Timur, Hanan Dwi Laksono (2017) Isolasi Dan Identifikasi Bakteri Endofit Mangrove Sonneratia Alba Penghasil Enzim Gelatinase Dari Pantai Sendang Biru, Malang, Jawa Timur. Sarjana thesis, Universitas Brawijaya.
Abstract
Enzim gelatinase merupakan enzim yang berperan penting pada sektor industri pangan dan nonpangan. Enzim gelatinase berasal dari hewan, tumbuhan dan mikroorganisme. Enzim gelatinase termasuk enzim protease dan berfungsi sebagai pengurai gelatin. Penelitian ini bertujuan untuk mendapatkan bakteri penghasil enzim gelatinase dari endofit mangrove Sonneratia alba dan untuk mengetahui jenis spesies bakteri penghasil enzim gelatinase dari endofit mangrove Sonneratia alba dari pantai Sendang Biru. Metode penelitian yang digunakan adalah deskriptif eksploratif yang dilakukan dalam 2 tahapan. Tahap pertama yang dapat menghasilkan enzim gelatinase dari endofit mangrove Sonneratia alba yaitu isolasi dan skrining bakteri. Tahap kedua yaitu mengidentifikasi bakteri penghasil enzim gelatinase berdasarkan uji microbact system. Hasil isolasi dari endofit mangrove didapatkan tiga isolat bakteri dengan kode A.SASB, B.SASB dan D.SASB. Isolat daun Sonneratia alba menghasilkan enzim gelatinase yang baik, sehingga dilakukan uji microbact system. Bakteri endofit daun pada uji microbact system diketahui sebagai jenis bakteri Paenibacillus alvei dengan hasil oksidase negatif sehingga termasuk bakteri gram positif, sehingga dilakukan uji microbact 12A/E. Karakteristik bakteri setelah diamati di bawah mikroskop dengan perbesaran 1000x memiliki karakteristik berbentuk bulat dan berwarna ungu.
English Abstract
Gelatinase enzyme is an enzyme that plays an important role in the food and nonfood industry sector. Gelatinase enzymes can be isolated from animals, plants and microorganisms. Gelatinase enzymes is protease enzymes and act as decomposers of gelatin. The objective of this research was to obtain gelatinase enzyme bacteria from endemic morrow of Sonneratia alba and to know the type of gelatinaseproducing bacterial species from endemic mangrove of Sonneratia alba from Sendang Biru beach. The research method used descriptive explorative done in two stages. The first stage is isolation of microorganisme that can produce gelatinase enzyme from endophytes of Sonneratia alba mangroves. The second stage is to identify gelatinase-producing bacteria based on microbact system test. The result of isolation from mangrove endophyte obtained three isolates of bacteria with a code of A.SASB, B.SASB and D.SASB. Isolate from leaves of Sonneratia alba produces good gelatinase enzyme, so the microbact system test was done. The isolate bacteria in microbact system test known as the type of bacteria Paenibacillus alvei with negative oxidase results so that including gram-positive bacteria, so the test microbact 12A / E. The bacterial characteristics after being observed under a microscope with 1000x magnification have round and purple characteristics.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FPR/2017/1073/051800118 |
| Uncontrolled Keywords: | Enzim, Gelatinase, Sonneratia alba, Microbact System, Paenibacillus alvei, Enzyme, Gelatinase, Sonneratia alba, Microbact System, Paenibacillus alvei |
| Subjects: | 500 Natural sciences and mathematics > 572 Biochemistry > 572.7 Enzymes |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Manajemen Sumberdaya Perairan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 15 Jan 2018 03:46 |
| Last Modified: | 04 Nov 2020 02:39 |
| URI: | http://repository.ub.ac.id/id/eprint/8019 |
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