Malo, Margaretha Natalina (2017) Hubungan Antara Kadar Anti-Dsdna Dan Anti Sm Terhadap Beratnya Manifestasi Nefritis Lupus Di Rsu Dr. Saiful Anwar Malang. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang. Lupus Eritematosus Sistemik (LES) merupakan penyakit autoimun sistemik yang disebabkan produksi antibodi dan deposisi kompleks imun yang bermanifestasi sebagai kerusakan jaringan. Antibodi yang banyak ditemukan pada LES adalah anti dsDNA dan anti Sm. Anti dsDNA adalah antibodi terhadap antigen dsDNA. Anti Sm adalah antibodi terhadap antigen Smith. Keberadaan antibodi tersebut kemungkinan terkait dengan terjadinya NL. Tujuan Mengetahui apakah kadar anti ds-DNA dan anti Sm dalam serum berkaitan dengan derajat manifestasi lupus nefritis. Metode penelitian. Penelitian ini adalah crossectional study dengan pengambilan sampel dari bulan Maret 2009-Februari 2010. Subyek penelitian adalah 40 pasien LES, terbagi menjadi kelompok derajat (derajat 0-2 dan derajat 3-4) dan kelompok biopsi (kelas I-II, kelas III-IV, kelas V-VI). Pemeriksaan kadar Anti dsDNA dan Anti Sm menggunakan metode direct ELISA. Analisa data untuk kelompok derajat dengan uji Mann Whitney, dan untuk kelompok biopsi dengan uji Kruskal Wallis. Selanjutnya dilakukan uji korelasi Spearman. Hasil penelitian Rerata kadar anti dsDNA dan anti Sm kelompok derajat 3-4 lebih tinggi dibandingkan kelompok derajat 0-2 (p=0,000 dan p= 0.171). Rerata kadar Anti dsDNA paling tinggi pada kelompok biopsi kelas III-IV (p = 0,002), rerata kadar anti Sm paling tinggi pada kelompok biopsi kelas I-II (p=0,787). Terdapat korelasi positif antara anti dsDNA dengan kelompok derajat (r=0,611, p=0.000). Tidak terdapat korelasi yang bermakna antara Anti dsDNA dengan kelompok kelas biopsi, antara anti Sm dengan kelompok derajat, dan antara anti Sm dengan kelompok biopsi (p=0,370; p=0,168; dan p=0,611). Kesimpulan Kadar Anti dsDNA dan Anti Sm pada kelompok derajat 3-4 lebih tinggi daripada kelompok derajat 0-2. Kadar Anti dsDNA ditemukan paling tinggi pada kelompok biopsi kelas III-IV. Kadar Anti Sm ditemukan paling tinggi pada kelompok biopsi kelas I-II. Terdapat korelasi positif antara kadar Anti dsDNA dan kelompok derajat,
English Abstract
Background. Systemic Lupus Erythematosus (LES) is a systemic autoimmune disease caused by antibody production and immune complex deposition that manifests as tissue damage. Antibodies found in LES are anti dsDNA and anti Sm. Anti dsDNA is an antibody against dsDNA antigen. Anti Sm is an antibody against the Smith antigen. The presence of such antibodies may be related to the occurrence of NL. Objective. To determine whether the levels of anti-ds-DNA and anti-Sm in serum correlated with the degree of manifestation of lupus nephritis. Research methods. This study is a cross-sectional study with sampling from March 2009-February 2010. The subjects were 40 LES patients, divided into groups of derajat (derajat 0-2 and derajat 3-4) and biopsy group (class I-II, class III- IV, class V-VI). Anti dsDNA and Anti Sm examination using Direct ELISA method. Data analysis for group derajat with Mann Whitney test. Data analysis for biopsy group with Kruskal Wallis test. The Spearman correlation test is then performed. Results. Mean level of anti dsDNA group of derajat 3-4 higher than group of derajat 0-2 (p=0,000). The mean rate of Anti dsDNA of class III-IV biopsy group was higher than other biopsy group (p=0,002). Mean level of anti-Sm group of derajat 3-4 higher than group of derajat 0-2 (p=0,171). The mean rate of Anti Sm of class I-II biopsy group was higher than other biopsy group (p=0,787). There was a positive correlation between anti dsDNA and the derajat group (r=0.611, p=0.000). There was no significant correlation between Anti dsDNA and biopsy class group, between anti-Sm and derajat groups, and between anti-Sm and biopsy groups (p=0.370, p=0.168, and p=0.611). Conclusion. Anti dsDNA and Anti Sm levels in derajat group 3-4 were higher than the derajat group 0-2. Anti dsDNA levels were found to be highest in the class III-IV biopsy group. Anti Sm levels were found to be highest in the class I-II biopsy group. There was a positive correlation between Anti dsDNA levels and derajat groups, but there was no significant correlation between Anti dsDNA and biopsy class group, between anti-Sm and derajat groups, and between anti-Sm and biopsy class groups.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.772/MAL/h/2017/041709072 |
| Uncontrolled Keywords: | LUPUS NEPHRITS, ANTIBODY DIVERSITY, TUMOR ANTIGENS, ANTIGEN - ANTIBODY REACTIONS |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.7 Diseases of musculoskeletal system > 616.77 Diseases of connectives tissues > 616.772 Systemic lupus erythematosus |
| Divisions: | Profesi Kedokteran > Spesialis Patologi Klinik, Fakultas Kedokteran |
| Depositing User: | Nur Cholis |
| Date Deposited: | 13 Apr 2018 03:16 |
| Last Modified: | 30 Nov 2021 00:51 |
| URI: | http://repository.ub.ac.id/id/eprint/9411 |
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