Mahmudah, Anis (2015) Analisis Antropometri pada Tikus Rattus norvegicus Galur Wistar Jantan yang Diberi Diet Normal Standar PAR-S dan Diet Normal Standar AIN-93M. Sarjana thesis, Universitas Brawijaya.
Abstract
Penelitian secara in vivo (menggunakan hewan coba) harus memperhatikan prinsip refinement. Refinement yaitu membebaskan hewan coba dari rasa haus dan lapar dengan pemberian diet yang terstandar. Pemilihan diet standar dalam penelitian sangatlah penting untuk menghindari hasil yang bias. Diet normal standar yang biasa digunakan dalam penelitian adalah diet normal standar PAR-S dan diet normal standar AIN-93M. Kedua jenis diet ini memiliki komposisi dan kandungan zat gizi yang berbeda. Pemberian diet standar pada hewan coba akan sangat berpengaruh terhadap antropometri (berat badan, panjang badan, lingkar toraks, dan lingkar perut). Penelitian ini bertujuan untuk mengetahui perbedaan hasil pengukuran antropometri pada tikus Rattus norvegicus galur wistar jantan yang diberikan diet normal standar PAR-S dan diet normal standar AIN-93M. Jenis penelitian ini adalah studi eksperimental menggunakan randomized control group post test design. Subjek penelitian adalah tikus Rattus norvegicus Galur Wistar jantan yang dipelihara di laboratorium Biosains Universitas Brawijaya, Malang selama 12 minggu. Pemilihan sampel dilakukan menggunakan metode RAL (Rancangan Acak Lengkap) dengan jumlah sampel total sebanyak 32 ekor tikus. Uji statistik berat badan dengan Mann-Whitney menunjukkan nilai p<0,001. Uji statistik panjang badan, lingkar toraks, dan lingkar perut dengan Independent t-test menunjukkan nilai p<0,001. Dari penelitian ini dapat disimpulkan bahwa terdapat perbedaan yang signifikan pada antropometri (berat badan, panjang badan, lingkar toraks dan lingkar perut) tikus yang diberi diet normal standar PAR-S dan diet normal standar AIN-93M
English Abstract
Research on in vivo (using animal models) must have been research principles of refinement. Refinement is avoid animals from thirst and hunger. Diet normal standard in study were diet normal standard PAR-S and normal standard AIN-93M. Both types of diet have been composition and nutrients content that different. Diet can be affect to anthropometry (weight, length,thoracic circumference and abdominal circumference) of animal models, so it takes a diet standard diet to avoid biased result. This research aimed to know the differences of measurement result of anthropometry on male Rattus norvegicus strain wistar that given by the diet normal standard PAR-S and normal standard AIN-93M. This type of research was experimental studies using randomized post test control group design. The subject was rat male Rattus norvegicus strain wistar was observed in the Bioscience laboratory of the University of Brawijaya Malang for 12 weeks.The selection of samples was carried out using Complete Random Design with 32 rat of total samples. Mann-Whitney test in weight rat shown p<0,001. Independent t-test in length, thoracic circumference and abdominal circumference rat shown p<0,001. The conclusion was differences between the anthropometry (weight, length,thoracic circumference and abdominal circumference) rat that given with diet normal standard PAR-S and normal standard diet AIN-93M
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2015/326/051502429 |
| Subjects: | 600 Technology (Applied sciences) > 613 Personal health and safety > 613.2 Dietetics |
| Depositing User: | Samsul Arifin |
| Date Deposited: | 23 Mar 2015 15:23 |
| Last Modified: | 19 Oct 2021 01:00 |
| URI: | http://repository.ub.ac.id/id/eprint/125087 |
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