Restiyan, Nurwichdatul Lilla (2017) Pengaruh Pemberian Ekstrak n-heksan Akar Pletekan (Ruellia tuberosa L.) terhadap Penurunan Kadar Glukosa, Aktivitas Enzim Protease dan Profil Protein pada Hepar Tikus Putih (Rattus norvegicus) Diabetes Melitus Tipe 1 Pasca Induksi MLD-STZ. Sarjana thesis, Universitas Brawijaya.
Abstract
Diabetes Melitus (DM) merupakan kelainan kronis yang ditandai dengan gangguan metabolisme karbohidrat yang disebabkan defisiensi insulin baik absolut maupun relatif. Defisiensi insulin absolut ditemukan pada penderita DM tipe 1 yang disebabkan kerusakan sel β pankreas. Kerusakan ini menyebabkan produksi insulin berkurang, akibatnya terjadi peningkatan kadar glukosa dalam darah (hiperglikemia). Penelitian ini bertujuan untuk mengetahui potensi terapi dari ekstrak n-heksan akar pletekan (Ruellia tuberosa) pada hewan coba tikus putih (Rattus norvgicus) yang diinduksi MLDSTZ. Parameter yang diamati pada penelitian ini adalah kadar glukosa darah, aktivitas protease, dan profil protein dari organ hepar tikus. Pengukuran kadar glukosa darah dilakukan menggunakan alat glukometer, aktivitas protease diukur dengan metode spektrofotometri UV, dan analisa profil protein dilakukan dengan meode elektroforesis SDS-PAGE. Hasil penelitian menunjukkan penurunan kadar glukosa darah sebesar 60,29% dan penurunan aktivitas protease sebesar 44,87%. pada tikus kelompok terapi ekstrak n-heksan akar pletekan dosis 250 mg/kgBB terhadap kelompok positif DM tipe 1. Pada hasil analisa profil protein, terapi ekstrak n-heksan akar pletekan memberikan pengaruh hilangnya protein dengan berat molekul 70 kDa yang muncul pada tikus kelompok positif DM tipe 1, dimana protein tersebut diduga merupakan protein HSP.
English Abstract
Diabetes Mellitus (DM) is a chronic disease characterized by carbohydrate metabolism disorders caused by both absolute and relative insulin deficiency. Absolute insulin deficiency is found in human with type 1 diabetes which caused by destruction of pancreatic β cells. This damage causes reduced production of insulin, resulting in increase glucose levels in blood (hyperglycemia). The aim of this research was to investigate the therapeutic potential of n-hexane pletekan's root extract (Ruellia tuberosa) in rats (Rattus norvgicus) induced by MLD-STZ. The parameters observed in this study were glucose levels in blood, protease activities, and protein profile of diabetic rat’s liver. Blood glucose levels were measured using glucometer, protease activities was measured by spectrophotometric UV method, and protein profile analysis was performed by electrophoresis SDS-PAGE method. The results of this study showed that the decrease of blood glucose level was 60.29% and showed the decreased of protease activites up to 44.87% in group of n-hexane root extract therapy with dose 250 mg / kgBW that compared with positive group of type 1 DM. Results of protein profile analysis with SDSPAGE electrophoresis method, showed loss of protein with molecular weight of 70 kDa, where the protein was suspected to be HSP protein.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/498/051709825 |
| Uncontrolled Keywords: | Diabetes Melitus, insulin, MLD-STZ, aktivitas protease, profil protein, protein HSP |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 95 Drugs derived from spesific plant (Scrophulariales) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 06 Nov 2017 03:22 |
| Last Modified: | 17 Nov 2021 04:03 |
| URI: | http://repository.ub.ac.id/id/eprint/4890 |
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