Pratiwi, Esti Rizkiana (2018) Potensi Actinomycetes Sebagai Penghasil Antibiotik Dari Rhizosfer Pohon Pinus Di Hutan Universitas Brawijaya (Ub Forest), Karangploso, Malang. Sarjana thesis, Universitas Brawijaya.
Abstract
Antibiotik yang dihasilkan oleh Actinomycetes mampu mengatasi penyakit yang disebabkan oleh bakteri patogen MRSA (Methicilin-resistant Staphylococcus aureus). Tujuan dari penelitian ini adalah mempelajari potensi isolat Actinomycetes dari tanah rhizosfer Hutan Universitas Brawijaya (UB Forest) sebagai penghasil antibiotik (Anti-MRSA) dan mengetahui spesies Actinomycetes yang memiliki potensi penghasil antibiotik (Anti-MRSA) berdasarkan sekuen 16S rDNA. Sampel tanah diambil dari bagian rhizosfer pohon Pinus dan Actinoymcetes diisolasi menggunakan medium selektif Starch Casein Agar (SCA). Skrining dan uji potensi dalam menghasilkan antibiotik dilakukan dengan uji antagonis terhadap bakteri Escherichia coli, Staphylococcus aureus dan MRSA menggunakan kultur sel. Uji daya hambat Extracellular metabolite (ECM) dilakukan menggunakan cell-free supernatant dan diujikan pada 6 bakteri patogen (E. coli, EPEC, Vibrio cholerae, S. aureus, S. epidermidis dan MRSA). Data dianalisis ragam dengan α ≤ 0,05. Isolat Actinomycetes diidentifikasi secara molekular menggunakan sekuen 16S rDNA. Sebanyak 16 isolat yang diperoleh, ditemukan tiga isolat yang memiliki potensi penghasil antibiotik (Anti-MRSA) dengan zona hambat dari isolat ACT1.6 (6,1 ± 0,8 mm), ACT3.3 (6,03 ± 0,8 mm) dan ACT5.2 (7,5 ± 1,1 mm). Isolat ACT5.2 teridentifikasi sebagai Streptomyces clavuligerus LCB69.
English Abstract
The antibiotics produced by Actinomycetes are able to overcome the disease that caused by MRSA (Methicilin-resistant Staphylococcus aureus) pathogenic bacteria. The aim of this research is to study the potency of Actinomycetes isolates from soil in Brawijaya University’s forest (UB Forest) as an antibiotic (Anti-MRSA) producers and identify of Actinomycetes species that have the potential as an antibiotic (Anti-MRSA) producer based on 16S rDNA sequences. Soil samples were collected from the rhizosphere of pine trees and the isolation of Actinoymcetes bacteria is using selective medium Starch Casein Agar (SCA). Screening and potency assays of Actinomycetes to produce antibiotics were conducted using the antagonist assay against Escherichia coli, Staphylococcus and MRSA by cell culture. Extracellular metabolite (ECM) Inhibitory assay was conducted using cell-free supernatant and was tested on 6 pathogenic bacteria (E.coli, EPEC, Vibrio cholerae, S. aureus, S. epidermidis and MRSA). Data were analyzed using analysis of variance with α ≤ 0,05. Actinomycetes isolates was identified by molecular identification based on 16S rDNA sequence. There were 16 isolates was obtained, and three of isolates have a potency as an antibiotic producer (Anti-MRSA) by clear zone (inhibitory zone) from isolates ACT1.6 (6,1 ± 0,8 mm), ACT3.3 (6,03 ± 0,8 mm) and ACT5.2 (7,5 ± 1,1 mm).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPIA/2018/25/051800538 |
| Uncontrolled Keywords: | Actinomycetes, antibiotik, metabolit sekunder, Actinoymcetes, antibiotic, secondary metabolite |
| Subjects: | 500 Natural sciences and mathematics > 570 Biology |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 09 Feb 2018 02:08 |
| Last Modified: | 22 Oct 2021 06:33 |
| URI: | http://repository.ub.ac.id/id/eprint/8835 |
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