Utami, Sartika Dewi (2017) Pengaruh Pemberian Catechins Terhadap Ekspresi Tnf-Α Dan Apoptosis Jaringan Serta Status Fungsional Otak Tikus Jantan Galur Wistar (Rattus Norvegicus) Model Cedera Otak Traumatik Fokal. Magister thesis, Universitas Brawijaya.
Abstract
Cedera otak traumatik masih merupakan menjadi salah satu penyebab utama kecacatan, kematian dan beban ekonomi pada masyarakat. Saat ini, telah diketahui bahwa tidak semua kerusakan otak terjadi saat kejadian trauma, namun lebih banyak terjadi saat gangguan otak sekunder, yang berlangsung hingga berhari-hari, dimana proses dasarnya adalah oksidasi dan inflamasi. Catechins memiliki aktivitas antioksidan dan antiinflamasi Pada penelitian ini catechins diharapkan mampu menurunkan ekspresi TNF-α dengan menurunkan ekspresi gen STAT-1 dan melalui hambatan pada NFKβ. Serta menurunkan apoptosis sel neuron, dengan menghambat pembentukan radikal bebas melalui hambatan jalur NADPH oksidase, menetralisir radikal bebas yang telah terbentuk, serta meningkatkan protein antiapoptosis (Bcl-2) dan menurunkan protein sel proapoptosis (Bax, Bcl-XS), dan memperbaiki status fungsional otak tikus model cedera otak traumatic. Penelitian dilakukan dengan tujuan untuk mengetahui pengaruh pemberian catechins terhadap ekspresi TNF-α, sel apoptosis dan status fungsional otak tikus model cedera otak traumatik. Penelitian ini menggunakan desain eksperimental sebenarnya (true experimental design) di laboratorium secara in vivo dengan Randomized Post Test Only Controlled Group Design pada hewan model tikus wistar (Rattus novergicus galur wistar). Penelitian ini menggunakan hewan coba sebanyak 40 ekor yang terbagi dalam 2 kelompok waktu pengamatan, masing-masing terdiri dari 5 kelompok perlakuan, tiap kelompok terdiri dari 4 ekor tikus. Kelima kelompok tersebut adalah kelompok A (kontrol negatif) yaitu tikus yang tidak diberikan perlakuan cedera otak traumatik maupun catechins, kelompok B (kontrol positif) yaitu model cedera otak traumatik tanpa diberi catechins, kelompok C, D, dan E yaitu kelompok perlakuan yang menjadi model cedera otak traumatik dan diberi catechins dengan dosis 513, 926, dan 1113 mg/kgbb/hari. Pemberian catechins diberikan secara peroral dan pengamatan ekspresi TNF-α, apoptosis sel dan status fungsional otak tikus dilakukan setelah perlakuan selama tiga dan tujuh hari. Berdasarkan hasil uji beda ANOVA dan Kruskall wallis yang dilakukan untuk mengetahui pengaruh pemberian catechins terhadap ekspresi TNF-α, apoptosis sel dan status fungsional otak, didapatkan hasil secara berurutan pada hari ketiga (p=0.002, p=0.004, p=0.000; p<0.05) dan pada hari ketujuh (p=0.000, p=0.000, p=0.002; p<0.05) maka terdapat perbedaan yang nyata antar kelompok pada semua variable dan waktu pengamatan. Hasil uji korelasi Spearman dan Pearson, menunjukkan nilai r dan p secara berurutan pada hari ketiga (r= -0.838, p=0.000; r= -0.691, p=0.003; R= -0.441, p=0.088) dan pada hari ketujuh (r= -0.921, p=0.000; r=-0.890, p=0.000; r=-0.754, p=0.001) menunjukkan bahwa terdapat hubungan bermakna antara dosis catechins dan sel apoptosis, semakin tinggi dosis catechins makin rendah ix sel apoptosis kecuali NSS pada hari 3, terdapat hubungan yang signifikan antara dosis pemberian catechins dengan ekspresi TNF-α, apoptosis sel dan status fungsional otak tikus. Hasil uji perbandingan berpasangan pada NSS, menunjukkan p=0.006 (hari 3) dan p=0.003 (hari 7), menunjukkan adanya perbedaan yang signifikan antara sebelum dan sesudah pemberian catechins. Penelitian ini menyimpulkan bahwa pemberian catechins dapat menurunkan ekspresi TNF-α dan apoptosis sel serta meningkatkan status fungsional otak tikus, serta terdapat hubungan yang signifikan antara peningkatan dosis catechins dengan penurunan ekspresi ekspresi TNF-α dan apoptosis sel serta meningkatkan status fungsional otak tikus wistar (Rattus norvegicus) model cedera otak traumatik fokal.
English Abstract
Traumatic brain injury is still one of the major causes of disability, death and economic burden on people. Currently, it is known that not all brain damage occurs during trauma events, but more often occurs during secondary brain disorders, which last for days, where the underlying process is oxidation and inflammation. Catechins have antioxidant and anti-inflammatory activity In this study catechins are expected to decrease TNF-α expression by decreasing STAT-1 gene expression and through resistance to NFKβ. As well as decrease the apoptosis of neuron cells, by inhibiting the formation of free radicals through blockage of NADPH oxidase pathways, neutralizing the free radicals that have been formed, as well as increasing the antiapoptosis protein (Bcl-2) and lowering proapoptotic cell proteins (Bax, Bcl-XS), and improving functional status rat brain traumatic brain injury model. The aim of this study was to investigate the effect of catechins on TNF-α expression, apoptotic cells and functional status of rat brain of traumatic brain injury model. This study used true experimental design in laboratory in vivo with Randomized Post Test Only Controlled Group Design on animal model of wistar rat (Rattus novergicus wistar strain). This study used 40 rats of experimental divided into 2 groups of observation time, each consisting of 5 interventional groups, each group consisting of 4 rats. The five groups were group A (negative control), rat that were not given traumatic brain injury treatment or catechins, group B (positive control), traumatic brain injury model without given catechins, group C, D, and E, traumatic brain injury and given catechins with doses of 513, 926, and 1113 mg / kgbody weight / day. Administering catechins administered peroralally and TNF-α expression observations, cell apoptosis and functional status of rat brain were performed after three and seven days of treatment. Based on the different ANOVA and Kruskall wallis test results to determine the effect of catechins on TNF-α expression, cell apoptosis and functional status of the brain, the results obtained sequentially on the third day (p = 0.002, p = 0.004, p = 0.000; p < 0.05) and on the seventh day (p = 0.000, p = 0.000, p = 0.002; p <0.05), there were significant differences between groups in all variables and observation time. Spearman and Pearson correlation test results show r and p values respectively on the third day (r = -0.838, p = 0.000; r = -0.691, p = 0.003; r = -0.441, p = 0.088) and on the seventh day r = -0.921, p = 0.000, r = -0.890, p = 0.000, r = -0.754, p = 0.001) showed that there was a significant association between dosage of catechins and apoptotic cells, the higher the dosage of catechins the lower the apoptotic cells except NSS on day 3, there was a significant relationship between the dose of administration of catechins with TNF-α expression, cell apoptosis and functional status of rat brain. The result of paired comparison test on NSS, showed p = 0.006 (day 3) and p = 0.003 (day 7), showed significant difference between before and after catechins administration. The present study concluded that administration of catechins decreased TNF-α expression and cell apoptosis and improved functional status of rat brain, and there was a significant relationship between increased doses of catechins with decreased expression of TNF-α expression and cell apoptosis and improved functional status of the brains of wistar rats (Rattus norvegicus) model of focal traumatic brain injury
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.323 624/UTA/p/2017/041712453 |
| Uncontrolled Keywords: | TEA - HEALTH ASPECTS, TEA - THERAPENTIC USE, CATECHIN, BRAIN |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 624 Drugs derived from specific plants (Mangosteens, Tea) |
| Divisions: | S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran |
| Depositing User: | Nur Cholis |
| Date Deposited: | 17 Apr 2018 06:22 |
| Last Modified: | 25 Nov 2021 06:46 |
| URI: | http://repository.ub.ac.id/id/eprint/9534 |
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