Rizky, Alfiatus (2017) Optimasi Amobilisasi Xilanase dari Trichoderma viride pada Matriks Bentonit Ca-alginat. Sarjana thesis, Universitas Brawijaya.
Abstract
Xilanase merupakan biokatalis reaksi hidrolisis xilan menjadi xilosa (gula pereduksi). Xilanase amobil mempunyai kondisi lingkungan berbeda dengan xilanase bebasnya, sehingga kondisi kerja optimum amobilisasi xilanase dapat berbeda dengan xilanase bebasnya. Penelitian ini bertujuan menentukan kondisi kerja optimum xilanase amobil yang meliputi kondisi optimum amobilisasi xilanase pada matriks bentonit Ca-alginat serta efisiensi penggunaan ulang amobilisasi dari Trichoderma viride yang diamobilkan dalam matriks bentonit Ca-alginat. Kondisi kerja optimum ditentukan dengan cara menghitung kondisi optimum amobilisasi xilanase pada variasi konsentrasi (2;2,5;3;3,5, dan4). Gula pereduksi ditentukan secara spektrofotometri dengan reagen DNS (dinitrosalisilat). Hasil penelitian menunjukkan bahwa kondisi kerja optimum amobilisasi xilanase dalam matriks bentonit Ca-alginat terjadi pada konsentrasi 3mg/mL dengan aktivitas xilanase 6,115
English Abstract
Xylanase is a biocatalyst of hydrolysis reaction from xylan become xylose (reducing sugar). Immobilized xylanase has a particular environtment condition other than its free xylanase. So the best condition of immobilized xylanase could differ from its free xylanase. The aims of this research is to determined the optimal work condition of immobilized xylanase involving and reused efficiency of immobilized xylanase from Trichoderma viride which immobilized to bentonite Ca-alginate matrix. The optimal work condition was determined by counting the activity of immobilized xylanase which measured at various consentration (2;2,5;3;3,5, and 4). The reducing agent was determined using spectrophotometry by DNS reagent (dinitrosalisilat). The result showed that optimal work condition of immobilized xylanase using bentonite Ca-alginate matrix was occurred at consentration 3mg/mL, with resulting a value of xylanase activity 6,115 μg.g-1minutes-1. Immobilized xylanase can be reuse for three times with remaining activity for about 0,406μg.g- 1minutes-1 (55,45%).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/553/051801060 |
| Uncontrolled Keywords: | Bentonit, amobilisasi xilanase, Trichoderma viride, Bentonite, xylanase immobilized, Trichoderma viride |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.5 Fungi > 579.56 Ascomycotina ( ascomycetes) > 579.567 Pyrenomycetes > 579.567 7 Hypocreales |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 31 Jan 2018 03:02 |
| Last Modified: | 17 Mar 2022 01:54 |
| URI: | http://repository.ub.ac.id/id/eprint/8612 |
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