Priadi, Gema Akbar (2018) Isolasi Dan Karakterisasi Bakteri Penyebab Penyakit Grain Rot Yang Disebabkan Oleh Burkholderia Glumae Pada Tanaman Padi Di Malang Raya. Sarjana thesis, Universitas Brawijaya.
Abstract
Penyakit busuk biji (grain rot) adalah salah satu penyakit pada tanaman padi yang disebabkan oleh bakteri Burkholderia glumae. Gejala pada pelepah, busuk berbentuk panjang vertikal, luka berwarna keabu-abuan dikelilingi oleh tepi berwarna coklat kemerahan gelap. Gabah menjadi berwarna lebih gelap dan memiliki garis coklat kemerahan malai di kuntum antara daerah berwarna terang dan malai berwarna lebih gelap. Di wilayah sentra produksi padi kabupaten Malang terdapat gejala bercak coklat pada pangkal dan abu-abu pada ujung gabah. Gejala tersebut memiliki kemiripan dengan gejala penyakit busuk biji yang disebabkan oleh bakteri B. glumae. Gejala becak coklat pada gabah padi ini diduga disebabkan oleh penyakit busuk biji. Penelitian bertujuan untuk mengkaji bakteri penyebab busuk biji pada tanaman padi dan sebaran penyakit busuk biji di Malang Raya serta mengkaji perbedaan karakteritik bakteri penyebab busuk biji dari beberapa daerah di Malang Raya Patogen bakteri di isolasi dari gabah atau biji yang berasal dari 17 kecamatan di Malang Raya. Penelitian dilaksanakan di Laboratorium Karantina Tumbuhan Balai Besar Karantina Pertanian (BBKP) Surabaya, penelitian terdiri dari tiga tahapan, yaitu : isolasi bakteri, karakterisasi bakteri, dan identifikasi molekuler. Ketiga tahapan tersebut terdiri dari kegiatan pengambilan sampel, isolasi bakteri di media selektif, uji hipersensitif dan patogenisitas, pengujian Gram, inokulasi bakteri pada kondisi anaerob, pengujian pigmen fluoresen, inokulasi bakteri pada media YDC, inokulasi bakteri pada D1M agar, pengujian oksidasi, pertumbuhan bakteri pada suhu 40°C, pertumbuhan bakteri pada pH yang berbeda, pertumbuhan pada kadar NaCl 3%, tes arginine dihidrolase, hidrolisis gelatin, hidrolisis pati, uji pemanfaatan sumber karbon, ekstraksi DNA, amplifikasi DNA, dan elektroforesis. Hasil isolasi dan karakterisasi menunjukan 7 isolat bakteri penyebab penyakit busuk biji memiliki karakterik yang positif pada uji patogenisitas dan hipersensitif. Semua bakteri termasuk kelompok Gram negatif dan bersifat aerob. Koloni tidak menghasilkan pigmen fluoresen pada media King’s B. Koloni tidak berwarna kuning pada media YDC. Tumbuh pada media D1M agar. Positif pada uji oksidase. Tumbuh pada pH 4 dan 8, pada suhu 40°C, dan pada kadar NaCl 3%. Negatif pada uji dihidrolisis arginine. Positif pada uji pencairan gelatin dan pada uji hidrolisis pati. Negatif pada pengujian media TSI. Positif pada deteksi PCR. Dari hasil penelitian ini dapat ditarik kesimpulan 7 bakteri penyebab busuk biji di Malang Raya adalah bakteri B. glumae.
English Abstract
The grain rot disease is one of the rice diseases that is caused by pathogenic bacterium Burkholderia glumae. The disease symptomps that are shown in rice sheath which are vertically rotten, and the wound that might be caused by this bacterium are gray and are surrounded by reddish-brown border. Panicle symptoms: straw-colored panicles containing florets with a darker base and a reddish-brown line across the floret between darker straw-colored and straw-colored areas. In the area of rice production centers of Malang Raya there are symptoms of brown spots on the base and gray at the end of grain. These symptoms bear a resemblance to the symptoms of grain rot disease caused by B. glumae bacteria. The phenomenon of brown spots on rice paddy is suspected to be caused by grain rot disease. This research aims to know the grain-rotcaused bacterium in Malang Raya and also to know the differences of grain rot caused bacterium characteristic in several places of Malang Raya. The bacteria pathogen were isolatd from grain or seed from 17 sub districts in Malang Raya. The research was conducted at Plant Quarantine Laboratory of Indonesian Center for Agricultural Quarantine (BBKP) Surabaya, the research were consisted of three stages, which were sampling, The study consists of three stages, which were: bacterial isolation, bacterial characterization, and molecular identification. The three stages consist of sampling, bacterial isolation in selective media, hypersensitive test and pathogenicity, Gram test, bacterial inoculation in anaerobic condition, fluorescent pigment test, bacterial inoculation on YDC media, bacterial inoculation in D1M agar, oxidase test, bacterial growth at 40 ° C, bacterial growth at different pH, growth at 3% NaCl, arginine dihydrolase test, gelatin hydrolysis, starch hydrolysis, carbon source utilization test, DNA extraction, DNA amplification, and electrophoresis. The results of isolation and characterization showed 7 isolats of bacteria that caused grain rot disease had a positive characteristic in pathogenicity and hypersensitivity test. All of bacteria were Gram-negative and also aerobic. The colonies did not produce fluorescent pigments on King's B. The colony did not show yellow colour in YDC media. The bacteria grew on D1M agar media and showed positive result on the oxidase test. It grew at pH 4 and 8, at 40°C, and at 3% NaCl. The bacteria showed negative result on arginine hydrolyzed test but showed positive in gelatin liquefaction test and on starch hydrolysis test. The another result had also showed that the bacteria showed negative result on TSI media test and positive result on PCR detection. Based on the results it could be concluded that 7 bacteria that caused grain rot disease in Malang Raya were B. glumae
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FP/2018/111/051802197 |
| Uncontrolled Keywords: | - |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.9 General topics of pest and disease control |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 22 May 2018 01:02 |
| Last Modified: | 20 Oct 2021 04:03 |
| URI: | http://repository.ub.ac.id/id/eprint/10776 |
Preview |
Text
BAB III.pdf Download (554kB) | Preview |
Preview |
Text
BAB IV.pdf Download (1MB) | Preview |
Preview |
Text
DAFTAR PUSTAKA.pdf Download (206kB) | Preview |
Preview |
Text
BAB V.pdf Download (115kB) | Preview |
Preview |
Text
BAGIAN DEPAN.pdf Download (1MB) | Preview |
Preview |
Text
LAMPIRAN.pdf Download (940kB) | Preview |
Preview |
Text
BAB I.pdf Download (591kB) | Preview |
Preview |
Text
BAB II.pdf Download (107kB) | Preview |
Actions (login required)
 |
View Item |