Prabowo, Gempar Aditya (2017) Pengaruh Suhu dan Lama Penyimpanan terhadap Kestabilan Aktivitas Xilanase Trichoderma viride Hasil Filtrasi Gel yang Diamobil pada Matriks Zeolit dan Efisiensi Pemakaian Ulang. Sarjana thesis, Universitas Brawijaya.
Abstract
Xilanase termasuk enzim hidrolase yang memiliki kemampuan untuk menghidrolisis xilan menjadi xilosa. Xilanase dapat diisolasi dari biakan jamur Trichoderma viride dengan bantuan induser (xilan) yang kemudian melalui serangkaian proses pemurnian antara lain, pengendapan menggunakan amonium sulfat dengan tingkat kejenuhan 40-80%, dialisis menggunakan kantong selofan dan kromatografi filtrasi gel. Amobilisasi xilanase dapat meningkatkan stabilitas dari pengaruh lingkungan seperti suhu dan pH. Xilanase diamobilisasi menggunakan matriks zeolit dengan metode adsorpsi fisik. Penelitian ini bertujuan untuk mempelajari pengaruh suhu pada variasi 0oC (freezer), 4oC (refrigerator), 27oC, 50oC dan lama penyimpanan selama (0, 1, 2, 3, 4, 5, 6, 7) hari terhadap aktivitas xilanase amobil dan efisiensi pemakaian enzim xilanase amobil. Aktivitas xilanase ditentukan dengan cara mengukur kadar gula pereduksi secara spektrofotometri menggunakan reagen DNS. Hasil penelitian menunjukkan bahwa semakin lama waktu penyimpanan aktivitas xilanase amobil semakin menurun. Pada pengaruh suhu, xilanase amobil stabil pada suhu 4oC (refrigerator) dengan aktivitas enzim pada penyimpanan hingga hari ke 4 sebesar 58,75%.
English Abstract
Xylanases belongs to hydrolase enzymes that have the ability to hydrolyze xylan into xylose. The xylanase can be isolated from the Trichoderma viride mushroom’s culture with the help of the inducer (xylan) which furthermore through a series of purification processes, i.e. the precipitation using ammonium sulfate with a saturation level of 40-80%, dialysis using cellophane pouch and gel filtration chromatography. The immobilization of xylanase can improve the stability of environmental influences such as temperature and pH. Xylanase is immobilized using zeolite matrix by physical adsorption method. The purpose of this study was to study the effect of temperature on variation of 0oC (freezer), 4oC (refrigerator), 27oC, 50oC and storage duration during (0, 1, 2, 3, 4, 5, 6, 7) days to xylanase activity of filtration gel and the efficiency of immobilized xylanase enzymes. The activity of xylanase was determined by measuring the spectrophotometric reducing sugar content using a DNS reagent. The results showed that the longer storage time of immobilized xylanase activity decreased. At the influence of temperature, immobilized xylanase was stable at 4oC (refrigerator) with enzyme activity in storage up to day 4 was 58,75%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/450/051709790 |
| Uncontrolled Keywords: | Xilanase, Amobilisasi, Kestabilan, Suhu, Lama Penyimpanan, Efisiensi. |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.5 Fungi > 579.56 Ascomycotina ( ascomycetes) > 579.567 Pyrenomycetes > 579.567 7 Hypocreales |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 31 Oct 2017 07:26 |
| Last Modified: | 18 Nov 2021 04:19 |
| URI: | http://repository.ub.ac.id/id/eprint/4633 |
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