein 6.49%.
English Abstract
Brown algae leaf Sargassum echinocarpum in dry and tea form has different characteristics. The purpose of this research was know the characteristic of dry and tea brown algae leaves of Sargassum echinocarpum compared to tea quality standard based on Indonesian National Product Standards number (3836: 2013). Sargassum echinocarpum was dried in dry air (temperature 37ºC, 48 hours, RH 70%) as well on tea, except soaked in Ca(OH)2, pH 11, 6 hours then oven dried 80ºC, 20 minutes. These products then to be characteristation in tea quality bioactive compound (quercetin) using LCMS, FTIR, antioxidant activity, protein content as food ingredient. As tea product the research showed that polyphenol contennt (19.54 mgGAE/100gr), crude fiber (14,24%), heavy metals (cadmium (0.026 ppm), lead (0.084 ppm), tin (0.001 ppm), mercury and arsenic (undetectable)) value, mold content no detection were fulfill with Indonesian National Product Standards but not in water (11.07%, ash (23.23%, and microbiology standard (coliform (1.1x105 APM/g) and TPC (21x104 koloni/g)). IC50 of antioxidant were 251.58 ppm, from FTIR it showed group hydroxyl (OH), CH aliphatic, C=C aromatic and C-O that indicated group of flavonoid compound, from LC-MS it showed quercetin in small enough content. As food ingredient, it had 6.22% of proteins. As dry product the research showed that polyphenol contennt (11.59 mgGAE/100gr), fiber (14.93%) heavy metals (cadmium (0.023 ppm), lead (0.098ppm), tin (0.001 ppm), mercury and arsenic (undetectable)), and mold content (6 x 102) were fulfill with Indonesian National Product Standards but not in water (12.35%), ash (15.43%), and microbiology standard (coliform (1.1x105 APM/g) and TPC (61x104 koloni/g)). IC50 of antioxidant were 228.21 ppm, from FTIR it showed that contains with sulfur components and halogen, from LC-MS it showed quercetin in small enough content. As food ingredient, it had 6.49% of protein.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FPR/2017/927/051712292 |
| Uncontrolled Keywords: | Sargassum echinocarpum, Teh,Antioksidan, Kuersetin,FTIR, Sargassum echinocarpum, Tea,Antioxidant, Content quercetin, FTIR, Protein |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.8 Algae / Algae culture / Microalgae--Cultures and culture media > 579.88 Phaeophyta (Brown algae) > 579.888 Sargassum |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Manajemen Sumberdaya Perairan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 20 Dec 2017 02:01 |
| Last Modified: | 29 Sep 2020 07:54 |
| URI: | http://repository.ub.ac.id/id/eprint/7258 |
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