Zahara, Nadinne Ilma (2017) Pengaruh Pemberian Ekstrak Kulit Semangka (Citrullus Lanatus) Terhadap Kadar Malondialdehid Hepar Pada Tikus Wistar Jantan Dengan Diet Tinggi Lemak. Sarjana thesis, Universitas Brawijaya.
Abstract
Diet tinggi lemak dapat mengganggu keseimbangan mekanisme lemak dalam tubuh. Diet tinggi lemak dapat menyebabkan akumulasi trigliserida di hepar, bila hal tersebut dibiarkan maka dapat menyebabkan fatty liver. Asam lemak bebas yang meningkat akan menginduksi inflamasi sehingga radikal bebas meningkat dan menyebabkan peroksidasi lipid. Peroksidasi lipid menghasilkan produk antara lain malondialdehid (MDA). Peroksidasi lipid dapat dihambat dengan menggunakan antioksidan dalam menangkal radikal bebas sehingga patogenesis NAFLD terhambat. Antioksidan banyak ditemukan pada buah dan sayur, salah satunya semangka dimana bagian kulitnya mempunyai antioksidan antara lain flavonoid. Penelitian ini bertujuan untuk membuktikan pengaruh pemberian ekstrak kulit semangka untuk menurunkan kadar MDA hepar pada tikus wistar jantan dengan diet tinggi lemak. Penelitian ini merupakan studi eksperimental menggunakan post test control group design. Dua puluh lima tikus dibagi secara acak menjadi 5 kelompok, terdiri atas kelompok kontrol negatif dengan diet standar (KN), kelompok kontrol positif dengan diet tinggi lemak (KP), kelompok perlakuan 1 dengan diet tinggi lemak dan ekstrak kulit semangka 250 mg/kgBB/hari (P1), kelompok perlakuan 2 dengan diet tinggi lemak dan ekstrak kulit semangka 500 mg/kgBB/hari (P2), dan kelompok perlakuan 3 dengan diet tinggi lemak dan simvastatin 0,9 mg/kgBB/hari (P3). Setelah perlakuan 9 minggu, hewan coba dibedah dan diambil organ heparnya. Pengukuran kadar MDA hepar dilakukan dengan menggunakan spektrofotometri. Berdasarkan uji one way ANOVA, didapatkan bahwa p<0,05. Uji post hoc juga menunjukkan bahwa ekstrak kulit semangka dosis 500 mg/kgBB/hari mampu menurunkan kadar MDA hepar sebaik simvastatin dosis 0,9 mg/kgBB/hari.
English Abstract
A high-fat diet can disrupt the balance of fatty mechanisms in the body. High-fat diet causes triglyceride accumulation in the liver, which if left untreated may cause fatty liver. The increase of free fatty acids will induce inflammation, resulting in free radicals increase and causing lipid peroxidation. Lipid peroxidation produces products such as malondialdehyde (MDA). Lipid peroxidation can be inhibited by using antioxidants in counteracting free radicals, so the pathogenesis of NAFLD is inhibited. Antioxidants are usually found in many fruits and vegetables, one of them is watermelon. Watermelon rind has antioxidants such as flavonoids. This study was aimed to prove the effect of watermelon rind extract to reduce hepatic MDA levels in male wistar rats with high-fat diet. This study was an experimental study using post test control group design. Twenty-five rats were randomly divided into 5 groups, consisting of negative control group with standard diet (KN), positive control group with high-fat diet (KP), treatment group 1 with high-fat diet and watermelon rind extract 250 mg/kgBB/day (P1), treatment group 2 with high-fat diet and watermelon rind extract 500 mg/kgBB/day (P2), and treatment group 3 with high fat diet and simvastatin 0.9 mg/kgBW/day (P3). After 9 weeks, the rats were dissected and their liver were taken. Measurements of hepatic MDA levels were performed using spectrophotometry. Based on One Way ANOVA test, it was found that p<0,05. Post hoc test also showed that watermelon rind extract with dose of 500 mg/kgBW/day was able to decrease hepatic MDA level as well as simvastatin with dose of dose 0.9 mg/kgBW/day.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2017/365/051710115 |
| Uncontrolled Keywords: | ekstrak kulit semangka, kadar MDA hepar |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 63 Drugs derived from specific plants (Cucurbitales, Watermelons) |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 25 Oct 2017 01:52 |
| Last Modified: | 24 Sep 2020 04:15 |
| URI: | http://repository.ub.ac.id/id/eprint/4338 |
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