Wijayanti, Marcella Nur and Ir. Sri Lestari Purnamaningsih,, MS. and Dr. Darmawan Saptadi, SP., MP. and Taufiq Hidayat, R. S., M. Si. (2022) Invigorasi Dengan Osmoconditioning Untuk Peningkatan Viabiltas Dan Vigor Benih Kenaf (Hibiscus Cannabinus L.). Sarjana thesis, Universitas Brawijaya.
Abstract
Tanaman kenaf merupakan tanaman serat yang mempunyai potensi tinggi dalam usaha tani. Namun tanaman kenaf mengalami penurunan produktivitas. Salah satu masalah dalam penurunan produksi kenaf adalah kualitas benih yang buruk. Kualitas benih erat kaitannya dengan viabilitas dan vigor benih. Viabilitas dan vigor benih yang menurun dapat ditingkatkan dengan osmoconditioning. Tujuan dari penelitian ini adalah untuk mengetahui kombinasi perlakuan konsentrasi Kalsium Klorida (CaCl2) dan durasi perendaman yang tepat dan efisien terhadap vigor dan viabilitas benih tanaman kenaf di laboratorium dan mengetahui kombinasi perlakuan konsentrasi Kalsium Klorida (CaCl2) dan durasi perendaman yang tepat dan efisien terhadap vigor tanaman kenaf di greenhouse. Penelitian terdiri dari dua tahap. Penelitian yang pertama dilakukan di Laboratorium Benih BALLITAS Malang. Penelitian dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) satu faktor terdiri dari sepuluh taraf (P0, P1, P2, P3, P4, P5, P6, P7, P8, P9) dengan empat kali ulangan. Kombinasi perlakuan terdiri dari konsentrasi Kalsium Klorida (CaCl2) dan durasi perendaman. Satuan perlakuan yaitu: P0 (Kontrol), P1 (50 mM CaCl2 + 6 jam), P2 (50 mM CaCl2 + 12 jam), P3 (50 mM CaCl2 + 24 jam), P4 (100 mM CaCl2 + 6 jam), P5 (100 mM CaCl2 + 12 jam), P6 (100 mM CaCl2 + 24 jam), P7 (150 mM CaCl2 + 6 jam), P8 (150 mM CaCl2 + 12 jam), P9 (150 mM CaCl2 + 24 jam). Variabel pengamatan dalam uji laboratorium terdiri dari pengamatan indeks vigor, keserempakan tumbuh, kecepatan tumbuh kecambah, daya berkecambah, potensi tumbuh maksimum, panjang hipokotil, panjang radikula dan berat kering kecambah. Penelitian kedua dilakukan di greenhouse BALITTAS Malang. Penelitian dilakukan dengan menggunakan Rancangan Acak Kelompok (RAK) satu faktor terdiri dari tujuh taraf (P0, P1, P2, P4, P5, P7, P8). Tiap perlakuan diulang sebanyak empat kali ulangan. Kombinasi perlakuan terdiri dari konsentrasi Kalsium Klorida (CaCl2) dan durasi perendaman. Satuan perlakuan yaitu: P0 (Kontrol), P1 (50 mM CaCl2 + 6 jam), P2 (50 mM CaCl2 + 12 jam), P3 (50 mM CaCl2 + 24 jam), P4 (100 mM CaCl2 + 6 jam), P5 (100 mM CaCl2 + 12 jam), P6 (100 mM CaCl2 + 24 jam), P7 (150 mM CaCl2 + 6 jam), P8 (150 mM CaCl2 + 12 jam). Variabel pengamatan di greenhouse terdiri dari kecepatan tumbuh kecambah, tinggi tanaman, jumlah daun, bobot basah batang, bobot kering batang bobot basah akar, bobot kering akar, bobot basah daun, bobot kering daun. Seluruh data kuantitatif yang telah dihimpun dianalisis menggunakan uji statistika Annova One-Ways dengan menggunakan software DSAASTAT. Selanjutnya dilakukan uji lanjut dengan DMRT (Duncan’s Multiple Range Test) pada taraf 5%. Perlakuan P1 menjadi perlakuan yang efisien dalam mempengaruhi variabel pengamatan indeks vigor, keserempakan tumbuh, kecepatan tumbuh, potensi tumbuh maksimum, panjang hipokotil, panjang radikula, dan berat kering kecambah. Percobaan di greenhouse menghasilkan bahwa perlakuan P1 (CaCl2 50 mM + 6 jam) menjadi perlakuan yang paling efisien terhadap variabel pengamatan kecepatan tumbuh kecambah, tinggi tanaman, bobot basah daun, bobot kering batang, bobot kering daun namun kurang efisien terhadap variabel pengamatan bobot basah batang, bobot basah akar dan bobot kering akar.
English Abstract
Kenaf is a fiber plant that has high potential in farming. However, kenaf experienced a decrease in productivity. One of the problems in decreasing kenaf production is poor seed quality. Seed quality is closely related to seed viability and vigor. Decreased seed viability and vigor can be increased by osmoconditioning. The purpose of this study was to determine the combination of treatment with calcium chloride (CaCl2) concentration and duration of immersion that was appropriate and efficient on vigor and viability of kenaf seeds in the laboratory and to determine the combination of treatment with calcium chloride concentration (CaCl2) and duration of immersion that was appropriate and efficient on vigor. kenaf in greenhouse. The research consisted of two stages. The first research was conducted at the BALLITAS Seed Laboratory, Malang. The study was conducted using a one-factor completely randomized design (CRD) consisting of ten levels (P0, P1, P2, P3, P4, P5, P6, P7, P8, P9) with four replications. The treatment combination consisted of the concentration of Calcium Chloride (CaCl2) and the duration of immersion. The treatment units were: P0 (Control), P1 (50 mM CaCl2 + 6 hours), P2 (50 mM CaCl2 + 12 hours), P3 (50 mM CaCl2 + 24 hours), P4 (100 mM CaCl2 + 6 hours), P5 (100 mM CaCl2 + 12 hours), P6 (100 mM CaCl2 + 24 hours), P7 (150 mM CaCl2 + 6 hours), P8 (150 mM CaCl2 + 12 hours), P9 (150 mM CaCl2 + 24 hours). Variable observed in laboratory tests consisted of observations of vigor index, growth synchronously, speed of germination, germination, maximum growth potential, hypocotyl length, radicle length and sprout dry weight. The second research was conducted at the BALITTAS Malang green house. The study was conducted using a one-factor Randomized Block Design (RAK) consisting of seven levels (P0, P1, P2, P4, P5, P7, P8). Each treatment was repeated four times. The treatment combination consisted of the concentration of Calcium Chloride (CaCl2) and the duration of immersion. The treatment units were: P0 (Control), P1 (50 mM CaCl2 + 6 hours), P2 (50 mM CaCl2 + 12 hours), P3 (50 mM CaCl2 + 24 hours), P4 (100 mM CaCl2 + 6 hours), P5 (100 mM CaCl2 + 12 hours), P6 (100 mM CaCl2 + 24 hours), P7 (150 mM CaCl2 + 6 hours), P8 (150 mM CaCl2 + 12 hours). Variable observed in the greenhouse consisted of speed of germination, plant height, number of leaves, stem wet weight, stem dry weight, root wet weight, root dry weight, leaf wet weight, leaf dry weight. All quantitative data that has been collected were analyzed using the Annova One-Ways statistical test using DSAASTAT software. Furthermore, further tests were carried out with DMRT (Duncan's Multiple Range Test) at a level of 5%. The P1 treatment was an efficient treatment in influencing the observed variables of vigor index, simultaneous growth, growth speed, maximum growth potential, hypocotyl length, radicle length, and dry weight of sprouts. Experiments in the greenhouse showed that the P1 treatment (CaCl2 50 mM + 6 hours) was the most efficient treatment for the variables observed for germination speed, plant height, stem wet weight, leaf wet wight, stem dry weight, leaf dry wight, but less efficient for stem wet weight, root wet weight and root dry weight.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522040447 |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture > 338.16 Production efficiency |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 06 Apr 2023 01:48 |
| Last Modified: | 06 Apr 2023 01:48 |
| URI: | http://repository.ub.ac.id/id/eprint/198040 |
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