Kharisma, Viol Dhea (2017) Epitope Mapping Daerah Conserved Pada Kapsid L1 Human Papillomavirus Sebagai Pengembangan Vaksin Pencegahan Kanker Serviks. Sarjana thesis, Universitas Brawijaya.
Abstract
Human papillomavirus (HPV) merupakan virus yang memainkan peran penting dalam terjadinya kanker serviks, HPV adalah anggota famili papoviridae, genus papillomavirus. Gen HPV terdiri atas 2 bagian yaitu early dan late gene. Protein early pada HPV meliputi E1, E2, E4, E5, E6, dan E7 Late gene yang terekspresi dari late promoter berperan sebagai pengkode protein kapsid L1 dan L2. Protein L1 memiliki region conserved yang tersusun oleh residu cystein dan lysine, kedua residu tersebut memiliki keterlibatan dalam proses pengikatan antara virion dengan reseptor host. Penelitian sebelumnya menunjukkan bahwa vaksin dapat dikembangkan berdasarkan epitop yang memiliki daerah conserved. Penelitian ini penting dilakukan untuk mengidentifikasi sekuens protein conserved pada L1 kapsid HPV, memprediksi epitope mapping sel B serta antigenisitas pada daerah conserved kapsid L1 HPV, serta kesamaan residu asam amino penyusun epitop dengan reseptor permukaan sel tubuh manusia. Daerah conserved teridentifikasi pada L1 HPV yang berpotensi sebagai epitop sel B berdasarkan analisis epitope mapping yaitu posisi 23- 46 dan 97-119 dengan peptida EGRGQPLGGSGHPNDDEDRDKQ dan RHNGGPGPSGSSQFNKPYWAQGN serta masing-masing memiliki panjang peptida sebesar 22-mer dan 23-mer. Epitop dengan posisi 97-119 memiliki skor antigenisitas tinggi dan kesamaan urutan residu asam amino yang rendah, terhadap reseptor permukaan sel tubuh manusia. Jadi peptida RHNGGPGPSGSSQFNKPYWAQGN dengan panjang 23-mer dapat digunakan sebagai rujukan pengembangan vaksin pencegahan kanker serviks.
English Abstract
Human papillomavirus (HPV) is a virus that plays an important role in the occurrence of cervical cancer, HPV is a member of the family papoviridae, genus papillomavirus. The HPV gene consists of 2 parts, the early and late gene. Early proteins in HPV include E1, E2, E4, E5, E6, and E7 Late gene expressed from the late promoter acting as coders for L1 and L2 protein capsid. The L1 protein has a conserved region composed of cysteine and lysine residues, both of which have involved in the binding process between virions and host receptors. Previous research has shown that vaccines can be developed based on epitopes that have conserved areas. This research is important to identify conserved protein sequences in L1 of HPV capsid, predict epitope mapping of B cells as well as antigenicity in the conserved region of L1 HPV capsid, as well as similarity of amino acid residue of epitope compound with surface receptor of human body cell. The conserved areas were identified in L1 HPV as a potential epitope of B cells based on epitope mapping analysis of positions 23-46 and 97-119 with EGRGQPLGGSGHPNDDEDRDKQ peptides and RHNGGPGPSG SSQFNKPYW AQGN and each had a peptide length of 22-mer and 23- mer. The 97-119 epitope has a high antigenicity score and low similarity of amino acid residue sequence to the cell surface receptor in the human body. So the 23-mer RHNGGPGPSGSSQFNKPYWAQGN peptide can be used as a reference for the development of cervical cancer prevention vaccine.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/513/051710015 |
| Uncontrolled Keywords: | Conserved, epitope mapping, HPV, peptida, L1 |
| Subjects: | 500 Natural sciences and mathematics > 570 Biology |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 06 Nov 2017 07:16 |
| Last Modified: | 17 Nov 2021 02:28 |
| URI: | http://repository.ub.ac.id/id/eprint/4930 |
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