Wibowo, Geofanny Tri (2018) Produksi Enzim Selulase Dari Fungi Trichoderma Harzianum Dengan Memanfaatkan Rumput Gajah (Pennisetum Purpureum) Sebagai Substrat (Kajian Konsentrasi Inokulum Dan Waktu Inkubasi. Sarjana thesis, Universitas Brawijaya.
Abstract
Rumput gajah (Pennisetum purpureum) merupakan tanaman jenis rumput-rumputan yang memiliki produktivitas tinggi namun kurang dimanfaatkan dengan baik. Rumput gajah diketahui banyak mengandung selulosa sehingga berpotensi sebagai bahan baku pengganti pati dalam menghasilkan glukosa. Dalam pemanfaatannya, selulosa harus dihidrolisis terlebih dahulu dengan menggunakan enzim selulase. Enzim selulase merupakan enzim kompleks yang memotong secara bertahap rantai selulosa menjadi glukosa. Selulase tersebut dapat diperoleh dari hewan ruminansia maupun mikroorganisme. Trichoderma harzianum dikenal sebagai salah satu mikroorganisme yang dapat menghasilkan enzim selulase. Trichoderma harzianum memiliki status GRAS (Generally Regarded As Safe) yang telah dievaluasi oleh FDA (U.S Food and Drug Administration). Penelitian ini bertujuan untuk mengetahui pengaruh massa substrat dan waktu inkubasi terhadap aktivitas enzim selulase yang dihasilkan oleh Trichoderma harzianum sehingga diperoleh kondisi optimum produksi enzim selulase. Pada penelitian ini menggunakan metode Rancangan Acak Kelompok Faktorial (RAKF) dengan 2 faktor. Faktor I yaitu konsentrasi inokulum terhadap massa substrat (11%, 14%, dan 20% (v/b)). Faktor II yaitu waktu inkubasi (144 jam, 168 jam, dan 192 jam) sehingga didapatkan 9 kombinasi perlakuan. Masing-masing perlakuan diulang sebanyak 3 kali, sehingga didapatkan 27 satuan percobaan. Selanjutnya dilakukan uji aktivitas enzim secara kualitatif menggunakan larutan congo red untuk melihat viii terbentuknya zona bening pada media CMC dan secara kuantitatif dengan metode spektrofotometri menggunakan reagen DNS. Data yang diperoleh dianalisa dengan Analysis of Variance (ANOVA) menggunakan General Linear Model menggunakan aplikasi Minitab 17 dan dilakukan uji lanjut dengan uji BNT (Beda Nyata Terkecil) apabila terdapat pengaruh faktor yang signifikan dengan selang kepercayaan 95%. Berdasarkan hasil penelitian yang didapatkan, terdapat pengaruh nyata (α = 0,05) waktu inkubasi terhadap aktivitas enzim selulase dan tidak terdapat pengaruh nyata (α = 0,05) konsentrasi inokulum terhadap aktivitas enzim selulase. Aktivitas enzim selulase tertinggi diperoleh dengan perlakuan waktu inkubasi 168 jam dan konsentrasi inokulum 11% berdasarkan pengujian aktivitas enzim selulase secara kuantitatif dengan nilai aktivitas sebesar 0.7142±0.0627 IU/ml.
English Abstract
Elephant grass (Pennisetum purpureum) is a type of grass that has high productivity but not well utilized. Elephant grass is known to contain a lot of cellulose so its potential as the substitute for starch in producing glucose. In its use, cellulose must be hydrolyzed first by using cellulase enzymes. Cellulase enzyme is a complex enzyme that cuts the chain of cellulose into glucose gradually. The cellulase can be obtained from ruminant animals and microorganisms. Trichoderma harzianum is known as one of the microorganisms that can produce cellulase enzymes. Trichoderma harzianum has a GRAS (Generally Regarded As Safe) status that has been evaluated by the FDA (U.S Food and Drug Administration). This study aims to determine the effect of substrate mass and incubation time on the activity of cellulase enzymes produced by Trichoderma harzianum in order to obtain optimum conditions for cellulase enzyme production. The method used in this research was Factorial Randomized Block Design with 2 factors. The first factor is the concentration of inoculum (11%, 14%, and 20% (v/w)). The second factor is the incubation time (144 hours, 168 hours and 192 hours) so that there are 9 treatment combinations. Each treatment was repeated 3 times, so that 27 experimental units were obtained. Furthermore, qualitative enzyme activity test was performed using congo red solution to see the formation of clear zones on CMC media and quantitatively by spectrophotometric method using DNS reagents. The data obtained were analyzed with Analysis of Variance (ANOVA) using the General Linear Model using the Minitab 17 application and further testing was carried out with the BNT test (the Smallest Real Difference) if x there was a significant factor influence with 95% confidence interval. Based on the results, there was a significant effect (α = 0.05) of the incubation time factor on cellulase enzyme activity and there was no significant effect (α = 0.05) inoculum concentration factor on cellulase enzyme activity. The highest cellulase enzyme activity was obtained with 168 hours of incubation time and 11% inoculum concentration based on quantitative cellulase enzyme activity testing with an activity value of 0.7142 ± 0.0627 IU / ml.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2018/511/051810601 |
| Uncontrolled Keywords: | Aktivitas Enzim Selulase, Enzim Selulase, Rumput Gajah, Trichoderma harzianum,/Cellulase Enzyme, Cellulase Enzyme Activity, Elephant Grass, Trichoderma harzianum |
| Subjects: | 500 Natural sciences and mathematics > 572 Biochemistry > 572.7 Enzymes > 572.756 Enzymes catalyzing reactions of specific chemicals (Cellulases) |
| Divisions: | Fakultas Teknologi Pertanian > Keteknikan Pertanian |
| Depositing User: | Endang Susworini |
| Date Deposited: | 23 Oct 2019 02:18 |
| Last Modified: | 17 Mar 2022 07:00 |
| URI: | http://repository.ub.ac.id/id/eprint/165198 |
![[thumbnail of Geofanny Tri Wibowo.pdf]](http://repository.ub.ac.id/style/images/fileicons/text.png) |
Text
Geofanny Tri Wibowo.pdf Restricted to Registered users only Download (2MB) |
Actions (login required)
 |
View Item |