Widagdo, Bachtiar Dio and Dr. Ir. Budi Prasetya (2022) Pengaruh Vermikompos Terhadap Perbanyakan Mikoriza (Glomus Sp) Lahan Pasca Tambang Batu Bara Dengan Inang Tanaman Serealia. Sarjana thesis, Universitas Brawijaya.
Abstract
Glomus sp merupakan mikoriza arbuskula (MA) yang dapat ditemukan di lahan pasca tambang batu bara di Kelurahan Margomulyo, Kecamatan Samboja, Kabupaten Kutai Kartanegara, Provinsi Kalimantan Timur sebanyak 100-196 spora 100 g tanah-1 dengan kolonisasi mencapai 81%. Glomus sp berpotensi digunakan untuk membantu percepatan revegetasi lahan karena kemampuan adaptasi terhadap kondisi lingkungan asal. Kondisi tersebut bertolak belakang dengan jumlah MA yang cenderung terbatas, mudah rusak dan hilang sehingga diperlukan perbanyakan spora MA (Glomus sp) melalui kultur pot. Tanaman jagung, milet, dan sorgum dapat menjadi pilihan inang dalam perbanyakan spora MA sedangkan vermikompos dapat digunakan sebagai sumber unsur hara alternatif media perbanyakan. Tujuan penelitian ini adalah menganalisis penambahan vermikompos, pengaruh jagung, milet dan sorgum sebagai tanaman inang, dan kombinasi tepat antara keduanya dalam perbanyakan MA (Glomus sp). Penelitian dilaksanakan pada bulan Februari hingga Juli 2021 di Rumah Kaca Politeknik Pembangunan Pertanian Malang. Pengambilan sampel tanah dilakukan di Kelurahan Margomulyo Kecamatan Samboja, Kutai Kartanegara, Kalimantan Timur. Analisis tanah dan tanaman dilakukan di Laboratorium Biologi dan Kimia Tanah Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya. Penelitian menggunakan Rancangan Acak Lengkap Faktorial (RALF) dengan faktor pertama adalah tanaman inang antara lain jagung, milet, sorgum dan faktor kedua adalah dosis vermikompos berbeda meliputi V0 (0 g pot-1) (kontrol), V1 (1,7 g pot -1), V2 (2 g pot-1), V3 (2,3 g pot-1). Media tanam menggunakan tanah pasca tambang batu bara sebanyak 2 kg pot-1. Parameter Pengamatan meliputi jumlah spora MA, kolonisasi MA pada akar, pH (H2O), P total, P tersedia, serapan P tajuk tanaman, tinggi tanaman dan bobot kering tajuk tanaman. Analisis data dilakukan secara statistik menggunakan software Genstat dan Microsoft Excel 2016 dengan uji F (5%) untuk mengetahui pengaruh dari setiap perlakuan, dilanjutkan dengan uji Duncan Multiple’s Range Test (DMRT) (5%), dan uji korelasi dan regresi. Hasil penelitian menunjukkan dosis vermikompos dan jenis tanaman inang berpengaruh nyata terhadap jumlah spora, kolonisasi MA pada akar, pH (H2O), P tersedia, serapan P tajuk, bobot kering tajuk, dan tinggi tanaman. Namun, pada parameter P total, hanya dosis vermikompos yang memberikan pengaruh yang nyata. Penambahan vermikompos mampu meningkatkan jumlah spora sebesar 20,64-34,58% dan kolonisasi pada akar sebesar 21,07-41,22% pada masing-masing tanaman inang. Kombinasi dosis vermikompos sebanyak 2 g pot-1 dan milet sebagai tanaman inang menghasilkan jumlah spora MA (Glomus sp) tertinggi sebanyak 244 spora 100 g tanah-1 (4.880 spora pot-1) dengan kolonisasi pada akar mencapai 63,3%. Kolonisasi MA pada akar, pH (H2O), dan P tersedia menunjukkan hubungan sangat erat dengan jumlah spora dalam media perbanyakan yang ditunjukkan oleh nilai r pada uji korelasi berturut-turut sebesar (r=0,92), (r=0,54), (r=0,66).
English Abstract
Glomus sp is an arbuscular mycorrhiza (AM) that can be found in post-coal mining land in Margomulyo Village, Samboja District, Kutai Kartanegara Regency, East Kalimantan Province as many as 100-196 spores of 100 g soil-1 with colonization reaching 81%. Glomus sp has the potential to be used to help accelerate land revegetation due to its ability to adapt to the original environmental conditions. This condition is in contrast to the number of AM which tends to be limited, easily damaged and lost so that AM spores (Glomus sp) are required to propagate through pot culture. Corn, millet, and sorghum can be used as hosts of choice for AM spore propagation, while vermicompost can be used as an alternative nutrient source for propagation media. The purpose of this study was to analyze the addition of vermicompost, effect of corn, millet, and sorghum as host plant, and the correct combination in AM (Glomus sp) propagation. The research was carried out from February to July 2021 at the Greenhouse of the Malang Agricultural Development Polytechnic. Soil sampling was carried out in Margomulyo Village, Samboja District, Kutai Kartanegara, East Kalimantan. Soil and plant analysis was carried out in the Lab. Biology and Chemistry, Department of Soil, Faculty of Agriculture, Brawijaya University. The study used a completely randomized factorial design (CRFD) with the first factor being host plants including corn, millet, sorghum and the second factor being different doses of vermicompost including V0 (0 g pot-1) (control), V1 (1,7 g pot-1), V2 (2 g pot-1), V3 (2,3 g pot-1). Planting media using post-coal mining soil as much as 2 kg pot-1. Observation parameters included the number of AM spores, AM colonization in roots, pH (H2O), total P, available P, plant shoot P uptake, plant height and shoot dry weight. Data analysis was carried out statistically using Genstat software and Microsoft Excel 2016 with the F test (5%), then continued with Duncan Multiple's Range Test (DMRT) (5%) until correlation and regression tests. The results showed that the dose of vermicompost and the type of host plant significantly affected to the number of spores, AM colonization on roots, pH (H2O), available P, shoot P uptake, shoot dry weight, and plant height. However, on the total P parameter, only the vermicompost dose had a significant effect. The addition of vermicompost can increase the number of spores 20,64-34,58% and the percentage of colonization on the roots 21,07-41,22% of each host plant The combination dose of vermicompost as much as 2 g pot-1 and millet as a host plant produced the highest number of AM spores (Glomus sp) as much as 244 spores 100 g soil-1 (4.880 spores pot-1) with root colonization reaching 63,3%. AM colonization on roots, pH (H2O), and available P showed a very close relationship with the number of spores in the propagation medium as indicated by the value of r in the correlation test, respectively (r=0,92), (r=0,54), (r=0,66).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522040374 |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture > 338.16 Production efficiency |
| Divisions: | Fakultas Pertanian > Agroekoteknologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 03 Apr 2023 03:26 |
| Last Modified: | 03 Apr 2023 03:26 |
| URI: | http://repository.ub.ac.id/id/eprint/197810 |
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