Budiharjo, Stevani (2019) Pengaruh Kombinasi Monosodium Glutamat dan Diet Tinggi Lemakterhadap Kadar BDNF Otak Tikus Wistar Dewasa Betina. Sarjana thesis, Universitas Brawijaya.
Abstract
BDNF merupakan salah satu dari 4 neurotrophin yang termasuk kedalam golongan neurotropic factordan memiliki beberapa fungsi, seperti perkembangan sinaps, plastisitas sinaps, fungsi kognitif, proses inflamasi atau metabolik, danlong term potentiation (LTP). Diketahui bahwa kombinasi monosodium glutamat dan diet tinggilemakdapat menurunkan kadar BDNF otak yang berarti dapat mengganggu fungsi otak, terutama kognitif. Untuk mengetahui lebih lanjut maka dilakukan penelitian ini dengan menggunakan metode rancangan randomized control gorup post test designdan dilakukan pada tikus Wistar betina. Sampel dipilih secara acak dan dibagi dalam 6 kelompok, yaitu kontrol negatif (-), kontrol (+) 1 diet tinggi lemak (DTL), kontrol (+) 2 MSG 0,7mg/gBB, Perlakuan 1 (DTL+MSG 0,05mg/gBB), Perlakuan 2 (DTL+MSG 0,2mg/gBB), dan Perlakuan 3 (DTL+MSG 0,35mg/gBB). Masing-masing kelompok terdiri dari 5 ekor tikus dan dilakukan selama 56 hari, laluckadar BDNF diukur dengan menggunakan ELISA. Hasil penelitian menunjukkan peningkatkan kadar BDNF otak pada kelompok kontrol (+) 1 (p=0.585), kontrol (+) 2 ( p= 0.027), dan perlakuan 3 (p=0.027). Kesimpulan dari penelitian ini adalah pemberian MSG dosis tinggi dan kombinasi MSG dosis harian dengan diet tinggi lemak meningkatkan kadar BDNF otak, sedangkan diet tinggi lemak tidak memberikan efek yang signifikan terhadap kadar BDNF otak.
English Abstract
BDNF is one of the 4 neurotrophin that belongs to the group of neurotropic factors and has several functions, such as synaptic development, synapse plasticity, cognitive function, inflammatory or metabolic processes, and long term potentiation (LTP). It is known that the combination of monosodium glutamate and a high-fat diet can reduce brain BDNF levels which means it can interfere with brain function, especially cognitive. To find out more, this study was carried out using a randomized control design method of post-test gorup and performed on female Wistar rats. Samples were randomly selected and divided into 6 groups, namely negative control (-), control (+) 1 high fat diet (DTL), control (+) 2 MSG 0.7 mg / gBB, Treatment 1 (DTL + MSG 0.05 mg) / gBB), Treatment 2 (DTL + MSG 0.2 mg / gBB), and Treatment 3 (DTL + MSG 0.35 mg / gBB). Each group consisted of 5 mice and carried out for 56 days, then the BDNF was measured using ELISA. The results showed an increase in brain BDNF levels in the control group (+) 1 (p = 0.585), control (+) 2 (p = 0.027), and treatment 3 (p = 0.027). The conclusion of this study is the administration of high-dose MSG and combination of daily-dose MSG with a high-fat diet increases brain BDNF levels, whereas a high-fat diet does not have a significant effect on brain BDNF levels.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/412/052001257 |
| Uncontrolled Keywords: | BDNF, Monosodium Glutamat, Diet TinggiLemak-BDNF, Monosodium Glutamate, High Fat Dietary |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 19 Jul 2020 07:22 |
| Last Modified: | 22 Oct 2021 07:24 |
| URI: | http://repository.ub.ac.id/id/eprint/180021 |
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