Noor, Defira Meilyana (2017) Ekspresi Interleukin-5 Dan Interleukin-10 Pada Sel Limfosit Jaringan Paru Hewan Tikus Putih (Rattusnorvegicus) Model Asma Hasil Induksi Ovalbumin Dan Lipopolisakarida (Lps) Setelah Terapi Ekstrak Buah Mengkudu (Morinda Citrifolia). Sarjana thesis, Universitas Brawijaya.
Abstract
Asma adalah penyakit inflamasi kronis pada saluran pernapasan. Angka prevalensi asma pada hewan kesayangan seperti anjing dan kucing sangatlah tinggi. Ekstrak buah Mengkudu (Morinda citrifolia L.) digunakan sebagai terapi pada hewan model tikus asma. Tujuan penelitian ini untuk mempelajari potensi ekstrak buah mengkudu (Morinda citrifolia L.) sebagai terapi penyakit asma yang dilihat melalui ekspresi Interleukin-5 (IL-5) dan Interleukin-10 (IL-10) sel limfosit dari organ paru dengan metode imunohistokimia. Tikus model asma disiapkan dengan pemberian sensitasi alergi dengan Ovalbumin (OVA) secara intraperitoneal dan inhalasi serta pemberian Lipopolisakarida (LPS) dari Phorphyromonas gingivalis secara intrasulkuler. Percobaan dilakukan dengan menggunakan empat kelompok tikus, yaitu kelompok P1 (kontrol negatif), P2 (kontrol positif), P3 (asma+terapi ekstrak buah mengkudu dosis 50 mg/kgBB), dan P4 (asma+terapi ekstrak buah mengkudu dosis 75 mg/kgBB). Ekspresi IL-5 dan IL- 10 didukung dengan metode Imunohistokimia. Hasil perhitungan jumlah sel limfosit Ekspresi Interleukin-5 dan Interleukin -10 dihitung menggunakan software Axiovision kemudian dianalisa dengan ANOVA.. Hasil penelitian ini menunjukkan bahwa ekstrak buah mengkudu menurunkan ekspresi IL-5 secara signifikan (p<0,05) serta menaikkan ekspresi IL-10. Dosis 75 mg/kg BB merupakan dosis optimal ekstrak Buah Mengkudu untuk terapi asma. Kesimpulan dari penelitian ini adalah ekstrak Buah Mengkudu (Morinda citrifolia Linn.) mampu menurunkan ekspresi IL-5 dan menaikkan ekspresi IL-10 sel Limfosit organ Paru sehingga dapat digunakan untuk terapi asma
English Abstract
Asthma is a respiratory disease caused by chronic inflammation. It has been reported that prevalence of asthma in pets (i.e dog and cat) is very high. The Morinda citrifolia L. fruit extract were used as an therapy agent on asthma rats. The purpose of this research was to study the potential of Morinda citrifolia L fruit extract that could affect to expression of Interleukin-5 (IL-5) and Interleukin-10 (IL-10) that count by immuohistochemistry method. Asthma rats were prepare by sensitization of allergent conducted by intraperitonial injection and nebulized of Ovalbumin (OVA) also intrasulcular injection of Lipopolysaccharide from Phorphyromonas gingivalis bacteria. Four groups of rats (Rattus norvegicus) were used in this research were P1 (negative control), P2 (positive control), P3 (asthma + therapy with Morinda citrifolia L extract dose 50 mg/kgWB) and P4 (asthma + therapy with Morinda citrifolia L extract dose 50 mg/kgWB). Expression of IL-5 and IL-10 were Analyze by Imunohistochemistry techniques. The calculation result of lymphocyte cell count th expression of Interleukin-5 (IL-5) and Interleukin-10 (IL-10) was measured using the AxioVision software then analyzed by ANOVA. The results showed that Morinda citrifolia fruit extract therapy decreased expression of IL-5 significantly (p<0.05) and increased espression of IL-10. Morinda citrifolia fruit extract dose of 75 mg/kg BW was the optimal dose for asthma therapy. It is concluded that morinda citrifolia fruit extract could reduce the expression of IL-5 and increase the expression lymphocyte cell Lung tissue so it can be used for the treatment of asthma.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2017/179/051711667 |
| Uncontrolled Keywords: | Buah Mengkudu, Asma, IL-5, dan IL-10, OVA, Sel Limfosit |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.2 Diseases of respiratory system > 616.23 Diseases of trachea and bronchi > 616.238 Asthma |
| Divisions: | Profesi Kedokteran > Spesialis Dokter Hewan, Fakultas Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 16 Nov 2017 02:07 |
| Last Modified: | 08 Oct 2020 04:32 |
| URI: | http://repository.ub.ac.id/id/eprint/5589 |
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