Pramesti, Theresa Lidya (2019) Pengaruh Preventif Pemberian Susu Kuda Sumbawa Terhadap Ekspresi Il-6 Dan Histopatologi Lambung Pada Tikus Putih (Rattus Norvegicus) Model Ibd (Inflamatory Bowel Disease) Hasil Induksi Indometasin. Sarjana thesis, Universitas Brawijaya.
Abstract
Inflammatory Bowel Disease (IBD) merupakan penyakit yang ditandai dengan adanya inflamasi pada saluran pencernaan yang terkait dengan perubahan histopatologi mukosa pada saluran pencernaan seperti lambung, usus dan kolon dalam bentuk kerusakan epitel di lamina propria mukosa. Tikus putih hewan model IBD (Inflammatory Bowel Disease) dapat di buat menggunakan indometasin yang masuk dalam golongan Non Steroidal Antiinflamation Drug (NSAIDs). Indometasin dalam pembuatan hewan model IBD mengakibatkan perubahan histologi lambung dan memicu sitokin pro-inflamasi seperti IL-6 pada fase inflamasi. Penangananan saat ini banyak dikembangkan cara preventif sebagai alternatif untuk mencegah timbulnya suatu penyakit, salah satunya dengan susu kuda Sumbawa yang mengandung komponen bioaktif sebagai antioksidan eksogen, antiinflamasi dan antimikroba. Penelitian ini bersifat eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL). Tikus jantan dengan berat 150-200 gram dan berumur 8 minggu dibagi dalam lima kelompok perlakuan, yaitu kontrol negatif (tikus sehat), kontrol positif (tikus tanpa pemberian susu kuda Sumbawa, dan diinduksi indometasin dengan dosis 15mg/kgBB), perlakuan 1, 2, dan 3 yaitu tikus pemberian susu kuda Sumbawa dengan dosis 0,5mL, 1mL, 1,5mL dan diinduksi indometasin dengan dosis 15mg/kgBB. Parameter yang diamati adalah ekspresi IL-6 dan histopatologi lambung. Pengamatan IL-6 menggunakan metode Flowcytometri, sedangkan histopatologi lambung menggunakan pewarnaan Hematoksilin-Eosin (HE). Hasil penelitian ini menunjukan bahwa tikus yang diberi preventif susu kuda Sumbawa menunjukan adanya peningkatan ekspresi IL-6 sangat nyata (p<0.01) dan mampu memperbaiki kerusukan gambaran histopatologi organ lambung tikus model IBD. Kesimpulan penelitian yaitu pemberian susu kuda Sumbawa mampu mencegah kenaikan ekspresi IL-6 dan mencegah terjadi erosi sel mukosa lambung akibat indometasin.
English Abstract
Inflammatory bowel disease (IBD) is characterized by the presence of inflammation in the digestive tract. IBD is associated to the changes in the histopathology of the mucous of the digestive tract such as the stomach, the intestines and the colon in the form of damage in the lamina propria mucous. White rat animal models of IBD can be made by using indomethacin which belongs to the Non Steroidal Anti-Inflammatory Drug (NSAIDs) group. In the process of making the IBD model animals, indomethacin is being used to create some changes in histology of the stomach and trigger the pro-inflammatory cytokine such as IL-6 in inflammatory phase. Many treatments have been improved preventively as an alternative to prevent diseases to occur, one of which is Sumbawa horse milk which contains bioactive components as exogenous antioxidants, anti-inflammatory and antimicrobial. This research is experimental using a completely randomized design (CRD). Male rats weighing 150-200 grams and 8 weeks old were divided into five treatment groups, namely negative controls (healthy rat), positive controls (rat without Sumbawa horse milk, and indomethacin induced at a dose of 15 mg / kg), treatment 1, 2 and 3, namely rats given Sumbawa horse milk at a dose of 0.5mL, 1mL, 1.5mL and indomethacin induced at a dose of 15mg / kgBB. The parameters observed were expression of IL-6 and gastric histopathology. Observation of IL-6 using Flowcytometry method, while gastric histopathology uses Hematoxylin-Eosin (HE) staining. The results of this study showed that rats given preventive Sumbawa horse milk showed a very significant increase in IL-6 expression (p<0.01) and were able to repair histopathological damage of IBD gastric gastric organ models. The conclusion of the study was that Sumbawa horse milk was able to prevent the increase of IL-6 expression and prevent the erosion of gastric mucosa due to indomethacin.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/135/051909065 |
| Uncontrolled Keywords: | IBD (Inflammatory Bowel Disease), Indometasin, preventif, susu kuda Sumbawa, IL-6, dan Histopatologi Lambung, IBD (Inflammatory Bowel Disease), Indomethacin, preventive, Sumbawa Horse milk, IL-6, and Gastric Histopathology. |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.085 Feeds and applied nutrition > 636.085 2 Applied nutrition |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 06:41 |
| Last Modified: | 21 Oct 2021 04:32 |
| URI: | http://repository.ub.ac.id/id/eprint/176978 |
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