Prakoso, Adrian Bagus (2019) Pengaruh Suplementasi Bakteri Asam Laktat Terhadap Ekspresi Interleukin- 1Il-1) Serta Histopatologi Ileum Pada Tikus (Rattus Norvegicus) Yang Diinduksi Aflatoksin B1. Sarjana thesis, Universitas Brawijaya.
Abstract
Aflatoksin B1 dikenal sebagai mycotoxin paling kuat dari berbagai jenis aflatoksin yang ada.Komponen Aflatoksin B1 dapat memicu sitokin pro inflamatori untuk mengaktifkan sel imun sehingga menyebabkan kerusakan organ pencernaan.Bakteri asam laktatmempunyai kemampuan mengikat aflatoksin B1, baik secara in vitro maupun in vivo. Penelitian ini bertujuan untuk mengetahui efek suplementasi Lactobacillus bulgaricus dalam menurunkan level ekspresi IL-1 serta histopatologi ileum tikus yang diinduksi aflatoksin B1. Penelitian ini bersifat eksperimental dengan desain post test only control group menggunakan Rancangan Acak Lengkap (RAL).Hewan model menggunakan tikus putih (Rattus norvegicus) strain Wistar jantan dengan umur 8 – 10 minggu dan berat badan 150 – 250 g yang dibagi dalam lima kelompok perlakuan. Perbedaan antara kelompok negatif dengan positif yaitu diberi 7,5 µg/200 g BB dalam 1 ml PBS secara peroral selama 21 hari, serta perlakuan 1, 2, dan 3 yang disuplementasi BAL dosis 1 ml dengan konsentrasi masingmasing 1x105, 1x107, dan 1x109 CFU/mlsecara peroral selama 21 hari serta induksi AFB1 dengan dosis 7,5 µg/200 g BB dalam 1 ml PBS secara peroral mulai hari ke 8 – 28. Ekspresi IL-1 diukur secara kuantitatif menggunakan imunohistokimia serta dianalisis statistika dengan uji sidik ragam One-Way ANOVA, α = 5%. Sementara hasil pengamatan histopatologi ileum berdasarkan gambaran lapisan epitelial pada lamina propia yangdianalisa secara deskriptif. Hasil analisis statistik menunjukkan bahwa pemberian suplementasi bakteri asam laktat pada tikus putih yang diinduksi Aflatoksin B1 dengan konsentrasi 1x107 CFU/ml memberikan pengaruh sangat nyata (p<0.01) dalam menghambat peningkatan IL-1 dan dapat menghambat kerusakan jaringan ileum
English Abstract
Aflatoxin B1 had been known as the strongest mycotoxin within all aflatoxin species. Aflatoxin B1 attacks sitokin pro inflamtory to actived imunity cell could cause damage digestive system. Some researches had been done to prove the ability of Lactobacillus bulgaricuson prevent fungi growth and aflatoxin B1 binding onin vitroandin vivo. The aim of this research is to know the supplementation effect of lactic acid bacteria to reduce the expression of Interleukin-1(IL-1 and liver histopathologyof rat after AFB1 induction. This research is experimentaly with post test only control groupdesign use Completely Randomized Design.The research would usemale Winstar rat (Rattus norvegicus) with the age between 8 – 10 weeksand 150 – 250 gin weights which divided into five groups. Negative group, positive which would be given 7,5 µg/200 g BW in 1 ml PBS for 21 days, andgroup 1, 2, and 3 which would be given1 ml lactic acid bacteriawith each concentrations are 1x105, 1x107, and 1x109 CFU/mlfor 21 daysand7,5 µg/200 g BB AFB1 induction in 1 ml PBS started from day 8 – 21. Expression of Interleukin-1 decreased would be quantitatively measured by imunohistocyhemical and statistically analized with One-Way ANOVA, α = 5%. The histopathology observation of epithelial damage in the lamina propia on ileum histopathology would be descriptively analized with microscope. The results of the analysis statistics show that the suplementasted lactic acid bacteria on mice white aflatoksin b1 induced by concentration of the 1x107 cfu/ml impact open (p<0.01) increase and hinder tissue damage the ileum
Other obstract
-
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/157/051909095 |
| Uncontrolled Keywords: | Aflatoksin B1, Lactobacillus bulgaricus, Rattus norvegicus, IL-1, Histopatologi ileum, Aflatoxin B1, Lactobacillus bulgaricus, Rattus norvegicus, IL-1, Histopathology |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.085 Feeds and applied nutrition > 636.085 2 Applied nutrition |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 06:04 |
| Last Modified: | 21 Oct 2021 06:21 |
| URI: | http://repository.ub.ac.id/id/eprint/176772 |
Preview |
Text
ADRIAN BAGUS PRAKOSO (2).pdf Download (2MB) | Preview |
Actions (login required)
 |
View Item |