Kholifah Kf, Nur (2019) Penapisan Bakteri Penghasil Enzim Selulase Dari Feses Sapi (Bos Taurus). Sarjana thesis, Universitas Brawijaya.
Abstract
Selulase merupakan enzim ekstraseluler mendegradasi selulosa dan melepaskan gula reduksi sebagai produk akhirnya. Enzim selulase digunakan untuk penanganan air limbah, pengolahan kopi, memperhalus bubur kertas, menjaga warna kain, dan meningkatkan nutrisi pakan ternak. Kebutuhan enzim selulase yang banyak tidak diimbangi persediaan enzim sehingga indonesia memenuhinya dengan cara impor. Produksi komersial enzim biasanya menggunakan fungi atau bakteri. Data statistik provinsi Jatim menunjukkan jumlah sapi sebesar 3,953 ekor pada tahun 2017. Seekor sapi dapat mengahsilkan 8-10kg feses perhari. Feses sapi masih memiliki banyak kandungan mikroba karenanya, feses sapi merupakan sumber yang potensial untuk mengisolasi bakteri penghasil enzim selulase. Isolasi dilakukan dengan menginokulasikan feses sapi ke media CMC, koloni yang tumbuh diwarnai dengan Congo red dimana terdapat 4 koloni positif penghasil selulase ditandai dengan adanya zona bening. Zona bening digunaan untuk mengukur lindeks selulolitik dengan hasil S2-8 2,40 (IS), S2-9 1,07 (IS), S2-10 2,18 (IS), dan S2-31 1,53 (IS). Koloni bakteri diidentifikasi dengan pewarnaan Gram dan pengamatan morfologi bakteri. Koloni bakteri positif dikonfirmasi menggunakan uji DNS dimana hasil aktivitas enzim terbesar akan dilanjutkan dengan uji aktivitas enzim selulase untuk mengetahui waktu inkubasi optimum. Hasil konfirmasi bakteri S2-8 memiliki aktivitas enzim sebesar 0,018 U/mL dan hasil uji aktivitas enzim selulase sebesar 0,037 U/mL dengan waktu inkubasi optimum 4 hari.
English Abstract
Cellulase is an extracellular enzyme that degrades cellulose and releases reducing sugars as the final product. Cellulase enzymes are used for handling wastewater, processing coffee, refining pulp, maintaining the color of the fabric, and improving the nutrition of animal feed. Cellulase enzyme needs that are not much offset the supply of enzymes so that Indonesia meets them by import. Commercial production of enzymes usually uses fungi or bacteria. Statistical data on East Java province shows the number of cows was 3,953 in 2017. A cow can produce 8-10kg of feces per day. Cow dung still have a lot of microbial content; therefore, cow dung are a potential source for isolating the cellulase-producing bacteria. Isolation is done by inoculating cow dung into CMC media, the colonies are stained with Congo red where there are 4 positive colonies that produce cellulase by the presence of a clear zone. Clear zone is used to measure cellulolytic index with results of S2-8 2.40 (IS), S2-9 1.07 (IS), S2-10 2.18 (IS), and S2-31 1.53 (IS). Bacterial colonies were identified by Gram staining and observation of bacterial morphology. Positive bacterial colonies were confirmed using a DNS test where the results of the greatest enzyme activity would be followed by cellulase enzyme activity tests to determine the optimum incubation time. The results of confirmation of bacteria S2-8 have an enzyme activity of 0.018 U / mL and the results of the cellulase enzyme activity test of 0.037 U / mL with an optimum incubation time of 4 days.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2019/590/052002637 |
| Uncontrolled Keywords: | Bakteri, Selulase, Feses Sapi, Bacteria, Cellulase, Cow Dung |
| Subjects: | 500 Natural sciences and mathematics > 572 Biochemistry > 572.7 Enzymes > 572.756 Enzymes catalyzing reactions of specific chemicals (Cellulases) |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 30 Sep 2020 05:04 |
| Last Modified: | 24 Oct 2021 02:34 |
| URI: | http://repository.ub.ac.id/id/eprint/181655 |
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