Dari, Mimin Wulan (2018) Pengaruh Pemberian Bekatul Terhadap Kadar SGOT dan SGPT serta Gambaran Histopatologi Hepar pada Tikus Putih (Rattus norvegicus) Model Diet Tinggi Kolesterol. Sarjana thesis, Universitas Brawijaya.
Abstract
Hiperkolesterolemia merupakan suatu gangguan metabolisme yang ditandai dengan peningkatan kadar kolesterol total dalam darah yang melebihi nilai normal. Kadar kolesterol total normal pada tikus yaitu 10-54 mg/dL. Tingginya kadar kolesterol dalam darah, maka tubuh akan menyeimbangkannya dengan mensintesis asam empedu. Sintesis asam empedu dan produksi radikal bebas yang berlebihan akan menyebabkan stres oksidatif. Stres oksidatif akan meningkatkan terjadinya peroksidasi lipid sehingga menyebabkan kerusakan membran sel hepar yang akan meningkatkan kadar SGOT dan SGPT dalam darah serta perubahan gambaran histologi hepar. Kandungan serat kasar dan antioksidan dalam bekatul mampu mengatasi hal tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh bekatul sebagai terapi pada tikus putih (Rattus norvegicus) model diet tinggi kolesterol terhadap kadar SGOT dan SGPT serta gambaran histopatologi hepar. Tikus putih (Rattus norvegicus) dibagi dalam 5 kelompok, yaitu: kelompok kontrol negatif, kelompok kontrol positif, kelompok terapi bekatul dengan dosis 16%/ekor/hari, 38%/ekor/hari dan 57%/ekor/hari. Terapi bekatul dilakukan selama 21 hari. Kadar SGOT dan SGPT dibaca dengan spektrofotometer dan gambaran histopatologi hepar diamati dengan pewarnaan HE (Hematoksillin-Eosin). Kadar SGOT dan SGPT dianalisis dengan one way ANOVA (Analysis of Variance) dengan tingkat kepercayaan α=5% dan histopatologi hepar dianalisis secara kualitatif deskriptif. Hasil penelitian menunjukkan pemberian bekatul dengan dosis 57%/ekor/hari secara signifikan (P<0,05) dapat menurunkan kadar SGOT dan SGPT. Pengamatan histopatologi menunjukkan bahwa antoksidan dalam bekatul dapat menurunkan perlemakan pada gambaran histopatologi hepar. Dosis bekatul 57%/ekor/hari menunjukkan dosis efektif dalam menurunkan kadar SGOT, SGPT dan dapat mengurangi perlemakan sel hepar. Kesimpulan dari penelitian ini bahwa pemberian bekatul dapat menurunkan kadar SGOT, SGPT dan memperbaiki gambaran histopatologi hepar tikus model diet tinggi kolesterol
English Abstract
Hypercholesterolaemia is a metabolic disorder on increase of total cholesterol levels in blood exceeding normal values. Total cholesterol normal levels in rat is 10-54 mg/dL. High cholesterol levels in the blood, then the body will balance it by synthesizing bile acids. The synthesis of bile acids and the production of excessive free radicals will cause oxidative stress. Oxidative stress will increase the occurrence of lipid peroxidation causing damage to hepatic cell membranes that will increase levels of SGOT and SGPT in the blood and changes in the histology of the liver. The content of crude fiber and antioxidants in bran are able to overcome the hypercholesterolemia. This study aimed to determine the effect of rice bran as a therapy in white rats (Rattus norvegicus) high-cholesterol diet model on levels of SGOT and SGPT and histopathologic images of hepar. Rats (Rattus norvegicus) were divided into 5 groups, namely: negative control group, positive control group, rice bran therapy group with dose 16% /rat/day, 38% /rat/day and 57%/ rat/day. Rice bran therapy performed for 21 days. SGOT and SGPT levels were determined by spectrophotometers and hepatic histopathologic images were observed with HE (Hematoxylin-Eosin) staining. The levels of SGOT and SGPT were analyzed by one way ANOVA (Analysis of Variance) with a belief level α = 5% and histopathology of liver was analyzed qualitatively descriptively. The results showed that rice bran with dose of 57% / rat / day significantly (P <0,05) can decrease SGOT and SGPT levels. Histopathologic observations showed that antioxidants in rice bran could reduce fatty acids on hepatic histopathology. The dosage of 57% / rat / day showed to decrease SGOT, SGPT levels and can reduce fatty acids on hepatic. It can be concluded that the administration of rice bran can reduce the levels of SGOT, SGPT and improve the histopathologic of liver rats high cholesterol dietary model
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/159/051810188 |
| Uncontrolled Keywords: | Bekatul, Hepar, Kolesterol, Rattus norvegicus, SGOT dan SGPT-Cholesterol, Liver, Rattus norvegicus, Rice bran, SGOT and SGPT. |
| Subjects: | 500 Natural sciences and mathematics > 572 Biochemistry > 572.5 Miscellaneous chemicals > 572.57 Lipids |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Sugeng Moelyono |
| Date Deposited: | 19 Mar 2019 08:38 |
| Last Modified: | 22 Oct 2021 03:45 |
| URI: | http://repository.ub.ac.id/id/eprint/161521 |
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