Karoma, Denis Al (2018) Pengaruh Penggunaan Pelarut Metanol dan Diklorometana dalam Derivatisasi Sitronelal menjadi Benzimidazol dan Uji Aktivitasnya sebagai Antibakteri. Sarjana thesis, Universitas Brawijaya.
Abstract
Benzimidazol merupakan senyawa yang memiliki bioaktivitas sangat luas, seperti antibakteri, antiinflamasi dan antianalgesik. Senyawa ini dapat diperoleh dari reaksi kondensasi senyawa aldehid dengan o-fenildiamin. Dalam penelitian ini, benzimidazol disintesis dari sitronelal dalam minyak jeruk purut dengan menggunakan pelarut metanol dan diklorometana dengan metode microwave. Karakterisasi benzimidazol hasil sintesis dilakukan dengan spektrofotometer UV-VIS, FTIR, KGSM dan H-NMR. Sedangkan untuk uji aktivitas antibakteri penentuan zona hambat dan konsentrasi hambat minimum dengan metode difusi cakram dan microdilution yang dilakukan terhadap bakteri Staphylococcus aureus, Salmonella thypi dan EPEC. Berdasarkan hasil sintesis benzimidazol diperoleh waktu reaksi optimum dengan pelarut diklorometana 60 menit, pelarut metanol 50 menit dengan % yield berturut-turut adalah (17.2%) dan (17.8%). Karakter spektrum UV-VIS benzimidazol memiliki λmax 267 nm, pita spesifik spektrum FTIR meliputi gugus imina C=N (1630 cm-1) dan N-H amina sekunder (3300 cm-1). Spektra massa benzimidazol memiliki ion molekuler m/z 242 dan ion fragmen puncak 145, sedangkan karakter H-NMR terdiri atas 22 proton dengan sinyal spesifik benzyl pada δ= 7 ppm, indole pada δ=3 ppm dan alkil pada δ=0.9 ppm. Uji aktivitas antibakteri benzimidazol terhadap bakteri S.aureus, S.thypi dan EPEC lebih kuat dibandingkan dengan minyak jeruk purut, masing-masing memiliki zona hambat dan KHM berturut turut 8.3 mm ; 99.84%, 10,7 mm ; 99.85% dan 12 mm; 98.69%.
English Abstract
Benzimidazole is a compound that has a very wide bioactivity, such as antibacterial, anti-inflammatory, and antianalgesic. This compound can be obtained from the condensation reaction of the aldehyde compound with o-phenylenediamine. In this study, benzimidazole was synthesized from citronellal in kaffir lime oil using metanol and dichloromethane solvent by microwave method. Characterization of benzimidazole from synthesis was done by UV-VIS spectrophotometer, FTIR, KGSM and H-NMR. While for antibacterial activity of inhibiting zone determination and minimum inhibitory concentration by disc diffusion method and microdilution to Staphylococcus aureus bacteria, Salmonella thypi and EPEC. Based on benzimidazole synthesis result obtained optimum reaction time with 60 minutes for dichloromethane, and methanol solvent 50 minutes with % yields were (17.2%) and (17.8%). The spectrum character of UV-VIS benzimidazole has λmax 267 nm, the specific band of the FTIR spectrum includes the imine group C = N (1630 cm-1) and the secondary N-H (3300 cm-1). The mass spectra of benzimidazole have molecular ions m/z 242 and peak fragment ions 145, while the H-NMR character consists of 22 protons with a specific signal of benzyl at δ = 7 ppm, indole at δ = 3 ppm and alkyl at δ = 0.9 ppm. The antibacterial activity test of benzimidazole to S.aureus, S.thypi and EPEC bacteria was stronger than the kaffir lime oil, each having inhibitory zone and KHM respectively 8.3 mm; 99.84%, 10,7 mm; 99.85% and 12 mm; 98.69%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/543/051800341 |
| Uncontrolled Keywords: | sitronelal, imina, antibakteri, metanol, diklorometana, citronellal, imine, antibacterial, methanol, dichlorometane |
| Subjects: | 500 Natural sciences and mathematics > 572 Biochemistry > 572.7 Enzymes |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 01 Feb 2018 04:33 |
| Last Modified: | 22 Oct 2021 05:22 |
| URI: | http://repository.ub.ac.id/id/eprint/8662 |
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