Hartono, Budi (2017) Pengaruh Ekstrak Daun Kelor (Moringa Oleifera) Terhadap Intake Pakan Dan Bmi (Indeks Masa Tubuh) Pada Ikan Zebra (Danio Rerio) Model Dio (Diet Induced Obesity. Sarjana thesis, Universitas Brawijaya.
Abstract
Obesitas merupakan masalah kesehatan yang banyak diderita oleh manusia di seluruh dunia yang memicu berbagai komplikasi, diantaranya diabetes melitus tipe 2, hiperlipidemia, dan aterosklerosis. Kelor (Moringa oliefera) memiliki beberapa kandungan yang bersifat antiobesitas pada tikus dan kultur sel preadiposit. Zebrafish merupakan alternatif dalam meneliti aktivitas bahan alam dan DIO. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak daun kelor (Moringa oleifera) terhadap Intake pakan dan BMI pada zebrafish yang diberi DIO (Diet Induced Obesity). Studi eksperimental labolatorik ini menggunakan pre–post test control group design dengan sampel zebrafish betina usia 5 mpf (month post fertilization). Pemilihan sampel dilakukan dengan simple random sampling. Metode induksi obesitas yang digunakan adalah pemberian makan berlebih dengan Artemia. Sampel dibagi menjadi lima kelompok yang masing-masing terdiri dari lima ekor zebrafish, pada kelompok kontrol negatif (Artemia 5 mg/hari), DIO (Artemia 60 mg/hari), Dosis A, (Artemia 60 mg/hari + Kelor 0.56 ppm, Dosis B (Artemia 60 mg/hari + kelor 1.12 ppm), dan Dosis C (Artemia 60mg/hari + kelor 2.24 ppm). Variabel yang diukur adalah BMI yang diukur pada hari ke–14, 20, 27, 34, dan 40 dan intake pakan diukur pada hari ke–39. Hasil penelitian menunjukan bahwa tidak ada beda nyata terhadap rata–rata BMI diantara kelompok DIO maupun kelompok perlakuan pada ketiga dosis (p>0.05), tetapi hanya dosis kelor 2.24 ppm yang berpengaruh terhadap intake pakan zebrafish (p<0.05). Disimpulkan, pemberian ekstrak kelor dalam berbagai dosis tidak berpengaruh terhadap BMI pada zebrafish dan dosis yang mulai berpengaruh terhadap intake pakan adalah dosis kelor 2.24ppm.
English Abstract
Obesity is a worldwide health problem that affect a lot of people and trigger a variety of complications, including type-2 diabetes mellitus, hyperlipidemia, and atherosclerosis. Kelor (Moringa oliefera) content some substance that has antiobesity activity in rats and preadipocyte cell culture. Zebrafish is an alternative in conducting research of natural compounds activities and DIO. The purpose of this research is to evaluate the effect of MOLE (Moringa oliefera leaves extract) on feed intake and BMI in zebrafish with DIO (Diet Induced Obesity). This laboratory experimental study used a pre-posttest control group design with a female zebrafish sample of 5-month post fertilization (mpf). The sample selection was done by simple random sampling. The method of induction of obesity used is overfeeding with Artemia. The samples were divided into five groups consisting of five zebrafish, such as control group (Artemia 5 mg / day), DIO (Artemia 60 mg / day), Dose A (Artemia 60 mg / day + 0.56 ppm MOLE), Dose B (Artemia 60 mg / day + 1.12 ppm MOLE), and Dose C (Artemia 60mg / day + 2.24 ppm MOLE). The BMI measured at days 14, 20, 27, 34, and 40 and feed intake was measured on day 39. The results showed that there was no significant difference in BMI among DIO group or treatment group in all three doses (p> 0.05), but only 2.24 ppm had an effect on feed intake zebrafish (p <0.05). It concluded that the assignment of MOLE in various doses does not affect the BMI in zebrafish and affected the feed intake is the dose of 2.24 ppm MOLE.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2017/483/051800573 |
| Uncontrolled Keywords: | Obesitas, BMI, intake pakan, Zebrafish, Ekstrak Daun Kelor (Moringa oliefera), Obesity, BMI, food intake, Zebrafish, etanolic extract of Moringa oliefera leaf’s (EEMOL) |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.3 Diseases of digestive system > 616.39 Nutritional and metabolic diseases > 616.398 Obesity |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 23 Jan 2018 06:21 |
| Last Modified: | 25 Oct 2021 01:48 |
| URI: | http://repository.ub.ac.id/id/eprint/8348 |
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LAMPIRAN total.pdf Download (836kB) | Preview |
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Lembar Pengesahan.pdf Download (175kB) | Preview |
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Abstract.pdf Download (249kB) | Preview |
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