Nurhuda, Ahmad (2019) Pengaruh Pemberian Bekatul Terhadap Kadar Kolesterol Total, Hdl, Dan Gambaran Histopatologi Jantung Pada Tikus (Rattus Norvegicus) Dengan Diet Tinggi Kolesterol. Sarjana thesis, Universitas Brawijaya.
Abstract
Pemberian pakan tinggi kolesterol pada pet animal, dapat menyebabkan gangguan metabolisme berupa hiperkolesterolemia. Peningkatan kadar kolesterol mengakibatkan produksi asam empedu dan radikal bebas meningkat. Kolesterol merupakan senyawa steroid yang terdapat di dalam tubuh hewan dan manusia. Kadar kolesterol total normal pada tikus yaitu 10-54 mg/dL dan kadar HDL plasma darah tikus yang normal yaitu 35-85 mg/dL. Bekatul mempunyai kandungan serat kasar dan antioksidan yang dapat digunakan untuk mengatasi keadaan tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh dari bekatul sebagai terapi pada tikus (Rattus norvegicus) model diet tinggi kolesterol terhadap kadar kolesterol total, HDL dan gambaran histopatologi jantung. Tikus (Rattus norvegicus) dibagi dalam 5 kelompok dengan 4 kali pengulangan. Kelompok A sebagai kontrol negatif, kelompok B kontrol diberi pakan tinggi kolesterol, kelompok C tikus model diet tinggi kolesterol dengan terapi bekatul 3,2 g/ekor/hari, kelompok D tikus model diet tinggi kolesterol dengan terapi bekatul 7,6 g/ekor/hari, dan kelompok E tikus model diet tinggi kolesterol dengan terapi bekatul 11,4 g/ekor/hari. Terapi bekatul dilakukan selama 21 hari. Kadar kolesterol total dan HDL dihitung dengan metode spektofotometer dan gambaran histopatologi jantung diamati dengan pewarnaan HE (Hematoksillin-Eosin). Pengaruh pemberian bekatul terhadap kadar kolesterol total dan HDL dalam darah dianalisis dengan one way ANOVA (Analysis of Variance) dengan tingkat kepercayaan α=5% dan analisis histopatologi jantung secara kualitatif deskriptif. Hasil penelitian menunjukkan bahwa pemberian bekatul dapat menurunkan kadar kolesterol total dan meningkatkan kadar HDL secara signifikan (p<0,05), dengan dosis terbaik 11,4 g/ekor/hari dalam menurunkan kadar kolesterol total dan menaikkan kadar HDL. Terapi bekatul juga terbukti memperbaiki gambaran histopatologi jantung tikus dengan diet tinggi kolesterol.
English Abstract
The diet that contains high cholesterol given to pet animal cause metabolic disorder such as hypercholesterolemia. The increasing of cholesterol level causes thr production of bile acids and free radicals increases too. Cholesterol is a steroid compound found in animals and humans. Normal rats have total cholesterol level 10-54 mg/dL and HDL level 35-85 mg/dL in blood. Bran has a crude fiber content and antioxidants that can be used to resolve these conditions. This study aims to determine the effect of bran as therapy in rat (Rattus norvegicus) high-cholesterol dietary model towards total cholesterol levels, HDL, and histopathologic of heart. Rat (Rattus norvegicus) is divided into 5 groups with 4 repetitions. Group A as negative control, group B was fed high cholesterol, group C rat high-cholesterol diet model with bran therapy of 3,2 g/rat/day, group D rat high-cholesterol diet model with bran therapy of 7,6 g/rat/day, and group E rat diet high cholesterol model with bran therapy of 11,4 g/rat/day. Bran therapy performed for 21 days. Total cholesterol and HDL levels were determined by the spectofotometer method and heart histopathologic features were observed with HE staining (Hematoxillin-Eosin). The pathology of rice bran on blood total cholesterol and HDL levels was analyzed by one way ANOVA (Analysis of Variance) with α=5% and heart histopathologic analysis was qualitatively descriptive. The results showed that the administration of rice bran could reduce total cholesterol and increase HDL significantly (p<0.05) with the best dose of 11,4 g/rat/day. The rice bran therapy was also shown to improve the histopathology of rats with high cholesterol diets
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/37/051908975 |
| Uncontrolled Keywords: | Kolesterol total, HDL, Bekatul, Rattus norvegicus, dan Jantung, Total cholesterol, HDL, Rice bran, Rattus norvegicus, and Heart |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 05:54 |
| Last Modified: | 21 Oct 2021 03:03 |
| URI: | http://repository.ub.ac.id/id/eprint/176760 |
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