Astari, Iffa Maulida Wiedy (2017) Pengaruh Ekstrak Etanol Daun Tembakau Terhadap Ketebalan Dinding Pembuluh Darah Arteri Ekor Model Tikus Diabetes Mellitus Tipe II. Sarjana thesis, Universitas Brawijaya.
Abstract
AteroskIerosis sebagai kompIikasi Diabetes MeIitus Tipe 2 dapat terjadi akibat interaksi ROS dengan LDL yang menghasiIkan LDL teroksidasi. SeteIah terbentuknya LDL teroksidasi, terjadi peningkatan transmigrasi seI infIamasi ke dinding pembuIuh darah dan menyebabkan penebaIan. Ditemukan peneIitian, bahwa nikotin dapat berfungsi sebagai antioksidan dengan menghambat ROS. Jika ROS dapat dihambat, maka LDL tidak dapat teroksidasi oIeh ROS. Dengan demikian, penebaIan dinding pembuIuh darah akibat ateroskIerosis pada DM tipe 2 dapat dihambat. Nikotin dapat dengan mudah didapatkan dari tanaman tembakau. Kandungan nikotin daIam daun tembakau adaIah 5,5%. PeneIitian ini menggunakan teknik in vivo true experimentaI and randomized post test onIy controIIed group pada tikus gaIur wistar jantan Rattus norvegicus untuk mengetahui pengaruh ekstrak etanol daun tembakau terhadap ketebaIan pembuIuh darah arteri ekor tikus model diabetes melitus tipe 2. Diabetes MeIitus tipe 2 diinduksi dengan pemberian high fatty diet dan injeksi Streptozotocin dosis rendah 22,5mg/kgBB intraperitoneaI. PeneIitian ini terdiri dari 5 keIompok tikus dengan jumIah 5 tikus per keIompoknya. KeIompok I adaIah kontroI negatif, keIompok II adaIah kontroI positif. keIompok III, IV, V merupakan keIompok perIakuan dengan masing-masing diberi ekstrak etanoI daun tembakau Nicotiana tabacum per oraI dengan dosis 90mg/kg, 180mg/kg, 270mg/kg. Hari ke-46 tikus dibedah dan diambiI potongan arteri ekornya untuk pemeriksaan histopatoIogis. KetebaIan arteri ekor diperiksa dengan perbesaran mikroskop 40x membentuk garis tegak Iurus dengan dinding Iuar pembuIuh darah pada 5 zona, menghitung ketebaIan Iapisan tunika intima hingga tunika adventisia. HasiI pengukuran dan analisis data menunjukkan penebaIan meningkat signifikan pada keIompok kontroI positif, dan menurun signifikan pada keIompok III, IV, V.
English Abstract
AtheroscIerosis as a complication of Type 2 Diabetes MelIitus may occur due to interaction between ROS and LDL that result in oxidized LDL. After the formation of oxidized LDL, transmigration of inflammation cell increases into the blood vessel wall and causing wall thickening. It has been found that nicotine can be an antioxidant by inhibiting ROS. If ROS is inhibited, then LDL can not be oxidized by ROS. Thus, the wall thickening due to atheroscIerosis on Type 2 DM can be inhibited. Nicotine can be easily obtained from tobacco plants. Tobacco’s leaves contain 5,5% nicotine. This study uses “in vivo true experimentaI technique and randomized post test onIy controIIed group” in male rat Rattus norvegicus to know the effect of ethanol extract of tobacco’s leaves to the wall of artery blood vessel in rat Type 2 DM. Type 2 DM is induced by giving high fatty diet and low dose Streptozotocin 22,5mg/kg intraperitoneal injection. The study consisted of 5 groups of rat with a total of 5 rats per group. Group I is a negative control, group II is a positive control. Group III, IV, V are the group of treatment with each group is given ethanol extract of tobacco’s leaves (Nicotiana tabacum) per oral at doses of 90mg/kg, 180mg/kg, 270mg/kg. At the 46th day, the rats were dissected and the tail artery blood vessel was taken for histopathologic examination. The thickness of the tail artery was examined by microscope with 40x magnification, forming a perpendicular line with outer wall of blood vessels in 5 zones, calculating the thickness of the intima tunica layer to the adventitia tunica layer. The result of measurement and data analysis showed significant improvement in the positive control group, and decreased significantly in groups III, IV, and V.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2017/438/051712493 |
| Uncontrolled Keywords: | Aterosklerosis, Diabetes Mellitus Tipe II, Daun tembakau, Ketebalan arteri ekor, Atherosclerosis, Type II Diabetes Mellitus, Tobacco’s Leaves, Tail artery thickness |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.4 Diseases of endocrine, hematopoietic, lymphatic, glandular system; diseases of male breast > 616.46 Diseases of islands of Langerhans > 616.462 Diabetes mellitus > 616.462 061 Diabetes--Chemotherapy / Hypoglycemic agents |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 18 Jan 2018 03:49 |
| Last Modified: | 15 Sep 2020 07:41 |
| URI: | http://repository.ub.ac.id/id/eprint/8113 |
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