Purnomo, AthayaFebriantyo (2015) Pengaruh Ekstrak Kulit Manggis (Garcinia mangostana) terhadap Daya Tahan Hidup, Laju Penetasan, dan Kecacatan pada Embrio Ikan Zebra (Danio rerio). Sarjana thesis, Universitas Brawijaya.
Abstract
Garcinia mangostana (manggis) memiliki berbagai manfaat, oleh karena berbagai bahan aktif di dalamnya. Garcinia mangostana dipercaya sebagai raja antioksidan di Indonesia, namun sebagaimana yang diketahui bahwa obat memiliki ambang batas dan efek toksik. Ketika terlalu banyak mengkonsumsinya, bisa menciptakan gangguan dalam metabolisme tubuh antara lain hemolisis, peningkatan limfosit, penurunan massa liver dan ginjal. Danio rerio (Ikan zebra) memiliki 70% kesamaan terhadap manusia dan digunakan sebagai hewan coba untuk uji toksisitas. Penelitian ini bertujuan untuk mengamati daya tahan hidup, laju penetasan, dan kecacatan pada embrio ikan zebra yang diberi ekstrak kulit manggis. Studi eksperimental menggunakan embrio ikan zebra ditempatkan di 6-Well plate, setiap Well diisi dengan 30 embrio dengan tiga macam konsentrasi ekstrak kulit manggis 1250, 1000, dan 750 µg/mL yang diamati tiap 24 jam selama 3 hari. Penelitian dilakukan triplikasi. Hasil penelitian menunjukkan bahwa pada konsentrasi 1250 µg /mL (ekstrak kulit manggis), daya tahan hidup embrio ikan zebra pada triplikasi 0% pada 24 hpf (hour post fertilization). Pada konsentrasi 1000 µg/mL, didapatkan rerata tingkat daya tahan hidup embrio 31,33+5,13%. Pada konsentrasi 750 µg/mL didapatkan rerata tingkat daya tahan hidup sebesar 77,33+10%. Laju penetasan hanya dapat diamati pada kelompok kontrol. Konsentrasi lethal ekstrak kulit manggis dicapai pada 716,651 µg/mL dengan dasar penentuan konsentrasi menggunakan analisis Probit. Kecacatan ditemukan mulai konsentrasi 750 µg/mL pada pengamatan 72 hpf, kecacatan yang teramati berupa bentuk curve-shaped, perikardium robek, detak jantung tidak terdeteksi meskipun pigmentasi telah terbentuk pada sebagian kulit embrio, perikardium membesar, dan denyut jantung tidak terdeteksi karena rongga perikardium keruh
English Abstract
Garcinia mangostana (Mangosteen) has a wide range of benefits, perhaps most result from xanthone phytoceuticals/antioxidants. Garcinia mangostana perceived in Indonesia as the king of the antioxidant, however we already know that every potent medication has a limitation on use and toxic level. The toxic level perceived to be the effect of other phytoceutical compound, named mangostin, that responsible for the mortality and morbidity mechanism of the animal model. Reported effect of the overconsuming mangosteen pericarp extract such as metabolic disorders, hemolysis, lymphocytosis, and decrease of liver and kidney masses. The purpose of this study is to know the acute toxicity level in terms of mortality rate, lethal concentration, and the teratogenic effect of mangosteen pericarp ethanolic extract toward zebrafish embryos. Experimental study used zebrafish embryos placed in 6-Well, each well contained 30 embryos divided in 4 groups, there were three groups were given standardized mangosteen pericarp ethanolic extract (1250, 1000, 750 µg/mL) and one control group was given with physiological embryonic medium which observed every 24 hours for 3 days and the study repeated three times. Zebrafish (Danio rerio) has 70% autologous toward human, therefore zebrafish also being used for toxicity test toward natural compound. The extract was given on 2 hpf (hour post-fertilization). The results show that on 1250 µg/mL concentration of mangosteen pericarp ethanolic extract, survival rate reaches 0% at three times repetition of the study since 24 hpf. At 1000 µg/mL concentration, the average of survival rates reach 31,33+5,13%. At 750 µg/mL concentration, the average of survival rates reach 77,33+10%. We were using SPSS Ver.22 program for Probit Analysis, the lethal concentration for half population (LC50) of mangosteen pericarp ethanolic extract was reached at 716,651 µg/mL. Heart rate can observed only in control group. Lethal concentration 50 (LC50) reach at 716,651 µg/mL. Defects were found at 750 µg/mL concentration at 72 hpf observation, in the forms of curved-shape body, broken or enlarged pericardium, and undetected heart rate because the pericardium cavity was murky although pigment has been formed in several skin parts of embryos.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2015/162/051501138 |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 13 Feb 2015 09:47 |
| Last Modified: | 22 Oct 2021 06:05 |
| URI: | http://repository.ub.ac.id/id/eprint/124906 |
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