Citrawati, Putri (2018) Efek Pemberian Bekatul terhadap Kadar Trigliserida dan Gambaran Histopatologi Aorta pada Tikus (Rattus norvegicus) Model Diet Tinggi Kolesterol. Sarjana thesis, Universitas Brawijaya.
Abstract
Kolesterol merupakan senyawa steroid yang terdapat di dalam tubuh hewan dan manusia. Kadar kolesterol total normal pada tikus yaitu 10-54 mg/dL dan trigliserida 26-145 mg/dL. Kadar kolesterol dalam darah dapat mengalami peningkatan. Penyebab meningkatnya kadar kolesterol adalah makanan yang mengandung kolesterol tinggi. Akibatnya produksi asam empedu dan radikal bebas meningkat, sehingga kadar trigliserida akan meningkat dan menyebabkan kerusakan sel. Bekatul mempunyai kandungan serat kasar dan antioksidan yang dapat digunakan untuk mengatasi keadaan tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh dari bekatul sebagai terapi pada tikus (Rattus norvegicus) model diet tinggi kolesterol terhadap kadar trigliserida dan gambaran histopatologi aorta. Tikus (Rattus norvegicus) dibagi dalam 5 kelompok dengan 4 kali pengulangan. Kelompok A sebagai kontrol negatif, kelompok B kontrol diberi pakan tinggi kolesterol, kelompok C tikus model diet tinggi kolesterol dengan terapi bekatul 16%/ekor/hari, kelompok D tikus model diet tinggi kolesterol dengan terapi bekatul 38%/ekor/hari, dan kelompok E tikus model diet tinggi kolesterol dengan terapi bekatul 57%/ekor/hari. Terapi bekatul dilakukan selama 21 hari. Kadar trigliserida dihitung dengan metode GPO-PAP dan gambaran histopatologi aorta diamati dengan pewarnaan HE (HematoksillinEosin). Pengaruh pemberian bekatul terhadap kadar trigliserida dalam darah dianalisis dengan one way ANOVA (Analysis of Variance) dengan tingkat kepercayaan α=5% dan analisis histopatologi aorta secara kualitatif deskriptif. Hasil penelitian menunjukkan bahwa terapi bekatul dapat menurunkan kadar trigliserida secara sangat nyata (p<0,01) dengan dosis terapi 57%/ekor/hari menunjukkan dosis terbaik dalam menurunkan kadar trigliserida. Terapi bekatul juga terbukti dapat memperbaiki gambaran histopatologi aorta tikus model diet tinggi kolesterol
English Abstract
Cholesterol is a steroid compound found in animals and humans. Total cholesterol normal levels in rat is 10-54 mg/dL and triglyceride 26-145 mg/dL. Blood cholesterol levels may increase. The cause of increased levels of cholesterol is foods that contain high cholesterol. As a result the production of bile acids and free radicals increases, so triglyceride levels will increase and cause cell damage. Rice bran has a crude fiber content and antioxidants that can be used to resolve these conditions. This study aims to determine the effect of rice bran as therapy in rat (Rattus norvegicus) high-cholesterol dietary model towards triglyceride levels and histopathologic of aorta. Rat (Rattus norvegicus) is divided into 5 groups with 4 repetitions. Group A as negative control, group B was fed high cholesterol, group C rat high-cholesterol diet model with rice bran therapy of 16%/rat/day, group D rat high-cholesterol diet model with rice bran therapy of 38%/rat/day, and group E rat diet high cholesterol model with rice bran therapy of 57%/rat/day. Rice bran therapy performed for 21 days. Triglyceride levels were calculated by the GPO-PAP method and aortic histopathologic features were observed with HE staining (Hematoxillin-Eosin).The effect of rice braning on blood triglyceride levels was analyzed by one way ANOVA (Analysis of Variance) with α=5% and aortic histopathologic analysis was qualitatively descriptive. The results showed that rice bran therapy could significantly reduce triglyceride levels (p <0,01) with a dose of 57%/rat/day to decrease triglyceride levels. Rice bran therapy is also repair aortic tissue histopathology of rat high cholesterol diet model
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/107/051808778 |
| Uncontrolled Keywords: | Kolesterol, Trigliserida, Bekatul, Rattus norvegicus, dan Aorta- Cholesterol, Triglyceride, Rice Bran, Rattus norvegicus, and Aorta |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics > 613.28 Specific nutritive elements |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 18 Mar 2019 02:06 |
| Last Modified: | 21 Oct 2021 22:49 |
| URI: | http://repository.ub.ac.id/id/eprint/161470 |
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