Purwanti, Hesti (2017) Gambaran Jumlah Peak Dan Berat Molekul Protein Spesifik Urin Pada Nefritis Lupus Dan Hubungannya Dengan Indeks Kronisitas Penyakit. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang: Nefritis Lupus (NL) merupakan manifestasi serius pada Lupus Eritematosus Sistemik (LES) yang merupakan prediktor utama untuk prognosis buruk LES. Perubahan patologis pada membran basalis dan selektivitas protein serta gangguan fungsi reabsorbsi tubulus proksimalis menyebabkan adanya pola protein urin yang diekskresikan pada NL. Pola protein spesifik urin ini diharapkan dapat digunakan untuk menilai derajat kronisitas penyakit NL Tujuan: Mengetahui gambaran jumlah peak dan berat molekul protein spesifik urin pada NL dan hubungannya dengan indeks kronisitas. Metode: Penelitian ini merupakan studi deskriptif dan cross sectional pada 20 orang pasien NL dengan pemeriksaan kromatografi kolom dan SDS-PAGE untuk menilai protein spesifik urin. Biopsi ginjal dilakukan untuk menilai kelas histopatologi dan indeks kronisitas. Korelasi antara jumlah peak dan berat molekul protein spesifik urin dilakukan dengan uji Pearson. Hasil : Gambaran protein spesifik urin pada NL indeks kronisitas tinggi mempunyai jumlah lebih banyak dan jenis bervariasi. Hubungan jumlah peak dengan indeks kronisitas tidak bermakna (p=0,132), sedangkan hubungan berat molekul bermakna (p=0,001). Kesimpulan: Gambaran protein spesifik urin pada NL mempunyai jumlah dan jenis yang bervariasi. Hubungan yang bermakna ditemukan antara berat molekul protein dengan indeks kronisitas penyakit, secara klinis menunjukkan semakin banyak protein BM rendah seiring dengan peningkatan kerusakan ginjal. Sedangkan jumlah peak dengan indeks kronisitas menunjukkan hubungan yang idak bermakna.
English Abstract
Background: Lupus Nephritis (LN) is the most serious manifestation in Systemic Lupus Erythematosus (SLE) that can be main predictor for poor prognosis in SLE. Current biomarkers for evaluating activity and chronicity LN are considered to be unsatisfactory ad renal biopsy is still to be “gold sandart”. Histopathology class and chronicity index are considered to evaluate disease progressivity and prognostic prediction. Pathological changes in glomerular basement membrane and selectivity of electrical charge are causing specify patterns of urine proteins excretion. The identification of this urine spesfic proteins are expected to help for disease progressivity evaluation and prognostic factor LN.. Objective: To identify urine specific proteins pattern in histology classes and chronicity index of LN. Methods: A descriptive and crosssection study in 20 NL patients. The patterns of urine specific proteins were determined using column chromatography and SDS-PAGE test to measure protein molecular weight. Renal biopsy to measure histopathology classes of LN and chronicity index. Correlation between urine specific proteins and index chronicity index with Pearson test. Result: High index chronicity NL patients shows more urine specific proteins with more types. There was no signicant correlation between peak protein and chronicity index with (p=0,132; r=0,349), and there were strong negative correlation between molecular weight protein and chronicity index (p=0,001; r=0,842). Conclusion: There was significant negative correlation between molecular weight protein and chronicity index, but there was no significant correlation between peak protein and chronicity index in NL patients.
Other obstract
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| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/572.6/PUR/g/2017/041802322 |
| Uncontrolled Keywords: | PROTEINS - DISEASEA, PROTEINS - BIOCHEMISTY |
| Subjects: | 500 Natural sciences and mathematics > 572 Biochemistry > 572.6 Proteins |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Penyakit Dalam, Fakultas Kedokteran |
| Depositing User: | Endang Susworini |
| Date Deposited: | 17 Nov 2019 04:09 |
| Last Modified: | 17 Nov 2019 04:09 |
| URI: | http://repository.ub.ac.id/id/eprint/175600 |
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