Hastyani, Merry (2017) Karakterisasi Xilanase Hasil Isolasi dari Trichoderma viride yang Diamobilkan pada Matriks Bentonit Teraktivasi HCl. Sarjana thesis, Universitas Brawijaya.
Abstract
Xilanase merupakan biokatalis reaksi hidrolisis xilan menjadi xilosa (gula pereduksi). Xilanase amobil mempunyai kondisi lingkungan berbeda dengan xilanase bebasnya, sehingga kondisi kerja optimum xilanase amobil dapat berbeda dengan xilanase bebasnya. Penelitian ini bertujuan menentukan kondisi kerja optimum xilanase amobil yang meliputi pH, temperatur dan waktu reaksi serta efisiensi penggunaan ulang xilanase amobil dari Trichoderma viride yang diamobilkan dalam matriks bentonit. Kondisi kerja optimum ditentukan dengan cara menghitung aktivitas xilanase amobil yang diukur pada variasi pH (3,4,5,6, dan7), temperatur (40, 50, 60, 70, dan 80) 0C dan waktu reaksi (35,40,45,50,55,60,dan 65) menit. Gula pereduksi ditentukan secara spektrofotometri dengan reagen DNS (dinitrosalisilat). Hasil penelitian menunjukkan bahwa kondisi kerja optimum xilanase yang diamobil dalam matriks bentonit terjadi pada pH 5, temperatur 600C, dan waktu reaksi 55 menit menghasilkan aktivitas xilanase 0,680 μg mg-1 menit -1. Xilanase amobil dapat digunakan kembali sebanyak lima kali pengulangan dengan aktivitas sisa sebesar 0,406 μg.g-1 minutes-1 (55,45%).
English Abstract
Xylanase is a biocatalyst of hydrolysis reaction from xylan become xylose (reducing sugar). Immobilized xylanase has a particular environtment condition other than its free xylanase. So the best condition of immobilized xylanase could differ from its free xylanase. The aims of this research is to determined the optimal work condition of immobilized xylanase involving pH, temperature, reaction time, and reused efficiency of immobilized xylanase from Trichoderma viride which immobilized to bentonite matrix. The optimal work condition was determined by counting the activity of immobilized xylanase which measured at various pH (3,4,5,6, and 7), temperature (40,50,60,70 and 80) and reaction time (35, 40, 45, 50, 55, 60, and 65) minutes. The reducing agent was determined using spectrophotometry by DNS reagent (dinitrosalisilat). The result showed that optimal work condition of immobilized xylanase using bentonite matrix was occurred at pH 5, temperature 55C, and reaction time 55 minutes resulting a value of xylanase activity 0,680 μg.g-1 minutes-1. Immobilized xylanase can be reuse for five times with remaining activity for about 0,406 μg.g-1 minutes-1 (55,45%).
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/334/051709674 |
| Uncontrolled Keywords: | Bentonit, amobilisasi xilanase, Trichoderma viride |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.5 Fungi > 579.56 Ascomycotina ( ascomycetes) > 579.567 Pyrenomycetes > 579.567 7 Hypocreales |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia |
| Depositing User: | Nur Cholis |
| Date Deposited: | 25 Oct 2017 01:25 |
| Last Modified: | 23 Nov 2021 07:07 |
| URI: | http://repository.ub.ac.id/id/eprint/4331 |
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