Dhara, Maria Yasintha Vega (2018) Pengaruh Pemberian Ekstrak Bikotans Terhadap Gambaran Mikroskopis Organ Hati Dari Mencit (Mus Musculus) Yang Terpapar Radiasi Gamma. Sarjana thesis, Universitas Brawijaya.
Abstract
Radiasi gamma sering digunakan baik untuk radiografi dan radioterapi. Sinar gamma memiliki energi yang besar dibandingkan dengan radiasi elektromagnetik lain sehingga dapat menembus jaringan manusia cukup jauh dan membunuh sel kanker. Namun di sisi lain, penyerapan energi radiasi ke dalam tubuh biologis dapat menyebabkan radikal bebas pada organ hati. Tujuan dari penelitian ini adalah mengetahui pengaruh dari radiasi gamma terhadap gambaran mikroskopis organ hati mencit dan pengaruh pemberian ekstrak beluntas, kenikir, mahkota dewa, daun katuk dan bunga sepatu (Bikotans). Penelitian ini dilakukan dengan cara mencit dipapari radiasi gamma tanpa pemberian ekstrak dengan 5 variasi waktu untuk mendapatkan dosis paparan maksimumnya. Kemudian, mencit diberi ekstrak dengan variasi dosis 3,18 mg, 4,18 mg, 5,18 mg, 6,18 mg dan 7,18 mg dengan durasi paparan 40 menit. Mencit kemudian dibedah dan dibuat preparat organ hati. Kerusakan organ dapat dilihat dari gambaran mikroskopis dengan perbesaran 400x. Hasil penelitian menunujukkan sebelum diberi ekstrak Bikotans, kerusakan total sel hepatosit adalah 54,43%. Setelah diberi ekstrak Bikotans kerusakan total sel hepatosit adalah 18,14%. Dosis ekstrak 3,18 mg merupakan dosis efektif, karena pada dosis tersebut persentase kerusakan sel berkurang paling besar yaitu 28,81%. . Kata kunci: Radiasi Gamma, Ekstrak Bikotans, Organ Hati, Mencit (Mus musculus), Gambaran Mikroskopis. vi (Halaman ini sengaja dikosongkan) vii THE EFFECT OF GIVING BIKOTANS EXTRACT TO MICROSCOPIC IMAGE OF MICE (Mus musculus) LIVER EXPOSED BY GAMMA RADIATION ABSTRACT Gamma radiation is frequently used for radiographic and radiotherapy. The gamma rays have greater energi than any other kind of electromagnetic radiations, that it can penetrate human tissue deep enough. On the other hand, the radiation energi that absorbed by the body may generate free radicals. The purpose of this study is to analyze the effects of gamma radiation on microscopic image of mice’s liver and also the effects of indian fleabane, kenikir, god’s crown, star gooseberry, and hibiscus (Bikotans) extract. This research was conducted by exposing mice to gamma radiation without giving extract with 5 variant time dose to get maximum doses of gamma radiation. Afterward, the mice were given extract with 5 variant dose: 3,18 mg, 4,18 mg, 5,18 mg, 6,18 mg and 7,18 mg, under 40 minutes of gamma radiation exposure. The mice then dissected and the liver preparations were made. The structural damaged of liver cells were observed through microscope with 400x magnification. The result showed that before being given Bikotans extract, the total of hepatocyte cell damage was 54.43%. After being given Bikotans extract, the total damage of hepatocyte cells was 18.14%. The most effective dose was 3,18 mg, because after being given 3,18 mg of extract the percentage of cell damage decreases considerably i.e 28,81%.
English Abstract
Gamma radiation is frequently used for radiographic and radiotherapy. The gamma rays have greater energi than any other kind of electromagnetic radiations, that it can penetrate human tissue deep enough. On the other hand, the radiation energi that absorbed by the body may generate free radicals. The purpose of this study is to analyze the effects of gamma radiation on microscopic image of mice’s liver and also the effects of indian fleabane, kenikir, god’s crown, star gooseberry, and hibiscus (Bikotans) extract. This research was conducted by exposing mice to gamma radiation without giving extract with 5 variant time dose to get maximum doses of gamma radiation. Afterward, the mice were given extract with 5 variant dose: 3,18 mg, 4,18 mg, 5,18 mg, 6,18 mg and 7,18 mg, under 40 minutes of gamma radiation exposure. The mice then dissected and the liver preparations were made. The structural damaged of liver cells were observed through microscope with 400x magnification. The result showed that before being given Bikotans extract, the total of hepatocyte cell damage was 54.43%. After being given Bikotans extract, the total damage of hepatocyte cells was 18.14%. The most effective dose was 3,18 mg, because after being given 3,18 mg of extract the percentage of cell damage decreases considerably i.e 28,81%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/225/051806163 |
| Uncontrolled Keywords: | Radiasi Gamma, Ekstrak Bikotans, Organ Hati, Mencit (Mus musculus), Gambaran Mikroskopis. Microscopic Image, Bikotans Extract, Liver, Gamma radiation, Mice (Mus musculus) |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.321 Drugs derived from plants |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Fisika |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 04 Jun 2020 03:37 |
| Last Modified: | 18 Oct 2021 03:37 |
| URI: | http://repository.ub.ac.id/id/eprint/168150 |
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