Istifada, Valia and Muhammad Akhid Syib’li, S.P., M.P., Ph.D. and Antok Wahyu Sektiono,, S.P., M.P. (2023) Uji Antagonisme Jamur Tanah dan Bakteri untuk Mengendalikan Patogen Phytophthora nicotianae pada Nanas. Sarjana thesis, Universitas Brawijaya.
Abstract
Nanas (Ananas comosus) merupakan tanaman yang memiliki potensi perdagangan yang tinggi. Dalam kegiatan produksi nanas tidak terlepas dari serangan Phytophthora nicotianae yang dapat menyebabkan kehilangan hasil hingga 100%. Upaya pengendalian Phytophthora nicotianae yang banyak dilakukan yaitu menggunakan fungisida. Penggunaan fungisida yang terus menerus dapat menyebabkan resistensi, dan resurgensi. Untuk mencegah adanya dampak tersebut, maka diperlukan pengendalian hayati menggunakan jamur dan bakteri. Tujuan penelitian ini adalah untuk mengetahui potensi jamur dan bakteri antagonis sebagai agens pengendali Phytophthora nicotianae. Penelitian dilaksanakan di Laboratorium Pengendalian Hayati 1, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya, Malang. Penelitian dilaksanakan pada bulan Oktober hingga Desember. Metode yang digunakan yaitu pengambilan sampel tanah, isolasi, purifikasi, dan identifikasi, dan uji antagonisme secara in vitro. Uji antagonis dilakukan dengan uji oposisi langsung. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) untuk delapan perlakuan termasuk kontrol dan setiap perlakuan diulang sebanyak empat kali. Pada penelitian ini diperoleh hasil uji antagonisme antara jamur antagonis dan Phytophthora nicotianae yang memiliki persentase daya hambat tertinggi adalah Penicillium sp. (PEG01) sebesar 52,68%, dan yang memiliki diameter total terendah adalah Trichoderma sp. (TRC01) sebesar 4,81 cm. Sedangkan pada uji antagonisme antara bakteri dan Phytophthora nicotianae yang memiliki persentase daya hambat tertinggi, dan diameter total terendah adalah bakteri C yaitu sebesar 78,45% dan 3,38 cm. Jamur antagonis dapat dikatakan efektif apabila persentase daya hambat yang dihasilkan tinggi dan didukung dengan rata-rata diameter totalnya rendah. Diketahui bahwa Penicillium sp. memiliki persentase daya hambat tertinggi namun Trichoderma sp. (TRC01) memiliki rata-rata diameter total terendah. Hal ini dapat disebabkan oleh kecepatan tumbuh yang berbeda. Jamur antagonis yang memiliki kecepatan tumbuh yang cepat dapat mempengaruhi diameter patogen secara horizontal sekaligus vertikal.
English Abstract
Pineapple (Ananas comosus) is a plant that has high trade potential because the demand for pineapple from within and outside the country is quite large. Pineapple production activities can't be separated from plant pathogen attacks. Phytophthora nicotianae causes heart rot in pineapples and can cause up to 100% yield loss. The most widely used control of Phytophthora nicotianae is using a fungicide with the active ingredient mancozeb. Fungicides used continuously can cause resistance and resurgence. To prevent these impacts, it is necessary to use biological control using fungi and bacteria. The purpose of this study was to determine the potential of antagonistic fungi and bacteria as controlling agents for Phytophthora nicotianae. The research was conducted at the Laboratory of Biological Control 1, Department of Plant Pests and Diseases, Faculty of Agriculture, Brawijaya University, Malang. The research was conducted from October to December. The method used is soil sampling, isolation, purification, identification, and in vitro antagonism tests. The antagonist test is done by the dual culture method. This study used a complete randomized design for eight treatments including the control and each treatment was repeated four times. In this study, the antagonism test results were obtained between antagonistic fungi and Phytophthora nicotianae which had the highest percentage of inhibition, namely Penicillium sp. (PEG01) of 52.68%, and the one with the lowest total diameter was Trichoderma sp. (TRC01) of 4.81 cm. Whereas in the antagonism test between bacteria and Phytophthora nicotianae which had the highest percentage of inhibition, and the lowest total diameter was bacteria C which were 78.45% and 3.38 cm. Antagonistic fungi can be said to be effective if the percentage of inhibition produced is high and supported by a low average total diameter. It is known that Penicillium sp. has the highest percentage of inhibition but Trichoderma sp. (TRC01) has the lowest average total diameter. This can be caused by different growth rates. Antagonistic fungi that have a fast growth rate can affect the diameter of the pathogen horizontally as well as vertically
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0523040166 |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture > 338.16 Production efficiency |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | PKN 01 UB |
| Date Deposited: | 13 Jun 2023 07:52 |
| Last Modified: | 13 Jun 2023 07:52 |
| URI: | http://repository.ub.ac.id/id/eprint/201557 |
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