Nurmayani, Imami (2016) Uji Efek Antifungi Ekstrak Ethanol Kulit Buah Delima Merah (Punica Granatum Linn.) Terhadap Pertumbuhan Candida Albicans Secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Jamur Candida albicans dikenal sebagai penyebab terbanyak infeksi pada vagina. Sekitar 75% wanita pernah mengalami satu kali gejala infeksi vulvovaginal kandidiasis dan diantaranya mengalami infeksi berulang. Obat antifungi menimbulkan efek samping yang tidak nyaman. Kulit buah delima merah (Punica granatum Linn.) kaya akan kandungan senyawa kimia antara lain, polifenol, flavonoid, dan tannin yang diduga sebagai zat antifungi yang efektif. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak ethanol kulit buah delima merah memiliki efek antifungi terhadap pertumbuhan Candida albicans secara in vitro. Sampel diperoleh dari isolat jamur di Laboratorium Mikrobiologi FKUB. Konsentrasi ekstrak yang dipakai yaitu 0%, 7,5%, 10%, 12,5%, 15%, 17,5%, 20%, dan 22,5%. Metode yang digunakan adalah metode dilusi tabung. Hasil statistik one way ANOVA menunjukkan terdapat perbedaan yang signifikan pada perubahan konsentrasi ekstrak ethanol kulit buah delima merah terhadap jumlah koloni jamur Candida albicans (p<0,05). Uji korelasi menunjukkan adanya hubungan yang erat antara konsentrasi ekstrak dengan jumlah koloni (Korelasi, r = -0,879: p<0,05). Berdasarkan penelitian ini, dapat disimpulkan bahwa ekstrak ethanol kulit buah delima merah mempunyai efek antifungi terhadap Candida albicans dengan Kadar Bunuh Minimal (KBM) adalah 22,5%.
English Abstract
Candida albicans fungus are known as the most often cause of vaginal infection. About 75% woman have once experienced vulvovaginal candidiasis symptom and among them are reccurent. Antifungal medicine group induce uncomfortable side effects. Red pomegranate rind (Punica granatum Linn.) is rich of chemical compound such as polifenol, flavonoid, and tannin which have been presume as effective antifungal agents. The aim of this research is to know the effect of red pomegranate rind ethanolic extract against Candida albicans growth in vitro. This research uses tube dillusion methode. The sample is obtained from fungus isolate from Microbiology Laboratory of Medical Faculty of Brawijaya University. The extract concentration used are 0%, 7,5%, 10%, 12,5%, 15%, 17,5%, 20%, and 22,5%. The result of one-way-ANOVA statistics shows there is a significant difference in change of red pomegranate rind ethanolic extract concentration to the amount of Candida albicans colony (p<0,05). The correlation test shows there is a strong relation between the extract concentration and the colony amount (Correlation, r = -0,879: p<0,05). Based on this research, it can be concluded that the red pomegranate rind ethanolic extract has an antifungal effect against Candida albicans, with minimum fungicidal concentration of 22,5%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2016/499/ 051607395 |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics |
| Divisions: | Fakultas Kedokteran > Ilmu Gizi |
| Depositing User: | Kustati |
| Date Deposited: | 24 Aug 2016 11:15 |
| Last Modified: | 24 Aug 2016 11:15 |
| URI: | http://repository.ub.ac.id/id/eprint/126198 |
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