Kriestian, Muhammad (2017) Pengaruh Pemberian Kurkumin Terhadap Kadar Serum Matrix Metalloproteinase 2 (Mmp-2)Dan Tissue Inhibitor Of Metalloproteinase 2 (Timp-2)Pada Proses Resolusi Fibrosis Hati Tikus Model. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang: Fibrosis hati merupakan hasil dari proses fibrogenesis dan fibrolisis. Pada proses fibrogenesis, MMP-2 dan TIMP-2 yang diproduksi sel stellata hati (HSC), mengatur homeostasis matriks ekstraseluler (MES). Kurkumin menghambat ekspresi MMP-2 dan TIMP-2, serta mendorong apoptosis HSC sehingga menghambat fibrogenesis. Peran kurkumin, MMP-2 dan TIMP-2 dalam proses fibrolisis belum banyak diteliti. Tujuan: Mengetahui hubungan antara pemberian kurkumin terhadap penurunan MMP-2 dan TIMP-2 pada tikus model fibrosis hati. Metode: Penelitian eksperimental pada tikus jantan strain wistar. Terdapat 8 kelompok, masing-masing terdiri dari 4 tikus. Kelompok KP dipapar CCl4 1cc/kgbb 2x perminggu intraperitoneal selama 9 minggu untuk membentuk fibrosis F3. Kelompok KN diinjeksi Normal Salin. Setelah diinjeksi CCl4, Kelompok Kontrol (KK2, KK5, KK9) diberikan plasebo dan Kelompok Perlakuan (KP2, KP5 dan KP9) diberikan kurkumin 200mg/kgbb 2, 5 dan 9 minggu. Dilakukan analisis kadar serum MMP-2 dan TIMP-2 dengan menggunakan uji t independen, uji pengaruh pemberian dan lama pemberian kurkumin dengan ANOVA. Hasil: Terdapat peningkatan signifikan kadar serum MMP-2 dan TIMP-2 pada kelompok KP, dan penurunan signifikan kadar serum MMP-2 dan TIMP-2 pada kelompok KK5 dan KK9. Pada kelompok kurkumin, terdapat penurunan signifikan kadar serum MMP-2 dan TIMP-2 pada KP2, KP5, dan KP9, dan pada perbandingan kelompok plasebo dengan kelompok yang diberikan kurkumin, terdapat penurunan signifikan kadar MMP-2 dan TIMP-2 pada KP2. Kesimpulan: Terdapat penurunan signifikan kadar serum MMP-2 dan TIMP-2 pada pemberian kurkumin selama 2 minggu. Lama pemberian kurkumin berkorelasi dengan penurunan kadar serum TIMP-2 tetapi tidak signifikan pengaruhnya pada kadar serum MMP-2 tikus model fibrosis hati.
English Abstract
Background: Liver fibrosis is a result of fibrogenesis and fibrolysis process. In a fibrogenesis process, MMP-2 and TIMP-2 produced by hepatic stellate cell (HSC), regulate extracellular matrix (ECM) homeostastic. Curcumin inhibit MMP-2 and TIMP-2 expression, and enhance HSC apoptosis, thus inhibit fibrogenesis. Role ofcurcumin, MMP-2, and TIMP-2 in a fibrolysis process has not been widely studied. Objective: To determine correlation between giving of curcumin to the decline of MMP-2 and TIMP-2 on rat model of liver fibrosis. Methods: An experimental study in male rats wistar strain. There are 8 groups, 4 rats for each. KP group was exposed to CCl4 1cc/kg intraperitoneally 2 times per week for 9 weeks to form F3 fibrosis. KN group was injected Normal saline. After CCl4 injected, Control group (KK) was given placebo and intervention group (KP) was given curcumin 200mg/kgBW for 2,5, and 9 weeks. Analysis of MMP-2 and TIMP-2 serum level is conducted by independent t-test, influence and duration of curcumin administration test by ANOVA. Result: MMP-2 and TIMP-2 remarkably increased in KP group, and remarkably decreased in KK5 and KK9 group. There are remarkable increase of MMP-2 and TIMP-2 serum level in KP2, KP5, and KP9, and there is remarkable decrease of MMP-2 and TIMP-2 serum level in KP2 in placebo group comparison with the group given curcumin. Conclusion: There are remarkable decrease of MMP-2 and TIMP-2 serum level after giving of curcumin for 2 weeks. The duration of curcumin administration correlated with decrease of TIMP-2 serum level but not correlated with MMP-2 serum level in rat model of liver fibrosis.
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.324 39/KRI/p/2017/041801073 |
| Uncontrolled Keywords: | CURCUMA, IN FIBROSIS, LIVER, METALLOTUNASES |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.3 Diseases of digestive system > 616.36 Diseases of biliary tract and liver > 616.362 Diseases of liver /Liver--Diseases |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Penyakit Dalam, Fakultas Kedokteran |
| Depositing User: | Nur Cholis |
| Date Deposited: | 13 Apr 2018 02:18 |
| Last Modified: | 21 Oct 2021 01:10 |
| URI: | http://repository.ub.ac.id/id/eprint/9407 |
Preview |
Text
DAFTAR PUSTAKA Kries Revisi.pdf Download (144kB) | Preview |
Preview |
Text
Bab 1 Kries.pdf Download (110kB) | Preview |
Preview |
Text
Lampiran Kries Akhir.pdf Download (614kB) | Preview |
Preview |
Text
Bab 2 Kries.pdf Download (569kB) | Preview |
Preview |
Text
BAB 3 Kries.pdf Download (174kB) | Preview |
Preview |
Text
BAB 4 Kries.pdf Download (329kB) | Preview |
Preview |
Text
BAB 5 Kries.pdf Download (312kB) | Preview |
Preview |
Text
BAB 6 Kries.pdf Download (122kB) | Preview |
Preview |
Text
BAGIAN DEPAN.pdf Download (187kB) | Preview |
Preview |
Text
BAB 7 Kries.pdf Download (58kB) | Preview |
Preview |
Text
Cover TA Kries.pdf Download (80kB) | Preview |
Actions (login required)
 |
View Item |