Pentacolis., Kevin Maris and Suprayogi, STP, MP, PhD and Dr. Ir. Nur Hidayat, MP (2020) Pengaruh Konsentrasi Substrat Crude Glycerol terhadap Produksi Polihidroksialkanoat (PHA) Menggunakan Mikroba Konsorsia. Sarjana thesis, Universitas Brawijaya.
Abstract
Saat ini banyak penelitian mengenai penggunaan PHA (salah satu bahan baku pembuatan bioplastik) sebagai pengganti dari produksi plastik konvensional. Namun, produksi PHA masih sangat kecil dan terbatas. Hal ini disebabkan karena tingginya biaya produksi PHA. Pemanfaatan crude glycerol dan lumpur aktif sebagai sumber substrat/ sumber karbon dan bakteri penghasil PHA dapat menjadi solusi dalam penurunan biaya produksi PHA. Tujuan penelitian adalah menghasilkan PHA menggunakan crude glycerol dari mikroba konsorsia dan mengkarakterisasi PHA yang dihasilkan dari mikroba konsorsia berdasarkan perlakuan terbaik. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial dengan 6 (enam) taraf perlakuan yaitu konsentrasi crude glycerol sebesar 10%, 20%, 30%, 40%, 50%, dan 60% (w/v) dengan waktu inkubasi 72 jam dan dilakukan 3 (tiga) kali ulangan. Data yang diperoleh dari hasil penelitian dianalisis menggunakan Analysis of Variaces (ANOVA) dan dilanjutkan uji Duncan. Hasil penelitian diketahui perlakuan konsentrasi crude glycerol berpengaruh nyata terhadap berat sel kering, berat PHA, rendemen PHA dan kadar gliserol termetabolisme. Berat sel kering PHA terbaik didapatkan pada perlakuan konsentrasi crude glycerol 20 % (20 gL-1) sebesar 2,867 gL-1, berat PHA sebesar 1,200 gL-1 dan rendemen PHA sebesar 42,03 %. Kadar gliserol termetabolisme tertinggi pada perlakuan konsentrasi 10 % (10 gL-1) dengan nilai 44,30 %. Selain itu, karakteristik gugus fungsi dengan FTIR didapatkan gugus C- O-C polimerik, C=O ester yang merupakan ciri khas polihidroksialkanoat (PHA). Juga terdapat gugus fungsi CH3 dan CH2 yang menandakan ciri khas Poli(3- hidroksibutirat) (PHB). Hasil kenampakan mikrostruktur dengan SEM terlihat tidak beraturan dan memiliki pori bergelombang. Komponen elemen PHA yang didapatkan dengan EDX antara lain karbon (69,143%), oksingen (27,245%), sodium (1,644%), silika (0,295%), fosfat (0,37%), klorin (1,197%), dan kalium (0,106%).
English Abstract
Currently there are many studies on the use of PHA (one of the raw materials for making bioplastics) as a substitute for conventional plastic production. However, PHA production is still very small and limited. This is due to the high cost of PHA production. Utilization of crude glycerol and activated sludge as a source of substrate / carbon source and PHA-producing bacteria can be a solution in reducing the cost of PHA production. The research objective is to produce PHA using crude glycerol from microbial consortia and characterize PHA produced from microbial consortia based on the best treatment. The experimental design in this study used a non-factorial Completely Randomized Design (RAL) with 6 (six) levels of treatment namely crude glycerol concentration of 10%, 20%, 30%, 40%, 50%, and 60% (w / v) with incubation time 72 hours and performed 3 (three) replications. Data obtained from the results of the study were analyzed using Analysis of Variaces (ANOVA) and followed by Duncan's test. The results showed that the treatment of crude glycerol concentration significantly affected dry cell weight, PHA weight, yield of PHA and glycerol levels were metabolized. The best PHA dry cell weight was obtained in the treatment of crude glycerol concentration of 20% (20 gL-1) of 2.867 gL-1, PHA weight of 1,200 gL-1 and yield of PHA of 42.03%. The highest levels of glycerol are metabolized at a concentration of 10% (10 gL-1) with a value of 44.30%. In addition, the characteristics of functional groups with FTIR obtained polymeric C-O-C groups, C = O esters which are characteristic of polyhydroxyalkanoate (PHA). There are also CH3 and CH2 functional groups that characterize Poly (3-hydroxybutyrate) (PHB). The results of the appearance of microstructure with SEM looks irregular and has wavy pores. Components of PHA elements obtained with EDX include carbon (69.143%), oxingen (27.245%), sodium (1.644%), silica (0.295%), phosphate (0.37%), chlorine (1.197%), and potassium (0.106%).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0520100103 |
| Uncontrolled Keywords: | Crude glycerol, Mikroba Konsorsia, Polihidroksialkanoat, SEM-EDX, Crude glycerol, Microbial Consortium, Polihidroksialkanoat |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture > 338.16 Production efficiency |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Industri Pertanian |
| Depositing User: | Zainul Mustofa |
| Date Deposited: | 08 Jul 2022 07:15 |
| Last Modified: | 08 Jul 2022 07:15 |
| URI: | http://repository.ub.ac.id/id/eprint/191773 |
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