Destianie, Elissa (2018) Pengaruh Pemberian Kombinasi Monosodium Glutamat Dan Diet Tinggi Lemak Terhadap Ketebalan Endometrium Tikus Putih (Rattus Novergicus. Sarjana thesis, Universitas Brawijaya.
Abstract
Faktor nutrisi dapat mempengaruhi kesehatan reproduksi terutama fertilitas. Penelitian mengenai konsumsi MSG dan diet tinggi lemak yang berlebih terbukti dapat berpengaruh negatif pada organ reproduksi dan menyebabkan infertilitas. Penelitian lain membuktikan bahwa pemberian MSG dengan dosis rendah tidak berpengaruh negatif secara nyata terhadap organ reproduksi. Konsumsi MSG bersamaan dengan diet tinggi lemak terbukti meningkatkan kadar lemak total, tetapi belum terbukti memberikan efek negatif pada sistem reproduksi terutama endometrium. Maka dari itu, tujuan dari penelitian ini adalah melihat pengaruh kombinasi MSG dengan dosis yang disesuaikan dengan rata-rata konsumsi harian manusia dan diet tinggi lemak terhadap ketebalan endometrium. Penelitian ini dilakukan pada tikus putih Rattus novergicus betina dengan usia 6-8 minggu dan berat 140-200 gram. Penelitian dilakukan selama 56 hari terdiri dari 6 kelompok yaitu kontrol negatif, K(+1) DTL, K(+2) MSG 0,7mg/gram BB, P1 DTL+MSG 0,05mg/gram BB, P2 DTL+MSG 0,20mg/gram BB, P3 DTL+MSG 0,35 mg/gram BB. Pembedahan tikus dilakukan pada hari ke 57-61 saat fase proestrus. Hasil uji one way ANOVA, rerata ketebalan endometrium (p=0,045) pada kelompok yang diberikan kombinasi MSG dan DTL menurun secara signifikan dibandingkan dengan kontrol negatif. Penurunan yang signifikan juga terjadi pada perbandingan ketebalan endometrium kelompok kombinasi MSG dan DTL terhadap kelompok kontrol positif diet tinggi lemak (K+1) dan MSG 0,7mg/gram BB (K+2). Hasil penelitian ini membuktikan bahwa pemberian kombinasi MSG dan diet tinggi lemak berakibat pada penurunan ketebalan endometrium.
English Abstract
Nutritional factors can affect reproductive health, include fertility. Study about the consumption of MSG and excessive high-fat diet can negatively affect the reproductive organs and cause infertility has been proven. Other studies declared that the administration of MSG with low doses not affect the organs negatively. Consumption of MSG and high-fat diet combination increase total fat, but study about the direct effect of administration high-fat diet and MSG to reproduction system especially endometrium has not been proven. Therefore, the aim of the present study was to investigate the effect of high-fat diet and MSG with a dose adjusted by the average daily consumption of humans on endometrial thickness. This study was conducted on female white rat (Rattus novergicus) age 6-8 weeks and weight 140-200 gram. This 56-day study divided into six groups: 1) negative control, 2) K (+1): HFD, 3) K(+2): MSG 0,7 mg/gram BB, 4) P1: HFD+MSG 0,05 mg/gram BB, 5) P2: HFD+MSG 0,20 mg/gram BB, and 6) P3: HFD+MSG 0,35 mg/gram BB. Rats dissection were conducted on day 56-61 during proestrus phase. Results of a one-way ANOVA showed that there were significant reduction of endometrial thickness between combination of MSG and HFD administration group with negatif control group (p=0,045). Significant result on endometrial thickness also occurred in comparison of group combination of MSG and HFD to the positive control group (K+1) high-fat diet administration and (K+2) MSG 0,7 mg/gram BB administration(p=0,015).The results of this study proved that administration of a combination of MSG and high-fat diet affect endometrial thickness degression.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/210/051805451 |
| Uncontrolled Keywords: | MSG, diet tinggi lemak, ketebalan endometrium, MSG, high fat diet, endometrium thickness |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics |
| Divisions: | Fakultas Kedokteran > Kebidanan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 26 Apr 2020 06:19 |
| Last Modified: | 20 Oct 2021 06:30 |
| URI: | http://repository.ub.ac.id/id/eprint/167469 |
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