Pambudi, Dio Labda (2017) Aktivitas Antibakteri Ekstrak Kasar Cacing Laut Nereis Sp., Cacing Tanah Eisenia Foetida Dan Cacing Tanah Lumbricus Rubellus Dengan Pelarut Etil Asetat Terhadap Bakteri Enterococcus Faecalis. Sarjana thesis, Universitas Brawijaya.
Abstract
Enterococcus faecalis dicurigai menjadi penyebab penyakit gastroenteritis, bakteriemia, endokarditis, meningitis, infeksi uriner, dan bermacam-macam penyakit infeksi yang lain. Penelitian terdahulu telah menunjukkan bahwa pada cacing laut Nereis sp., cacing tanah Eisenia foetida dan cacing tanah Lumbricus rubellus memiliki senyawa antibakteri. Beberapa cara untuk mengetahui ada atau tidaknya aktivitas antibakteri pada ekstrak cacing dengan dilakukan uji daya hambat, MIC dan MBC, Pengaruh paparan ekstrak terhadap bakteri diamati dengan mikroskop cahaya dan scanning electron microscope. Hasil penelitian ini menunjukkan bahwa ekstrak Nereis sp., Eisenia foetida dan Lumbricus rubellus memiliki aktivitas antibakteri terhadap bakteri Enterococcus faecalis. Cacing tanah Lumbricus rubellus memiliki zona bening paling besar yaitu 2,244 mm. Nilai MIC ekstrak Nereis sp. pada konsentrasi 50.000 ppm, Eisenia foetida pada konsentrasi 12.500 ppm dan Lumbricus rubellus pada konsentrasi 12.500 ppm. Hasil uji MBC menunjukkan bahwa ketiga ekstrak cacing bersifat bakteriostatik. Hal ini ditandai dengan kekeruhan akibat tumbuhnya bakteri. Pengamatan bakteri Enterococcus faecalis yang terpapar ketiga ekstrak cacing dengan SEM perbesaran 15.000x menunjukkan sedikit kerusakan sel bakteri berupa kerutan.
English Abstract
Enterococcus faecalis is suspected to be the cause of disease gastroenteritis, bacteremia, endocarditis, meningitis, urinary infections, and a variety of other infectious diseases. Past research has shown that the marine worm Nereis sp., Earthworm Eisenia foetida and earthworm Lumbricusrubellus have antibacterial compounds. Some ways to determine whether or not the antibacterial activity of the extracts tested the worm with inhibition, MIC and MBC, Effect of exposure to the bacteria extract was observed by light microscopy and scanning electron microscope. The results showed that the extract Nereis sp., Eisenia foetida and Lumbricus rubellus possess antibacterial activity against bacteria Enterococcus faecalis. Earthworms Lumbricus rubellus have the most clear zone that is 2,244 mm. MIC values of extracts Nereis sp. at a concentration of 50,000 ppm, Eisenia foetida at a concentration of 12,500 ppm and Lumbricus rubellus on concentration 12,500 ppm. From MBC test showed that these extracts of the worms were bacteriostatic substances. It characterized by turbidity due to bacterial growth. Exposured these extracts on Enterococcus faecalis using SEM with 15.000x magnification showed only a little damages forming wrinkles of the bacteria cell.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FPR/2017/920/051712285 |
| Uncontrolled Keywords: | - |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.36 Drugs of animal origin |
| Divisions: | Fakultas Perikanan dan Ilmu Kelautan > Manajemen Sumberdaya Perairan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 18 Dec 2017 01:13 |
| Last Modified: | 14 Aug 2020 04:27 |
| URI: | http://repository.ub.ac.id/id/eprint/7122 |
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