Balqis, Feby Indriyani and Prof. Dr. Ir. Yunianta,, DEA and Jhauharotul M,, STP., MP (2021) Uji Aktivitas Sitotoksisitas dan Apoptosis Ekstrak Fukoidan Fraksi Air dari Sargassum Cristaefolium pada Sel Kanker Payudara MCF-7. Sarjana thesis, Universitas Brawijaya.
Abstract
Senyawa fukoidan merupakan kelompok polisakarida bersulfat yang terdapat pada alga coklat. Senyawa ini memiliki struktur dan komposisi kimia yang sangat beragam tergantung pada jenis dan kondisi lingkungannya. Diketahui fukoidan memiliki aktivitas biologis seperti antioksidan, antivirus dan antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak fukoidan dari alga coklat Sargassum cristaefolium fraksi air terhadap sitotoksisitas dan apoptosis sel kanker payudara MCF-7. Aktivitas sitotoksisitas dilakukan dengan uji MTT selama 24 jam menggunakan variasi ekstrak fukoidan sebesar 12,5; 25; 50; 100; 200; 400 dan 800 µg/ml. Selanjutnya viabilitas sel hidup dihitung menggunakan Haemocytometer dengan variasi waktu inkubasi sebesar 24, 48 dan 72 jam. Uji apoptosis dilakukan dengan pewarnaan sel menggunakan pewarna Hoechst 33342 dengan variasi 24, 48 dan 72 jam. Hasil penelitian menunjukkan adanya aktivitas sitotoksisitas sel MCF-7 dengan nilai IC50 sebesar 319,134 µg/ml. Hasil pada uji apoptosis menunjukkan bahwa ekstrak fukoidan dari Sargassum cristaefolium fraksi air mampu menginduksi apoptosis sel MCF-7. Hal tersebut menunjukan bahwa waktu inkubasi memiliki pengaruh signifikan pada viabilitas sel MCF-7. Pada perlakuan 24, 48 dan 72 jam dihasilkan persen viabilitas sel hidup sebesar 50,27%, 34,56 %, dan 29,97 %.
English Abstract
Fucoidan compound are groups of sulfated polysaccharides found in brown algae. These compounds have a very diverse structure and chemical composition depending on the type and environmental conditions. Fucoidan is known to have biological activities such as antioxidant, antiviral and antibacterial. This study aimed to determine the activity of fucoidan extract from brown algae Sargassum cristaefolium water fraction against cytotoxicity and apoptosis of MCF-7 breast cancer cells. Cytotoxicity activity was carried out by MTT test for 24 hours using a variation of fucoidan extract of 12.5; 25; 50; 100; 200; 400 and 800 µg/ml. Furthermore, the viability of living cells was calculated using a haemocytometer with variations in incubation time of 24, 48, and 72 hours. The apoptosis test was carried out by staining cells using the Hoechst 33342 with variations in 24, 48 and 72 hours. The results showed the presence of cytotoxicity activity of MCF-7 cells with an IC50 value of 319.134 µg/ml. The results of the apoptosis test showed that the aqueous fraction of fucoidan extract from Sargassum cristaefolium was able to induce apoptosis of MCF-7 cells. It was shown that incubation time had a significant effect on the viability of MCF-7 cells. In the 24, 48 and 72 hours treatment, the percentage of viability of live cells was 50.27%, 34.56 %, and 29.97%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0521100210 |
| Uncontrolled Keywords: | Senyawa fukoidan, Sargassum cristaefolium, sitotoksisitas, apoptosis, MCF-7,Fucoidan compounds, Sargassum cristaefolium, cytotoxicity, apoptosis, MCF-7 |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture > 338.16 Production efficiency |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 30 Dec 2021 01:30 |
| Last Modified: | 22 Feb 2022 07:14 |
| URI: | http://repository.ub.ac.id/id/eprint/187718 |
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Text (DALAM MASA EMBARGO)
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