Aristya, Yuvira Ivana and Irnia Nurika,, STP,MP,PhD and Sri S.,, STP, M.Env.Mgt, PhD (2022) Pengaruh Penambahan MnSO4 Terhadap Aktivitas Enzim Lignolitik Bakteri Lysinibacillus sphaericus pada Degradasi Lignoselulosa dan Peningkatan Gas Metana dari Limbah Tandan Kosong Kelapa Sawit. Sarjana thesis, Universitas Brawijaya.
Abstract
Limbah tandan kosong kelapa sawit (TKKS) merupakan limbah pertanian yang memiliki kandungan lignoselulosa yang cukup tinggi sehingga berpotensi untuk dikembangkan menjadi biogas. Namun, tingginya kadar lignin pada TKKS dapat menghambat pembentukan gas metana, sehingga perlu dilakukan pretreatment untuk memudahkan proses pembentukan gas. Pada penelitian ini, pretreatment biologis dengan bakteri pendegradasi lignin Lysinibacillus sphaericus digunakan pada limbah TKKS. Bakteri L. sphaericus mampu menghasilkan enzim LiP, MnP, dan lakase yang berperan dalam degradasi lignin. Namun, untuk meningkatkan aktivitas enzim lignolitik dibutuhkan induser seperti MnSO4. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan MnSO4 terhadap aktivitas enzim lignolitik bakteri L. sphaericus dalam proses delignifikasi lignoselulosa dan pengaruhnya terhadap produksi gas metana. MnSO4 dengan konsentrasi 0 mM; 0,1 mM; dan 0,25 mM ditambahkan pada substrat yang telah diinokulasi dengan bakteri L. sphaericus dan diinkubasi selama 5 hari. Uji Biochemical Methane Potential (BMP) dilakukan untuk mengetahui potensi gas metana pada produksi biogas dari limbah TKKS. Pengukuran gas dilakukan setiap hari selama 28 hari. Penelitian ini menggunakan 6 sampel dengan 3 kali perulangan pada setiap sampel, meliputi blank sample, - celluloce, TKKS untreated, serta sampel TKKS hasil pretreatment dengan bakteri L. sphaericus tanpa penambahan MnSO4, dengan penambahan 0,1 mM MnSO4, dan penambahan 0,25 mM MnSO4. Perlakuan TKKS dengan bakteri L. sphaericus menghasilkan nilai total soluble phenol (TSP), total gula reduksi (TGR), susut berat, persentase lignin removal, dan nilai SMP yang lebih tinggi dibandingkan dengan sampel TKKS untreated. Hasil tersebut menunjukkan bahwa bakteri L. sphaericus berpotensi untuk digunakan dalam proses degradasi lignoselulosa TKKS. Hasil analisis ANOVA pada uji pH, TSP, TGR, dan susut berat menunjukkan terdapat hasil yang berbeda nyata antara sampel kontrol dan sampel dengan perlakuan bakteri dengan nilai signifikansi <0,05. Penambahan 0,25 mM MnSO4 menghasilkan peningkatan aktivitas enzim tertinggi yaitu enzim lakase hingga 44,71%, MnP 57,95%, dan LiP 54,72%, serta menunjukkan hasil uji tertinggi dibandingkan dengan perlakuan lainnya, yaitu nilai TSP 0,132 mg/g, TGR 6,504 mg/g, susut berat 47,49%, persentase lignin removal 38,56%, dan SMP 0,296 m3/kgVS. Hal ini menunjukkan bahwa penambahan induser mampu meningkatkan kemampuan bakteri pendegradasi lignin L. sphaericus dalam mendegradasi lignoselulosa dan meningkatkan hasil produksi gas metana dari TKKS
English Abstract
Oil palm empty fruit bunches (OPEFB) waste is agricultural wastes that have high lignocellulosic content so has the potential to be developed into biogas. However, high levels of lignin in OPEFB can inhibit the formation of methane gas, so necessary pretreatment is to facilitate the gas formation process. In this study, biological pretreatment with lignin- degrading bacteria Lysinibacillus sphaericus was used in OPEFB waste. Bacteria L. sphaericus is able to produce LiP, MnP, and laccase enzymes that play a role in lignin degradation. However, to increase the activity of ligninolytic enzymes needed inducer such as MnSO4.The purpose of this study was to determine the effect of MnSO4 on the ligninolytic enzyme activity of L. sphaericus in the lignocellulose degradation, and its effects on the production of methane. MnSO4 with a concentration of 0 mM; 0.1 mM; and 0.25 mM was added to the substrate that had been inoculated with bacteria L. sphaericus and incubated for 5 days. The Biochemical Methane Potential (BMP) test was carried out to determine the potential of methane gas in biogas production from OPEFB waste where gas measurements were carried out every day for 28 days. This study used 6 samples with 3 repetitions in each sample, including blank samples, - celluloce, OPEFB untreated, and samples of OPEFB pretreatment with bacteria L. sphaericus without the addition of MnSO4, with the addition of 0,1 mM MnSO4, and the addition of 0,25 mM MnSO4. OPEFB treatment with bacteria L. sphaericus resulted in higher total soluble phenol (TSP), total reducing sugar (TRS), weight loss, percentage of lignin removal, and SMP values which were higher than the OPEFB untreated. These results indicate that the bacteria L. sphaericus has the potential to be used in the lignocellulosic degradation process of OPEFB. The results of the ANOVA analysis on the pH, TSP, TGR, and weight loss tests showed that there were significantly different results between the control sample and the sample with bacterial treatment with a significance value of <0,05. The addition of 0,25 mM MnSO4 resulted in the highest increase in enzyme activity, where laccase enzyme up to 44,71%, MnP 57,95%, and LiP 54,72%, and also showed the highest test results compared to other treatments, where the TSP value of 0,132 mg/ g, TGR 6,504 mg/g, weight losses 47,49%, the percentage of lignin removal 38,56% and SMP 0,296 m3/kgVS. This indicates that the addition of inducers can increase the ability of lignin- degrading bacteria L. sphaericus in degrading lignocellulosic and increase the yield of methane gas from OPEFB.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522100011 |
| Uncontrolled Keywords: | Biogas, Lysinibacillus sphaericus, MnSO4, Pretreatment, TKKS,Biogas, Lysinibacillus sphaericus, MnSO4, OPEFB, Pretreatment |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture > 338.16 Production efficiency |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Industri Pertanian |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 08 Feb 2022 06:42 |
| Last Modified: | 18 Feb 2022 08:02 |
| URI: | http://repository.ub.ac.id/id/eprint/189556 |
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