Hasyati, Noor (2017) Pengaruh Ekstrak Heksana Umbi Bawang Dayak (Eleutherine Palmifolia (L.) Merr.) Terhadap Pencegahan Ulcerative Colitis Pada Mencit Yang Diinduksi Dss (Dextran Sulfate Sodium). Sarjana thesis, Universitas Brawijaya.
Abstract
Senyawa bioaktif pada umbi bawang dayak berupa flavonoid dan turunannya memiliki efek preventif terhadap berbagai macam penyakit, salah satunya Ulcerative Colitis. Untuk mengetahui kemampuan umbi bawang dayak dalam mencegah Ulcerative Colitis maka perlu dilakukan ekstraksi selanjutnya diujikan pada hewan coba. Sehingga tujuan dari penelitian ini yaitu mengetahui pengaruh ekstrak heksana umbi bawang dayak dalam upaya pencegahan ulcerative colitis. Uji in vivo menggunakan tiga kelompok mencit yaitu kontrol positif, kontrol negatif, pemberian ekstrak dosis 750mg/kgBB dimana masing-masing perlakuan terdiri dari 6 ekor mencit. Analisa data menggunakan ANOVA dilanjutkan dengan uji Tukey untuk data yang signifikan. Data non parametrik menggunakan kruskal wallis test dilanjutkan dengan Dunn’s test untuk hasil analisa yang signifikan. Hasil penelitian menunjukkan bahwa Ekstrak heksana memiliki nilai aktivitas antioksidan 72,817%, nilai total fenol 327,464mg GAE/100g sampel, nilai kadar flavonoid 2.955,705mg QE/100g sampel. Induksi DSS mampu menimbulkan ulcerative colitis ditunjukkan dengan adanya peningkatan MDA dan penurunan SOD yang signifikan (P <0,05). Namun belum mampu meningkatkan berat kolon, rasio berat per panjang kolon, dan skor mikroskopis. Pengaruh ekstrak mampu menurunkan SOD dan MDA secara signifikan (P <0,05) namun belum mampu memberikan efek yang signifikan pada pengamatan makroskopis dan skor mikroskopis. Kesimpulan dari penelitian ini yaitu induksi dengan DSS 2% mampu menimbulkan ulcerative colitis. Dosis ekstrak 750mg/kgBB belum mampu secara signifikan mencegah ulcerative colitis.
English Abstract
Bioactive compounds in dayak onion bulbs such as flavonoids and its derivatives have preventive effects against various diseases, such as ulcerative colitis. To determine the potency of bawang dayang bulbs to prevent ulcerative colitis it should be extracted followed by testing the extract in the experimental animals. The aim of this study is to the effect of dayak onion bulbs hexane extract in the prevention of ulcerative colitis. In vivo test consist of three groups control positive, control negative and extract dose 750mg/weight each groups consisted 6 mice. Analysis data in vivo used ANOVA followed by Tukey test for significant data. Kruskal wallis was used for non-parametric data followed by Dunn’s test if the result was significant. The result showed that hexane extract of dayak onion had antioxidant activity 72,817%, total pnenolic 327,464mg GAE/100g samples, flavonoid content 2.955,705mg QE/100g samples. DSS induced able to trigger ulcerative colitis which is shown with increasing MDA and decreasing SOD significantly (P <0,05). However, it had not been able to increase weight colon, weight per length colon ratio, and microscopy score between positive and negative control. Effect of the extract significantly able to reduce SOD and MDA (P <0,05) but has not been able to provide a significant effect on acroscopic and microscopic colon. As conclusion, DSS induced could trigger ulcerative colitis. Extract dose 750mg/weight was not able to prevent ulcerative colitis.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FTP/2017/729/051800356 |
| Uncontrolled Keywords: | Bawang Dayak, DSS, Eleutherine palmifolia (L.) Merr, Ulcerative Colitis |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.324 32 Drugs derived from spesific plant (Aloe vera) > 615.324 33 Drugs derived from spesific plant (Garlic) |
| Divisions: | Fakultas Teknologi Pertanian > Teknologi Hasil Pertanian |
| Depositing User: | Yusuf Dwi N. |
| Date Deposited: | 25 Jan 2018 08:28 |
| Last Modified: | 03 Nov 2021 01:49 |
| URI: | http://repository.ub.ac.id/id/eprint/8428 |
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