Gayatri, Putu Pranasari (2019) Aktivitas Antioksidan dan Antibakteri Ekstrak Fukoidan Sargassum cristaefolium dengan Metode Ultrasonic Assisted Extraction (UAE). Sarjana thesis, Universitas Brawijaya.
Abstract
Sargassum cristaefolium merupakan salah satu alga cokelat Indonesia yang hingga kini pemanfaatannya terbatas dengan harga jual Rp. 1.500- 3.000/kg. Padahal alga coklat mengandung senyawa seperti fukoidan yang berpotensi untuk dikembangkan dan menambah nilai jual. Fukoidan didapatkan dengan cara ekstraksi. Metode ekstraksi ultrasonik (UAE) dan pelarut HCl diharapkan dapat meningkatkan jumlah rendemen fukoidan. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi HCl serta waktu UAE terhadap rendemen, aktivitas antioksidan dan antibakteri. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok Faktorial (RAKF) dengan 2 faktor: konsentrasi HCl (0,01M, 0,03M, 0,05M) dan waktu UAE (10, 15, 20 menit). Hasil penelitian menunjukkan interaksi antara konsentrasi HCl dan waktu UAE berpengaruh nyata. Rendemen tertinggi pada ekstrak HCl 0.03 M waktu 15 menit sebesar 2,03±0,15%, aktivitas antioksidan tertinggi pada ekstrak HCl 0.03 M waktu 20 menit dengan nilai IC50 sebesar 65,95 ± 1,75 ppm, aktivitas antibakteri terhadap S.aureus dan E.coli tertinggi pada perlakuan HCl 0.03 M waktu 15 menit sebesar 2.73±0,08 mm dan 2,50±0,18 mm.
English Abstract
Sargassum cristaefolium is one of the Indonesian brown algae which that until now has limited use with a selling price of Rp. 1,500-3,000 / kg. In fact, brown algae contain compounds such as fucoidan which have the potential to be developed and add selling points. Fucoidan is obtained by extraction. Ultrasonic assisted extraction (UAE) method and HCl solvent are expected to increase the amount of fukoidan yield. This study aims to determine the effect of HCl concentration and UAE time on yield, antioxidant and antibacterial activity. The research design used was a Factorial Randomized Block Design (RCBD) with 2 factors: HCl concentration (0.01M, 0.03M, 0.05M) and UAE time (10, 15, 20 minutes). The results showed a significant effect between HCl concentration and UAE time. The highest yield of 0.03 M HCl extract for 15 minutes was 2.03 ± 0.15%, the highest antioxidant activity was 0.03 M for HCl extract for 20 minutes with IC50 value of 65.95 ± 1.75 ppm, antibacterial activity against S. aureus and E.coli at the highest HCl 0.03 M treatment for 15 minutes was 2.73 ± 0.08 mm and 2.50 ± 0.18 mm.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2019/630/052002496 |
| Uncontrolled Keywords: | Bioaktivitas, Fukoidan, Sargassum cristaefolium, Ultrasonic Assisted Extraction (UAE)- Bioactivity, Fucoidan, Sargassum cristaefolium, Ultrasonic Assisted Extraction (UAE) |
| Subjects: | 600 Technology (Applied sciences) > 664 Food technology > 664.02 Processes > 664.022 Extraction |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | Agus Wicaksono |
| Date Deposited: | 10 Aug 2020 07:00 |
| Last Modified: | 10 Aug 2020 07:00 |
| URI: | http://repository.ub.ac.id/id/eprint/180321 |
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