Prayanti, Dwi Esti and Dr.Ir.Joni Kusnadi, M.Si and Baiq Dina Mariana, S.P.,M.Sc (2019) Analisis Kekerabatan Koleksi Lengkeng (Dimorcapus Longan L.) Balitjestro Berdasarkan Karakteristik Pembungaan Dengan Marka Issr (Inter Simple Sequence Repeats). Sarjana thesis, Universitas Brawijaya.
Abstract
Pembungaan lengkeng pada umumnya dipacu suhu 15-22oC. Lengkeng dapat diklasifikasikan berdasarkan respon pembungaannya terhadap suhu, yaitu mudah berbunga (sepanjang tahun dan ketika kebutuhan suhu tercapai) dan sulit berbunga (perlu induksi pembungaan). Penelitian ini bertujuan untuk menganalisa kekerabatan 12 aksesi lengkeng berdasarkan tiga karakter pembungaan dengan 12 marka ISSR (Inter Simple Sequence Repeats). Skoring data dilakukan berdasarkan hasil amplifikasi dan dianalisa menggunakan program NTSYS. Hasil penelitian menunjukkan bahwa tingkat kemiripan 12 aksesi lengkeng berkisar 65-94% dan terbagi dalam tiga kelompok. Kelompok pertama memiliki tingkat kemiripan 69.8-94%, terdiri dari 5 aksesi yang mewakili karakter sulit berbunga (perlu induksi pembungaan). Kelompok kedua memiliki tingkat kemiripan 71.8-82.2%, terdiri dari 5 aksesi yang mewakili karakter mudah berbunga (ketika kebutuhan suhu tercapai). Kelompok ketiga memiliki tingkat kemiripan 76.8%, terdiri dari 2 aksesi yang mewakili karakter mudah berbunga (sepanjang tahun). Aksesi yang memiliki kekerabatan terdekat adalah KL 04 dan KL 05 dengan tingkat kemiripan 94%. Marka ISSR yang digunakan berhasil mendeteksi kekerabatan lengkeng sesuai dengan karakter pembungaannya dengan baik.
English Abstract
Flowering of longan is generally driven by the temperature of 15-22oC. Longan can be classified based on the flowering responses to the temperature, which are easy to flower (throughout the year and when temperature requirement is reached) and difficult to flower (needs flowering induction). The aim of this research was to analyze the genetic relationship of 12 longan accessions based on three flowering characters by 12 ISSR (Inter Simple Sequence Repeats) markers. Data scoring was based on the results of amplification and analyzed using NTSYS program. The result showed that the similarity level of 12 longan accessions ranged 65-94% and divided into three groups. The similarity level of the first group was 69.8-94%, consisted of 5 accessions representing the character of difficult to flower (needs flowering induction). The similarity level of the second group was 71.8-82.2%, consisted of 5 accessions representing the character of easy to flower (when temperature requirement is reached). The similarity level of the third group was 76.8%, consisted of 2 accessions representing the character of easy to flower (throughout the year). Accessions having the closest genetic relationship were KL 04 and KL 05 with 94% of similarity level. In this research, ISSR markers were able to detect the genetic relationship of longan according to the flowering characters successfully.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2019/612/052002499 |
| Uncontrolled Keywords: | ISSR, kekerabatan, pembungaan lengkeng,Genetic relationship, ISSR, longan flowering |
| Subjects: | 300 Social sciences > 338 Production > 338.1 Agriculture > 338.16 Production efficiency |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 20 Dec 2021 03:44 |
| Last Modified: | 29 Mar 2022 01:39 |
| URI: | http://repository.ub.ac.id/id/eprint/187497 |
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