Ashar, M. Sultonun Arifin Ali (2017) Uji Aktivitas Ekstrak Lemon (Citrus Limon) Terhadap Perubahan Sel T Cd4+Cd62l+ Dan Sel T Cd8+Cd62l+ Pada Mencit (Mus Musculus) Balb/C Betina Model Kanker Payudara. Sarjana thesis, Universitas Brawijaya.
Abstract
Kanker payudara merupakan penyebab utama kematian pada perempuan didunia dan sangat umum dijumpai di negara berkembang. Sistem imun berperan penting dalam mengendalikan perkembangan sel-sel kanker payudara. Salah satu sel limfosit yang berperan dalam mengendalikan pertumbuhan kanker payudara ialah sel T CD4 dan sel T CD8. Lemon (Citrus limon) diketahui memiliki senyawa yang bersifat antikanker dan antiproliferatif. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak lemon (Citrus limon) terhadap sel CD4+CD62L+ dan CD8+CD62L+ pada mencit (Mus muscullus) betina model kanker payudara dengan induksi DMBA (7,12- dimethylbenz(a)anthracen). Terdapat empat perlakuan dengan empat ulangan dalam penelitian ini yaitu normal sehat, DMBA (selama enam minggu), DMBA+ekstrak lemon (selama empat hari) dosis 50 mg/kg BB dan 200 mg/kg BB. Data penelitian dianalisis menggunakan flow cytometry dengan mengambil sel limfosit pada organ spleen. Kemudian dilakukan analisis parametric one way ANOVA dengan selang kepercayaan 95% menggunakan software SPSS versi 16 for Windows lalu dilakukan uji Tukey sebagai uji lanjutan. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak lemon yang diberikan secara oral selama 14 hari pada mencit betina model kaker payudara mampu meningkatkan jumlah relatif sel T CD4+CD62L+ (sel T CD4 naïve) dan sel T CD8+CD62L+ (sel T CD8 naïve). Pemberian ekstrak lemon dosis 50 mg/kg BB mampu meningkatkan jumlah relatif sel T CD4 sebesar 38,51% dan sel T CD8 naïve sebesar 32,65% hingga mendekati normal.
English Abstract
Breast cancer is leading cause of women death in worldwide and it is very common in developing countries. The immune system plays an important role to control growth of breast cancer cells. One of the lymphocyte cells that play a role in control the growth of breast cancer is CD4 T cells and CD8 T cells. Lemon (Citrus limon) is known to have anticancer and antiproliferative compounds. This study aims to determine the effect of Lemon Extract (Citrus limon) on CD4+CD62L+ T cells and CD8+CD62L+ T cells in female mice breast cancer model using DMBA induction (7.12-dimethylbenz (a) anthracen). There are 4 treatments with 4 replications in this study that is normal healthy, DMBA (for 6 weeks), DMBA + lemon extract (for 4 days) dose 50 mg / kg BW and 200 mg / kg BW. The data were analyzed using flow cytometry by taking lymphocyte cells in spleen organ. Then analysis using parametric analysis one way ANOVA with significance value then Tukey test as a follow-up test. Based on this study can be concluded that the extract of lemon given orally in female mice model of breast can increase the relative amount of CD4+CD62L+ T cells (CD4 naïve T cells) and CD8+CD62L+ T cells (CD8 naïve T cells). The treatment of lemon extract dose 50 mg / kg BW was able to increase the relative amount of CD4 of 38.51 % and CD8 naïve T cells of 32.65 % to near normal treatment.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FMIPA/2017/293/051706313 |
| Uncontrolled Keywords: | flow cytometry, hewan model kanker payudara, lemon, sel T |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.99 Tumors and miscellaneous communicable diseases > 616.994 Cancers > 616.994 49 Breast cancer |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 23 Oct 2017 03:50 |
| Last Modified: | 24 Nov 2021 01:57 |
| URI: | http://repository.ub.ac.id/id/eprint/4171 |
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