Nuruzzaman, Moch Filosofi (2019) Pengaruh Pemberian Diet Tinggi Lemak Terhadap Kadar Mda Pankreas Dan Histopatologi Pankreas Tikus Putih (Rattus Novergicus) Yang Diberi Paparan Immobilization Stress. Sarjana thesis, Universitas Brawijaya.
Abstract
Perubahan sosial dan transisi gizi dikalangan masyarakat berdampak pada pola hidup yang kurang baik. Keadaan yang ditimbulkan dari konsumsi makan yang kurang tepat dan stress berdampak pada organ pankreas. Pankreas mengalami terganggunya fungsi sebagai kelenjar eksokrin,menghasilkan enzim lipase menguraikan lemak menjadi asam lemak. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian diet tinggi lemak terhadap kadar MDA dan histopatologi pankreas tikus putih (Rattus Norvegicus) yang dipapar immobilization stress. Penelitian ini menggunakan tikus putih berusia 3-4 bulan, berat badan 150-250 gram sebanyak 20 ekor. Tikus dibagi menjadi 4 kelompok dengan kelompok 1 kontrol negatif, kelompok 2 perlakuan stress selama 3 jam stress, 2 jam istirahat dan di perlakuakan stress kembali 3 jam, kelompok 3 perlakuan diet tinggi lemak diberikan dengan metode sonde lambung sebanyak 5mL/ekor/hari yang dibagi tiga kali pemberian yaitu 2mL pagi, 1mL siang dan 2mL sore, kelompok 4 perlakuan kombinasi (stress dan diet tinggi lemak) lalu tikus di eutanasi pada hari ke 5. Analisa data dilakukan secara kuantitatif yaitu pemeriksaan kadar MDA, dianalisa menggunakan uji ANOVA taraf kepercayaan 95% (α=0.05) dan dilanjutkan uji Tukey (α=0.05) aplikasi statistik SPSS 21.0, sedangkan data kualitatif yaitu gambaran histopatologi pankreas pada masing masing kelompok perlakuan yang di sajikan secara deskriptif. Hasil penelitian pemberian diet tinggi lemak pada tikus putih yang di papar immobilization stress (kombinasi) mampu meningkatkan kadar MDA yang sangat signifikan (P<0,01) hingga mencapai rata-rata 581,50±30,00ng/mL di banding dengan kontrol negatif sebesar 440,50±12,32ng/mL, perlakuan stress sebesar 480,50±24,01ng/mL dan diet tinggi lemak sebesar 528,00±26,76ng/mL, sedangkan pemberian diet tinggi lemak yang di papar immobilization stress (kombinasi) juga dapat memperparah kerusakan histopatologi jaringan pankreas yang terlihat adanya perlemakan, hemoragi, infiltrasi sel radang, kongesti dan nekrosis. Kesimpulan dari penelitian ini pemberian diet tinggi lemak dan perlakuan immobilization stress (kombinasi) dapat meningkatkan kadar MDA dan peningkatan kerusakan histopatologi pankreas
English Abstract
Social change and nutrition transition among the community have an impact on poor lifestyle. Consuming unhealthy food and stress can take affect to pancreatic organs. The pancreas has a malfunction as an exocrine gland, producing lipase enzymes to break down fat into fatty acids. The aimed of this study to determine the effect of giving a highfat diet to MDA levels and pancreas histopathology of rat (Rattus norvegicus) exposed by immobilization stress. This studyused white rats aged 3-4 months with 150-250 grams of body weight. There are 20 rats were divided into 4 groups with group 1 : negative control, group 2 : stress treatment performed for 3 hours of stress, 2 hours of rest and then stressed 3 hours, group 3 : treatment with high fat diet that was given with 5ml/rat/day divided into three treatment, namely 2mL morning, 1mL afternoon and 2mL afternoon, group 4 : combination treatment (stress and high fat diet) and rat euthanized on day 5. Quantitative data used measurement of pancreatic MDA levels analyzed by ANOVA and continued to Tukey test, while qualitative data were histopathological description of pancreas in treatment group analyzed and presented descriptively.The results of the study of giving high-fat diets to rats exposed by immobilization stress (combination) were able to increase MDA levels highly significant (P<0.01) to reach an average of 581.50 ± 30.00 ng/mL compared to negative controls in the amount of 440.50 ± 12.32 ng/mL, the stress treatment about 480.50 ± 24.01 ng/mL and a high-fat diet was 528.00 ± 26.76 ng/mL, while giving a high-fat diet in rat exposed by immobilization stress (combination) can also aggravate the histopathological damage of pancreatic tissue that seen by fatty, hemorrhagic, inflammatory cell infiltration, congestion and necrosis.The conclusion of this study for given of high-fat diets and immobilization stress treatment (combination) ware able to increase MDA levels and relevate histopathological damage to the pancreas.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/39/051908977 |
| Uncontrolled Keywords: | Stress, Diet tinggi lemak, Kadar MDA, Histopatologi Pankreas, Tikus putih (Rattus Norvegicus), Stress, Hight fat, MDA, Rattus norvegicus, Histopathology pancreatic |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics |
| Divisions: | Profesi Kedokteran > Spesialis Dokter Hewan, Fakultas Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 05:43 |
| Last Modified: | 21 Oct 2021 03:04 |
| URI: | http://repository.ub.ac.id/id/eprint/176645 |
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