Ladamay, Nidha Arfa (2017) Potensi Senyawa Glukosamin Dan Kondroitin Sulfat Pada Produk Kaldu Dari Bubuk Ceker Ayam Sebagai Anti-Osteoarthritis Yang Diuji Secara Invivo. Magister thesis, Universitas Brawijaya.
Abstract
Osteoarthritis (OA) merupakan penyakit sendi degeneratif. Pengurangan perkembangan OA pada penderita dilakukan salah satunya dengan pemberian glukosamin (GS) dan kondroitin sulfat (CS). GSdan CS dari produk hewani selama ini mengandalkan sirip ikan hiu. Namun bahan baku sirip hiu sulit didapatkan, mahal dan terancam punah, sehingga diperlukan alternatif sumber tulang rawan yang memiliki kandungan nutrisi yang sama dengan sirip ikan hiu, misalnya ceker ayam. Ceker adalah kaki dari unggasayam yang mudah didapatkan, harganya murah dan tinggi komponen tulang rawan. Tulang rawan ayam memiliki kandungan CS2,17% dan kandungan GS12,98 % sehingga ceker ayam berpotensi sebagai anti-OA. Penelitian ini bertujuan untuk membandingkan metode rebus dan presto dalam pembuatan bubuk ceker ayam sebagai bahan baku kaldu ceker ayam terhadap kandungan GS dan CS tertinggi serta pengaruh kaldu ceker ayam, suplemen tulang rawan ceker ayam dan suplemen GS komersial terhadap sitokin TNF-α dan histopatologi tulang rawan sendi lutut tikus. Penelitian ini dilakukan secara bertahap, tahap pertama adalah optimasi bubuk ceker ayam sebagai bahan baku produk kaldu ceker ayam dengan metode rebus 5 menit dan presto 60 menit. Setiap perlakuan diulang sebanyak 5 kali sehingga didapatkan 10 unit perlakuan. Bubuk kaldu perlakuan terbaik dipilih menggunakan metode Zeleny. Tahap kedua adalah pengujian anti-OA secara in vivo dengan metode Rancangan Acak Lengkap (RAL) dengan perlakuan kontrol negatif (A1), kontrol positif (A2), produk bubuk kaldu ceker ayam terbaik (A3), bubuk suplemen ekstrak tulang rawan ceker ayam (A4), dan suplemen komersial (welmove) (A5) dengan melihat ekspresi TNF-α dan histopatologi tulang rawan sendi lutut tikus. Tikus diperlakukan OA dengan pemberian injeksi papain (4%) dan cysteine (0,03 M) melalui intra-articulary sendi lutut kanan pada hari ke1, ke4 dan ke7. Setiap perlakukan terdiri dari 5 ekor tikus. Analisa data menggunakan ANOVA dan uji lanjut BNT (α=5%). Hasil penelitian menunjukkan bahwa metode perebusan dan presto berpengaruh nyata terhadap kandungan CS (P<0,5 t-test), namun tidak berpengaruh nyata terhadap kandungan GS (P>0,5 t-test) produk bubuk kaldu ceker ayam. Perlakuan terbaik didapatkan dari produk bubuk kaldu ceker ayam rebus dengan kandungan GS 1,31±0,27% dan CS 0,81±0,02%. Pemberian bubuk kaldu ceker ayam, bubuk suplemen ekstrak tulang rawan ceker ayam dan suplemen tidak berpengaruh nyata (p>0,5) terhadap penurunan ekspresi TNF-α hewan uji tikus. Perlakuan bubuk suplemen ekstrak tulang rawan ceker ayam terbukti paling baik dalam mengurangi pembentukan OA dan didukung dengan hasil histopatologi dimana tulang rawan kelompok A4 memiliki lapisan superfisial yang teratur, morfologi sel normal dan populasi kondrosit normal.
English Abstract
Osteoarthritis (OA) constutes a degenerative joint disease that is accompanied by cartilage damage that causes pain and loss of motion. OA causes a decline in the quality of the work and daily life. Reduction in patients with OA development is done either by administration of glucosamine (GS) and chondroitin sulfate (CS). GS and CS of animal products have been relying on a shark's fin. However, shark fin raw materials are difficult to obtain, expensive or endangered. So it needs an alternative source of cartilage that contains the same nutrients as shark fin, such as chicken claw. Claw is a part of the chicken body that is easily available, cheap, and high component of cartilage. Chicken cartilage contains 2.17% CS and 12.98% GS so that the chicken claw has potential as an anti-OA. This study aimed to compare the boiling and presto methods in the manufacture of powdered chicken claw as a raw material in chicken claw broth on the highest content of GS and CS, influences of broth chicken claw, supplements chicken claw cartilage and supplements GS commercial on the expression of TNF-α and histopathology in rat knee joint cartilage. The research was carried out in several stages, the first stage was the optimization ofthe chicken claw powder as raw material for a chicken claw broth with the methodes boiling 5 minutes and presto 60 minutes. Each treatment was repeated 5 times to obtain 10 units of treatment. the best powdered brothwas selectedby using Zeleny method.The second stage was the testing of anti-OA in vivo method Completely Randomized Design (CRD) with negative control treatment (A1), a positive control treatment (A2), thebest broth powder productof chicken claw (A3), extract supplements of chicken claw cartilage powder (A4 ), and commercial supplements (welmove) (A5) to see the expression of TNF-α and histopathology in rat knee joint cartilage. The rats treated with the OA injection of papain (4%) and cysteine (0.03 M) through intra-articulary right knee joint on the day of 1st, 4th and 7th. Each treatment consisted of 5 rats. Data were analyzed using ANOVA and a further test BNT (α = 5%). The results showed that the boiling and presto methodes impacted on the content of CS (P <0.5 t-test) but did not significantly affected the content of the GS (P> 0.5 t-test) chicken claw broth powder products. The best treatment was obtained from boiling the chicken claw broth powder products with content of 1.31 ± 0.27% GS and 0.81 ± 0.02% CS. Giving chicken claw broth powder, powdered extract supplements of chicken claw cartilage and the supplements had no significant effect (p> 0.5) of the decrease in TNF-α expression rat animalstest. Treatment of powdered extract supplements of chicken claw cartilagewas proven the most successful in reducing the formation of OA and supported by histopathological results where Cartilage group A4 had a regular superficial layer, normal cell morphology and normal chondrocyte population.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.722 3/lad/p/2017/041710195 |
| Uncontrolled Keywords: | GLUCOSAMINE - THERAPEUTIC USE, OSTEOARTHRITIS, CHONDROITIN SULFATES, CHICKENS, CLAWS |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.7 Diseases of musculoskeletal system > 616.72 Diseases of joints > 616.722 Arthritis > 616.722 3 Osteoarthritis |
| Divisions: | S2/S3 > Magister Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 24 Apr 2018 06:10 |
| Last Modified: | 26 Nov 2021 06:53 |
| URI: | http://repository.ub.ac.id/id/eprint/9821 |
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