Lestari, Inayah Rizki and Prof. Dr. Ir. Aminudin Afandhi, MS. (2023) Isolasi dan Identifikasi Jamur Serangga dari Tanah Pematang Sawah. Sarjana thesis, Universitas Brawijaya.
Abstract
Jamur patogen serangga merupakan agens hayati yang tersebar luas dan sering berkontribusi dalam mengatur populasi serangga secara alami. Berbagai jenis jamur serangga pada tanah dapat diperoleh menggunakan metode umpan serangga (insect bait). Jamur yang diisolasi dari serangga memiliki keanekaragaman fungsional yaitu jamur patogen serangga, patogen oportunis dan koloniser sekunder. Pematang sawah memiliki keanekaragaman vegetasi gulma yang menyediakan kondisi lingkungan yang sesuai bagi kehidupan jamur serangga. Penelitian dilaksanakan dengan mengambil sampel tanah pada pematang sawah milik petani di Desa Mulyoagung, Kecamatan Dau, Kabupaten Malang. Kegiatan dilakukan di Laboratorium Pengendalian Hayati 2 Fakultas Pertanian, Universitas Brawijaya. Penelitian dimulai pada bulan November 2022 hingga Januari 2023. Penelitian ini merupakan penelitian deskriptif dan eksperimental. Penelitian deskriptif meliputi identifikasi keanekaragaman gulma serta isolasi dan identifikasi jamur serangga dari tanah pematang sawah, sedangkan eksperimental meliputi uji patogenisitas jamur serangga terhadap larva T. molitor. Hasil penelitian pada pematang sawah ditemukan 8 jenis gulma dari 6 famili. Jenis gulma dengan jumlah individu tertinggi berasal dari keluarga Poaceae (rerumputan). Jamur serangga yang terisolasi dari tanah pematang sawah didapatkan sebanyak 22 isolat jamur yang terdiri dari 4 genus yaitu Fusarium, Aspergillus, Beauveria dan Metarhizium. Terdapat 12 isolat dari genus Fusarium, 4 isolat dari genus Aspergillus, 5 isolat dari genus Beauveria dan 1 isolat dari genus Metarhizium. Keanekaragaman fungsional jamur serangga terdiri dari 11 patogen oportunis dan 11 koloniser sekunder yang menyebabkan mortalitas pada larva T. molitor sebesar 3,33%-23,33%.
English Abstract
Insect pathogenic fungi are widespread biological agents and often contribute to the natural regulation of insect populations. Various types of insect fungi in soil can be obtained using the insect bait method. Fungi isolated from insects have functional diversity, namely insect pathogenic fungi, opportunistic pathogens and secondary colonizers. Paddy field bunds have a diversity of weed vegetation which provides suitable environmental conditions for the life of insect fungi. The research was carried out by taking soil samples in the bunds of farmers' rice fields in Mulyoagung Village, Dau District, Malang Regency. The activity was carried out at the Laboratory of Biological Control 2, Faculty of Agriculture, Brawijaya University. The research started in November 2022 until January 2023. This research is a descriptive and experimental research. The descriptive study included identification of weed diversity and the isolation and identification of insect fungi from paddy field bunds, while the experimental study included the pathogenicity test of insect fungi against T. molitor larvae. Paddy field bunds consist of 8 types of weeds from 6 families. The type of weed with the highest number of individuals comes from the Poaceae (grass) family. Insect fungi isolated from paddy field bunds obtained as many as 22 isolated fungi consisting of 4 genera, namely Fusarium, Aspergillus, Beauveria and Metarhizium. There were 12 isolates from the genus Fusarium, 4 isolates from the genus Aspergillus, 5 isolates from the genus Beauveria and 1 isolate from the genus Metarhizium. The functional diversity of insect fungi consists of 11 opportunist pathogens and 11 secondary colonizers which cause mortality in T. molitor larvae of 3.33% -23.33%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0523040081 |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture > 338.16 Production efficiency |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | PKN 01 UB |
| Date Deposited: | 07 Jun 2023 02:45 |
| Last Modified: | 07 Jun 2023 02:45 |
| URI: | http://repository.ub.ac.id/id/eprint/201031 |
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Text (DALAM MASA EMBARGO)
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